ID   SSB_BPLP2               Reviewed;         119 AA.
AC   Q6JM09;
DT   02-NOV-2016, integrated into UniProtKB/Swiss-Prot.
DT   20-APR-2010, sequence version 2.
DT   02-JUN-2021, entry version 25.
DE   RecName: Full=SSB protein {ECO:0000312|EMBL:AAR14301.2};
DE   AltName: Full=Gene product 34 {ECO:0000305};
DE            Short=Gp34 {ECO:0000305};
DE   AltName: Full=Single-stranded DNA binding protein {ECO:0000305};
OS   Lactococcus phage p2 (Lactococcus lactis bacteriophage p2).
OC   Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC   Caudovirales; Siphoviridae; Skunavirus.
OX   NCBI_TaxID=254252;
OH   NCBI_TaxID=1358; Lactococcus lactis.
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA   Tremblay D.M., Deveau H., Moineau S.;
RT   "Complete genomic sequence of Lactococcus lactis phage p2.";
RL   Submitted (FEB-2010) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   FUNCTION, DNA-BINDING, SUBUNIT, AND MUTAGENESIS OF ARG-16; LYS-22 AND
RP   LYS-25.
RX   PubMed=23429182; DOI=10.1016/j.bbapap.2013.02.014;
RA   Scaltriti E., Polverini E., Grolli S., Eufemi E., Moineau S., Cambillau C.,
RA   Ramoni R.;
RT   "The DNA binding mechanism of a SSB protein from Lactococcus lactis
RT   siphophage p2.";
RL   Biochim. Biophys. Acta 1834:1070-1076(2013).
RN   [3] {ECO:0007744|PDB:2WKC, ECO:0007744|PDB:2WKD}
RP   X-RAY CRYSTALLOGRAPHY (2.10 ANGSTROMS) OF 3-119, INDUCTION, FUNCTION,
RP   DNA-BINDING, AND SUBUNIT.
RX   PubMed=19719513; DOI=10.1111/j.1365-2958.2009.06844.x;
RA   Scaltriti E., Tegoni M., Rivetti C., Launay H., Masson J.Y., Magadan A.H.,
RA   Tremblay D., Moineau S., Ramoni R., Lichiere J., Campanacci V.,
RA   Cambillau C., Ortiz-Lombardia M.;
RT   "Structure and function of phage p2 ORF34(p2), a new type of single-
RT   stranded DNA binding protein.";
RL   Mol. Microbiol. 73:1156-1170(2009).
CC   -!- FUNCTION: Binds ssDNA with nanomolar affinity but no sequence
CC       specificity. {ECO:0000269|PubMed:19719513,
CC       ECO:0000269|PubMed:23429182}.
CC   -!- SUBUNIT: Homodimer; two homodimers can further weakly assemble into a
CC       homotetramer. {ECO:0000269|PubMed:19719513,
CC       ECO:0000269|PubMed:23429182}.
CC   -!- INDUCTION: Expressed in the early phase of the viral replicative cycle.
CC       {ECO:0000269|PubMed:19719513}.
CC   -!- SIMILARITY: Belongs to the skunalikevirus SSB protein family.
CC       {ECO:0000305}.
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DR   EMBL; GQ979703; AAR14301.2; -; Genomic_DNA.
DR   PDB; 2WKC; X-ray; 2.60 A; A/B/C/D=3-119.
DR   PDB; 2WKD; X-ray; 2.10 A; A=3-119.
DR   PDBsum; 2WKC; -.
DR   PDBsum; 2WKD; -.
DR   SMR; Q6JM09; -.
DR   Proteomes; UP000002348; Genome.
DR   GO; GO:0003697; F:single-stranded DNA binding; IDA:UniProtKB.
DR   Gene3D; 2.40.50.400; -; 1.
DR   InterPro; IPR038621; Lacto_phage_SSB_sf.
DR   InterPro; IPR031900; Phage_SSB.
DR   Pfam; PF16773; Phage_SSB; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Early protein; Reference proteome.
FT   CHAIN           1..119
FT                   /note="SSB protein"
FT                   /id="PRO_0000438234"
FT   REGION          96..119
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MUTAGEN         16
FT                   /note="R->A: Loss of ssDNA-binding."
FT                   /evidence="ECO:0000269|PubMed:23429182"
FT   MUTAGEN         22
FT                   /note="K->A: No effect on ssDNA-binding."
FT                   /evidence="ECO:0000269|PubMed:23429182"
FT   MUTAGEN         25
FT                   /note="K->A: No effect on ssDNA-binding."
FT                   /evidence="ECO:0000269|PubMed:23429182"
SQ   SEQUENCE   119 AA;  12970 MW;  FD347A46CE235E46 CRC64;
     MAIITVTAQA NEKNTRTVST AKGDKKIISV PLFEKEKGSN VKVAYGSAFL PDFIQLGDTV
     TVSGRVQAKE SGEYVNYNFV FPTVEKVFIT NDNSSQSQAK QDLFGGSEPI EVNSEDLPF