SSBF_ECOLI
ID SSBF_ECOLI Reviewed; 179 AA.
AC P18310;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 3.
DT 25-MAY-2022, entry version 119.
DE RecName: Full=Plasmid-derived single-stranded DNA-binding protein;
DE Short=SSB {ECO:0000255|HAMAP-Rule:MF_00984};
DE AltName: Full=Helix-destabilizing protein;
GN Name=ssbF; Synonyms=ssb, ssf; OrderedLocusNames=ECOK12F060;
OS Escherichia coli (strain K12).
OG Plasmid F.
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=6351061; DOI=10.1073/pnas.80.18.5480;
RA Chase J.W., Merrill B.M., Williams K.R.;
RT "F sex factor encodes a single-stranded DNA binding protein (SSB) with
RT extensive sequence homology to Escherichia coli SSB.";
RL Proc. Natl. Acad. Sci. U.S.A. 80:5480-5484(1983).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / CR63;
RA Shimizu H., Saitoh Y., Suda Y., Uehara K., Sampei G., Mizobuchi K.;
RT "Complete nucleotide sequence of the F plasmid: its implications for
RT organization and diversification of plasmid genomes.";
RL Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP FUNCTION.
RX PubMed=2013585; DOI=10.1128/jb.173.8.2720-2723.1991;
RA Porter R.D., Black S.;
RT "The single-stranded-DNA-binding protein encoded by the Escherichia coli F
RT factor can complement a deletion of the chromosomal ssb gene.";
RL J. Bacteriol. 173:2720-2723(1991).
CC -!- FUNCTION: May contribute to the conjugative processing of DNA. It has a
CC functional relationship with Psi (plasmid-mediated sos inhibition)
CC proteins. {ECO:0000269|PubMed:2013585}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_00984}.
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DR EMBL; K00066; AAA83020.1; -; Genomic_DNA.
DR EMBL; AP001918; BAA97930.1; -; Genomic_DNA.
DR PIR; A20023; DDECF.
DR RefSeq; NP_061439.1; NC_002483.1.
DR RefSeq; WP_000290801.1; NZ_CP014273.1.
DR AlphaFoldDB; P18310; -.
DR SMR; P18310; -.
DR BioGRID; 4054815; 1.
DR PRIDE; P18310; -.
DR PhylomeDB; P18310; -.
DR PRO; PR:P18310; -.
DR GO; GO:0003697; F:single-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR CDD; cd04496; SSB_OBF; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR HAMAP; MF_00984; SSB; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR000424; Primosome_PriB/ssb.
DR InterPro; IPR011344; ssDNA-bd.
DR PANTHER; PTHR10302; PTHR10302; 1.
DR Pfam; PF00436; SSB; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR TIGRFAMs; TIGR00621; ssb; 1.
DR PROSITE; PS50935; SSB; 1.
PE 3: Inferred from homology;
KW DNA replication; DNA-binding; Plasmid.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..179
FT /note="Plasmid-derived single-stranded DNA-binding protein"
FT /id="PRO_0000096042"
FT DOMAIN 6..110
FT /note="SSB"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00984"
FT DNA_BIND 55..61
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00984"
FT REGION 117..179
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 117..136
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 179 AA; 19636 MW; 670D50BA0AD107EE CRC64;
MAVRGINKVI LVGRLGKDPE VRYIPNGGAV ANLQVATSES WRDKQTGEMR EQTEWHRVVL
FGKLAEVAGE CLRKGAQVYI EGQLRTRSWE DNGITRYVTE ILVKTTGTMQ MLVRAAGAQT
QPEEGQQFSG QPQPEPQAEA GTKKGGAKTK GRGRKAAQPE PQPQPPEGDD YGFSDDIPF
