SSBP2_MOUSE
ID SSBP2_MOUSE Reviewed; 361 AA.
AC Q9CYZ8; B1B188; Q99LX9;
DT 10-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Single-stranded DNA-binding protein 2;
DE AltName: Full=Sequence-specific single-stranded-DNA-binding protein 2;
GN Name=Ssbp2; Synonyms=Ssdp2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=NMRI; TISSUE=Brain cortex;
RA Speer N., Tarabykin V., Britanova O., Lukyanov S., Gruss P.;
RT "A novel member of the SSDP gene family, SSDP2, is expressed in deep layers
RT during murine cortical development.";
RL Submitted (JUN-2001) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Embryo;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-321 AND THR-333, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [6]
RP ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-6, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryonic fibroblast;
RX PubMed=23806337; DOI=10.1016/j.molcel.2013.06.001;
RA Park J., Chen Y., Tishkoff D.X., Peng C., Tan M., Dai L., Xie Z., Zhang Y.,
RA Zwaans B.M., Skinner M.E., Lombard D.B., Zhao Y.;
RT "SIRT5-mediated lysine desuccinylation impacts diverse metabolic
RT pathways.";
RL Mol. Cell 50:919-930(2013).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Embryo;
RX PubMed=24129315; DOI=10.1074/mcp.o113.027870;
RA Guo A., Gu H., Zhou J., Mulhern D., Wang Y., Lee K.A., Yang V., Aguiar M.,
RA Kornhauser J., Jia X., Ren J., Beausoleil S.A., Silva J.C., Vemulapalli V.,
RA Bedford M.T., Comb M.J.;
RT "Immunoaffinity enrichment and mass spectrometry analysis of protein
RT methylation.";
RL Mol. Cell. Proteomics 13:372-387(2014).
CC -!- INTERACTION:
CC Q9CYZ8; Q9WUH2: Lhx9; NbExp=3; IntAct=EBI-309962, EBI-309943;
CC Q9CYZ8; Q60769: Tnfaip3; NbExp=2; IntAct=EBI-309962, EBI-646595;
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9CYZ8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9CYZ8-2; Sequence=VSP_006259;
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DR EMBL; AY037837; AAK71881.1; -; mRNA.
DR EMBL; AK013177; BAB28693.1; -; mRNA.
DR EMBL; AC154320; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CT009700; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC002176; AAH02176.1; -; mRNA.
DR CCDS; CCDS26676.1; -. [Q9CYZ8-2]
DR CCDS; CCDS26677.1; -. [Q9CYZ8-1]
DR RefSeq; NP_077148.1; NM_024186.5. [Q9CYZ8-2]
DR RefSeq; NP_077234.2; NM_024272.4. [Q9CYZ8-1]
DR AlphaFoldDB; Q9CYZ8; -.
DR SMR; Q9CYZ8; -.
DR BioGRID; 211844; 18.
DR IntAct; Q9CYZ8; 20.
DR MINT; Q9CYZ8; -.
DR STRING; 10090.ENSMUSP00000004094; -.
DR iPTMnet; Q9CYZ8; -.
DR PhosphoSitePlus; Q9CYZ8; -.
DR EPD; Q9CYZ8; -.
DR MaxQB; Q9CYZ8; -.
DR PaxDb; Q9CYZ8; -.
DR PRIDE; Q9CYZ8; -.
DR ProteomicsDB; 258734; -. [Q9CYZ8-1]
DR ProteomicsDB; 258735; -. [Q9CYZ8-2]
DR Antibodypedia; 12817; 199 antibodies from 32 providers.
DR DNASU; 66970; -.
DR Ensembl; ENSMUST00000004094; ENSMUSP00000004094; ENSMUSG00000003992. [Q9CYZ8-1]
DR Ensembl; ENSMUST00000042122; ENSMUSP00000037115; ENSMUSG00000003992. [Q9CYZ8-2]
DR GeneID; 66970; -.
DR KEGG; mmu:66970; -.
DR UCSC; uc007rjv.2; mouse. [Q9CYZ8-1]
DR UCSC; uc007rjw.2; mouse. [Q9CYZ8-2]
DR CTD; 23635; -.
DR MGI; MGI:1914220; Ssbp2.
DR VEuPathDB; HostDB:ENSMUSG00000003992; -.
DR eggNOG; KOG4594; Eukaryota.
DR GeneTree; ENSGT00950000183049; -.
DR HOGENOM; CLU_053914_1_0_1; -.
DR InParanoid; Q9CYZ8; -.
DR OMA; IMPSPAX; -.
DR OrthoDB; 907486at2759; -.
DR PhylomeDB; Q9CYZ8; -.
DR TreeFam; TF318961; -.
DR BioGRID-ORCS; 66970; 0 hits in 73 CRISPR screens.
DR ChiTaRS; Ssbp2; mouse.
DR PRO; PR:Q9CYZ8; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; Q9CYZ8; protein.
DR Bgee; ENSMUSG00000003992; Expressed in metanephric mesenchyme and 268 other tissues.
DR ExpressionAtlas; Q9CYZ8; baseline and differential.
DR Genevisible; Q9CYZ8; MM.
DR GO; GO:0005737; C:cytoplasm; ISO:MGI.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003697; F:single-stranded DNA binding; IEA:InterPro.
DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR InterPro; IPR006594; LisH.
DR InterPro; IPR008116; SSDP_DNA-bd.
DR PRINTS; PR01743; SSDNABINDING.
DR SMART; SM00667; LisH; 1.
DR PROSITE; PS50896; LISH; 1.
PE 1: Evidence at protein level;
KW Acetylation; Alternative splicing; DNA-binding; Nucleus; Phosphoprotein;
KW Reference proteome; Transcription; Transcription regulation.
FT CHAIN 1..361
FT /note="Single-stranded DNA-binding protein 2"
FT /id="PRO_0000123827"
FT DOMAIN 18..50
FT /note="LisH"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00126"
FT REGION 147..171
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 194..361
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 222..236
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 309..333
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 345..361
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 6
FT /note="N6-acetyllysine"
FT /evidence="ECO:0007744|PubMed:23806337"
FT MOD_RES 321
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT MOD_RES 333
FT /note="Phosphothreonine"
FT /evidence="ECO:0007744|PubMed:21183079"
FT VAR_SEQ 95..124
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_006259"
SQ SEQUENCE 361 AA; 37846 MW; EC6F98DCEBFF662E CRC64;
MYGKGKSNSS AVPSDSQARE KLALYVYEYL LHVGAQKSAQ TFLSEIRWEK NITLGEPPGF
LHSWWCVFWD LYCAAPERRE TCEHSSEAKA FHDYSAAAAP SPVLGNMPPG DGMPVGPVPP
GFFQPFMSPR YPGGPRPPLR IPNQALGGVP GSQPLLPSGM DPTRQQGHPN MGGPMQRMTP
PRGMVPLGPQ NYGGAMRPPL NALGGPGMPG MNMGPGGGRP WPNPTNANSI PYSSASPGNY
VGPPGGGGPP GTPIMPSPAD STNSGDNMYT LMNAVPPGPN RPNFPMGPGS DGPMGGLGGM
ESHHMNGSLG SGDMDSISKN SPNNMSLSNQ PGTPRDDGEM GGNFLNPFQS ESYSPSMTMS
V
