SSAV_SALTY
ID SSAV_SALTY Reviewed; 681 AA.
AC P74856;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Secretion system apparatus protein SsaV;
GN Name=ssaV; OrderedLocusNames=STM1414;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=LT2;
RX PubMed=9140973; DOI=10.1046/j.1365-2958.1997.3271699.x;
RA Hensel M., Shea J.E., Raupach B., Monack D., Falkow S., Gleeson C.,
RA Kubo T., Holden D.W.;
RT "Functional analysis of ssaJ and the ssaK/U operon, 13 genes encoding
RT components of the type III secretion apparatus of Salmonella pathogenicity
RT island 2.";
RL Mol. Microbiol. 24:155-167(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [3]
RP FUNCTION IN SPVB EXPORT, AND DISRUPTION PHENOTYPE.
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=18248436; DOI=10.1111/j.1574-695x.2007.00364.x;
RA Browne S.H., Hasegawa P., Okamoto S., Fierer J., Guiney D.G.;
RT "Identification of Salmonella SPI-2 secretion system components required
RT for SpvB-mediated cytotoxicity in macrophages and virulence in mice.";
RL FEMS Immunol. Med. Microbiol. 52:194-201(2008).
CC -!- FUNCTION: Component of Salmonella pathogenicity island 2 (SPI-2) type
CC III secretion system, required for secretion of some type III-secreted
CC effectors including the SpvB toxin. {ECO:0000269|PubMed:18248436}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- DISRUPTION PHENOTYPE: Disruption prevents SpvB-induced F-actin
CC depolymerization in human macrophages without affecting intra-bacterial
CC SpvB protein levels. {ECO:0000269|PubMed:18248436}.
CC -!- SIMILARITY: Belongs to the FHIPEP (flagella/HR/invasion proteins export
CC pore) family. {ECO:0000305}.
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DR EMBL; Y09357; CAA70536.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL20338.1; -; Genomic_DNA.
DR RefSeq; NP_460379.1; NC_003197.2.
DR RefSeq; WP_001258231.1; NC_003197.2.
DR PDB; 7AWA; EM; 3.50 A; A=1-681.
DR PDB; 7FEB; EM; 2.11 A; A/B/C/D/E/F/G/H/I=346-681.
DR PDB; 7FEC; EM; 3.64 A; A/B/C/D/E/F/G/H/I=346-681.
DR PDB; 7FED; EM; 3.55 A; A/B/C/D/E/F/G/H/I/J/K/L/M/N/O/P/Q/R=346-681.
DR PDBsum; 7AWA; -.
DR PDBsum; 7FEB; -.
DR PDBsum; 7FEC; -.
DR PDBsum; 7FED; -.
DR AlphaFoldDB; P74856; -.
DR SMR; P74856; -.
DR STRING; 99287.STM1414; -.
DR PaxDb; P74856; -.
DR EnsemblBacteria; AAL20338; AAL20338; STM1414.
DR GeneID; 1252932; -.
DR KEGG; stm:STM1414; -.
DR PATRIC; fig|99287.12.peg.1498; -.
DR HOGENOM; CLU_015346_3_0_6; -.
DR OMA; ECGHLPP; -.
DR PhylomeDB; P74856; -.
DR BioCyc; SENT99287:STM1414-MON; -.
DR PHI-base; PHI:8727; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR Gene3D; 1.10.8.540; -; 1.
DR Gene3D; 3.40.30.60; -; 1.
DR Gene3D; 3.40.50.12790; -; 1.
DR InterPro; IPR042194; FHIPEP_1.
DR InterPro; IPR042193; FHIPEP_3.
DR InterPro; IPR042196; FHIPEP_4.
DR InterPro; IPR025505; FHIPEP_CS.
DR InterPro; IPR001712; T3SS_FHIPEP.
DR InterPro; IPR006302; T3SS_HrcV.
DR PANTHER; PTHR30161; PTHR30161; 1.
DR Pfam; PF00771; FHIPEP; 1.
DR PIRSF; PIRSF005419; FlhA; 1.
DR PRINTS; PR00949; TYPE3IMAPROT.
DR TIGRFAMs; TIGR01399; hrcV; 1.
DR PROSITE; PS00994; FHIPEP; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cell inner membrane; Cell membrane; Membrane;
KW Protein transport; Reference proteome; Transmembrane; Transmembrane helix;
KW Transport.
FT CHAIN 1..681
FT /note="Secretion system apparatus protein SsaV"
FT /id="PRO_0000190034"
FT TRANSMEM 25..45
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 48..68
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 73..93
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 118..138
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 206..226
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 244..264
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 295..315
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 409..429
FT /note="Helical"
FT /evidence="ECO:0000255"
FT STRAND 352..357
FT /evidence="ECO:0007829|PDB:7AWA"
FT HELIX 359..361
FT /evidence="ECO:0007829|PDB:7AWA"
FT HELIX 366..381
FT /evidence="ECO:0007829|PDB:7AWA"
FT STRAND 389..392
FT /evidence="ECO:0007829|PDB:7AWA"
FT STRAND 399..413
FT /evidence="ECO:0007829|PDB:7AWA"
FT STRAND 420..424
FT /evidence="ECO:0007829|PDB:7AWA"
FT STRAND 431..435
FT /evidence="ECO:0007829|PDB:7AWA"
FT HELIX 437..439
FT /evidence="ECO:0007829|PDB:7AWA"
FT STRAND 442..445
FT /evidence="ECO:0007829|PDB:7AWA"
FT HELIX 448..456
FT /evidence="ECO:0007829|PDB:7AWA"
FT HELIX 466..477
FT /evidence="ECO:0007829|PDB:7AWA"
FT HELIX 478..483
FT /evidence="ECO:0007829|PDB:7AWA"
FT HELIX 486..496
FT /evidence="ECO:0007829|PDB:7AWA"
FT TURN 497..499
FT /evidence="ECO:0007829|PDB:7AWA"
FT HELIX 501..507
FT /evidence="ECO:0007829|PDB:7AWA"
FT TURN 508..510
FT /evidence="ECO:0007829|PDB:7AWA"
FT HELIX 513..525
FT /evidence="ECO:0007829|PDB:7AWA"
FT HELIX 533..543
FT /evidence="ECO:0007829|PDB:7AWA"
FT HELIX 544..546
FT /evidence="ECO:0007829|PDB:7AWA"
FT HELIX 550..559
FT /evidence="ECO:0007829|PDB:7AWA"
FT HELIX 562..569
FT /evidence="ECO:0007829|PDB:7AWA"
FT HELIX 571..573
FT /evidence="ECO:0007829|PDB:7AWA"
FT STRAND 576..578
FT /evidence="ECO:0007829|PDB:7AWA"
FT HELIX 583..593
FT /evidence="ECO:0007829|PDB:7AWA"
FT STRAND 597..599
FT /evidence="ECO:0007829|PDB:7AWA"
FT HELIX 606..620
FT /evidence="ECO:0007829|PDB:7AWA"
FT TURN 621..623
FT /evidence="ECO:0007829|PDB:7AWA"
FT TURN 632..634
FT /evidence="ECO:0007829|PDB:7AWA"
FT HELIX 635..642
FT /evidence="ECO:0007829|PDB:7AWA"
FT STRAND 663..665
FT /evidence="ECO:0007829|PDB:7AWA"
SQ SEQUENCE 681 AA; 75322 MW; C9226C9F9A16114A CRC64;
MRSWLGEGVR AQQWLSVCAG RQDMVLATVL LIAIVMMLLP LPTWMVDILI TINLMFSVIL
LLIAIYLSDP LDLSVFPSLL LITTLYRLSL TISTSRLVLL QHNAGNIVDA FGKFVVGGNL
TVGLVVFTII TIVQFIVITK GIERVAEVSA RFSLDGMPGK QMSIDGDLRA GVIDADHART
LRQHVQQESR FLGAMDGAMK FVKGDTIAGI IVVLVNIIGG IIIAIVQYDM SMSEAVHTYS
VLSIGDGLCG QIPSLLISLS AGIIVTRVPG EKRQNLATEL SSQIARQPQS LILTAVVLML
LALIPGFPFI TLAFFSALLA LPIILIRRKK SVVSANGVEA PEKDSMVPGA CPLILRLSPT
LHSADLIRDI DAMRWFLFED TGVPLPEVNI EVLPEPTEKL TVLLYQEPVF SLSIPAQADY
LLIGADASVV GDSQTLPNGM GQICWLTKDM AHKAQGFGLD VFAGSQRISA LLKCVLLRHM
GEFIGVQETR YLMNAMEKNY SELVKELQRQ LPINKIAETL QRLVSERVSI RDLRLIFGTL
IDWAPREKDV LMLTEYVRIA LRRHILRRLN PEGKPLPILR IGEGIENLVR ESIRQTAMGT
YTALSSRHKT QILQLIEQAL KQSAKLFIVT SVDTRRFLRK ITEATLFDVP ILSWQELGEE
SLIQVVESID LSEEELADNE E
