SSBPA_XENLA
ID SSBPA_XENLA Reviewed; 146 AA.
AC P09380; Q66GV9;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 2.
DT 03-AUG-2022, entry version 117.
DE RecName: Full=Single-stranded DNA-binding protein 1-A, mitochondrial;
DE AltName: Full=Single-stranded DNA-binding protein 1, mitochondrial;
DE Short=MtSSB-1;
DE Short=XlSSB1;
DE AltName: Full=Single-stranded DNA-binding protein S, mitochondrial;
DE Short=MtSSBs;
DE Short=xl-mtssb;
DE Flags: Precursor;
GN Name=ssbp1-a; Synonyms=mtssb, mtssb1;
OS Xenopus laevis (African clawed frog).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX NCBI_TaxID=8355;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC TISSUE=Ovary;
RX PubMed=1870981; DOI=10.1093/nar/19.15.4291;
RA Tiranti V., Barat-Gueride M., Bijl J., Didonato S., Zeviani M.;
RT "A full-length cDNA encoding a mitochondrial DNA-specific single-stranded
RT DNA binding protein from Xenopus laevis.";
RL Nucleic Acids Res. 19:4291-4291(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND DEVELOPMENTAL STAGE.
RX PubMed=9016954; DOI=10.1016/s0378-1119(96)00574-4;
RA Champagne A.M., Dufresne C., Viney L., Gueride M.;
RT "Cloning, sequencing and expression of the two genes encoding the
RT mitochondrial single-stranded DNA-binding protein in Xenopus laevis.";
RL Gene 184:65-71(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Testis;
RG NIH - Xenopus Gene Collection (XGC) project;
RL Submitted (SEP-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP PROTEIN SEQUENCE OF 18-142.
RX PubMed=1952953; DOI=10.1016/0003-9861(91)90152-9;
RA Ghrir R., Lecaer J.-P., Dufresne C., Barat-Gueride M.;
RT "Primary structure of the two variants of Xenopus laevis mtSSB, a
RT mitochondrial DNA binding protein.";
RL Arch. Biochem. Biophys. 291:395-400(1991).
RN [5]
RP PROTEIN SEQUENCE OF 18-41.
RX PubMed=3042458; DOI=10.1016/0014-5793(88)81276-6;
RA Mahoungou C., Ghrir R., Lecaer J.-P., Mignotte B., Barat-Gueride M.;
RT "The amino-terminal sequence of the Xenopus laevis mitochondrial SSB is
RT homologous to that of the Escherichia coli protein.";
RL FEBS Lett. 235:267-270(1988).
CC -!- FUNCTION: Binds preferentially and cooperatively to pyrimidine rich
CC single-stranded DNA (ss-DNA). Required to maintain the copy number of
CC mitochondrial DNA (mtDNA) and plays crucial roles during mtDNA
CC replication that stimulate activity of the DNA polymerase at the
CC replication fork. May also function in mtDNA repair.
CC {ECO:0000250|UniProtKB:Q04837}.
CC -!- SUBUNIT: Homotetramer. {ECO:0000305}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion {ECO:0000250|UniProtKB:Q04837}.
CC Mitochondrion matrix, mitochondrion nucleoid
CC {ECO:0000250|UniProtKB:Q04837}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=P09380-1; Sequence=Displayed;
CC Name=2;
CC IsoId=P09380-2; Sequence=VSP_036485;
CC -!- DEVELOPMENTAL STAGE: In oogenesis, expression is higher in
CC previtellogenic oocytes. {ECO:0000269|PubMed:9016954}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X59285; CAA41976.1; -; mRNA.
DR EMBL; X83673; CAA58647.1; -; Genomic_DNA.
DR EMBL; BC082205; AAH82205.1; -; mRNA.
DR PIR; JC6173; JC6173.
DR RefSeq; NP_001095241.1; NM_001101771.1. [P09380-1]
DR AlphaFoldDB; P09380; -.
DR SMR; P09380; -.
DR GeneID; 397915; -.
DR KEGG; xla:397915; -.
DR CTD; 397915; -.
DR Xenbase; XB-GENE-6252172; ssbp1.L.
DR Proteomes; UP000186698; Chromosome 3L.
DR Bgee; 397915; Expressed in oocyte and 20 other tissues.
DR GO; GO:0042645; C:mitochondrial nucleoid; ISS:UniProtKB.
DR GO; GO:0005739; C:mitochondrion; ISS:UniProtKB.
DR GO; GO:0003682; F:chromatin binding; ISS:UniProtKB.
DR GO; GO:0003697; F:single-stranded DNA binding; ISS:UniProtKB.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0090297; P:positive regulation of mitochondrial DNA replication; ISS:UniProtKB.
DR GO; GO:0051289; P:protein homotetramerization; ISS:UniProtKB.
DR CDD; cd04496; SSB_OBF; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR HAMAP; MF_00984; SSB; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR000424; Primosome_PriB/ssb.
DR InterPro; IPR011344; ssDNA-bd.
DR PANTHER; PTHR10302; PTHR10302; 1.
DR Pfam; PF00436; SSB; 1.
DR PIRSF; PIRSF002070; SSB; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR TIGRFAMs; TIGR00621; ssb; 1.
DR PROSITE; PS50935; SSB; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Direct protein sequencing; DNA replication;
KW DNA-binding; Mitochondrion; Mitochondrion nucleoid; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..17
FT /note="Mitochondrion"
FT /evidence="ECO:0000269|PubMed:1952953,
FT ECO:0000269|PubMed:3042458"
FT CHAIN 18..146
FT /note="Single-stranded DNA-binding protein 1-A,
FT mitochondrial"
FT /id="PRO_0000033266"
FT DOMAIN 28..140
FT /note="SSB"
FT VAR_SEQ 104
FT /note="G -> GAPEWNPVCPPPNSSPAVGPLALLASRRVSR (in isoform 2)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_036485"
SQ SEQUENCE 146 AA; 16743 MW; F97D77149A255B3E CRC64;
MFHRPALQVF RQFARCQSTD SVILERSINK VQLLGRVGQD PVMRQAEGKN PVTIFSLATN
ELWRSGESET FHTAGDVNQK TTWHRISVFR PGLRDVAYQH VKKGARLLVE GKIDYGEYTD
KNNVRRQATT IIADNIIFLS DLRDKL
