SSB_BPNF
ID SSB_BPNF Reviewed; 122 AA.
AC Q9MCD1;
DT 11-MAY-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 29-SEP-2021, entry version 46.
DE RecName: Full=Single-stranded DNA-binding protein;
DE Short=SSB;
DE AltName: Full=Gene product 5;
DE Short=gp5;
DE AltName: Full=Protein p5;
GN Name=5;
OS Bacillus phage Nf (Bacteriophage Nf).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Salasmaviridae; Picovirinae; Beecentumtrevirus.
OX NCBI_TaxID=10753 {ECO:0000312|EMBL:CAB91843.1};
OH NCBI_TaxID=1423; Bacillus subtilis.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RX PubMed=10773070; DOI=10.1093/nar/28.10.2034;
RA Gascon I., Lazaro J.M., Salas M.;
RT "Differential functional behavior of viral phi29, Nf and GA-1 SSB
RT proteins.";
RL Nucleic Acids Res. 28:2034-2042(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Castilla-Llorente V., Salas M., Meijer W.J.J.;
RT "Different responses to Spo0A-mediated suppression of the related Bacillus
RT subtilis phages Nf and phi29.";
RL Environ. Microbiol. 11:1137-1149(2009).
RN [3]
RP SUBUNIT, AND DNA-BINDING.
RX PubMed=10686098; DOI=10.1006/jmbi.2000.3521;
RA Gascon I., Gutierrez C., Salas M.;
RT "Structural and functional comparative study of the complexes formed by
RT viral phi29, Nf and GA-1 SSB proteins with DNA.";
RL J. Mol. Biol. 296:989-999(2000).
CC -!- FUNCTION: Single-stranded DNA-binding protein required for the
CC elongation during viral DNA replication by strand displacement.
CC Displaced viral DNA strands are transiently coated with the ssDNA-
CC binding protein and therefore protected againt nucleases. The latter is
CC then probably removed by the replisome that performs lagging strand
CC synthesis or during the events that lead up to the recombination
CC process. Has helix-destabilizing activity since it removes secondary
CC structure from the ssDNA in replicative intermediates.
CC {ECO:0000305|PubMed:10773070}.
CC -!- SUBUNIT: Monomer. Binds to DNA. {ECO:0000269|PubMed:10686098}.
CC -!- SIMILARITY: Belongs to the phi29likevirus single-strand-binding protein
CC family. {ECO:0000305}.
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DR EMBL; EU622808; ACH57072.1; -; Genomic_DNA.
DR EMBL; AJ244025; CAB91843.1; -; Genomic_DNA.
DR Proteomes; UP000000744; Genome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-KW.
DR GO; GO:0039693; P:viral DNA genome replication; IEA:UniProtKB-KW.
DR InterPro; IPR035408; Phi29_Phage_SSB.
DR Pfam; PF17427; Phi29_Phage_SSB; 1.
PE 1: Evidence at protein level;
KW DNA replication; DNA-binding; Early protein; Reference proteome;
KW Viral DNA replication.
FT CHAIN 1..122
FT /note="Single-stranded DNA-binding protein"
FT /id="PRO_0000436075"
SQ SEQUENCE 122 AA; 13090 MW; 98820B89FC906D2F CRC64;
MTNEIKATFD VTTLEGRMKI LNAKNAGGAS LKTCEDGAII EAVGIAQYQQ ESDTYGDMKE
ETVTAIFTAD GNVISAISKT VAEAASEIID LVKEFNLDSF KVKVSKQKSS KGNEFFSLLL
VG