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SSB_DEIRA
ID   SSB_DEIRA               Reviewed;         301 AA.
AC   Q9RY51;
DT   03-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2003, sequence version 2.
DT   25-MAY-2022, entry version 135.
DE   RecName: Full=Single-stranded DNA-binding protein {ECO:0000255|HAMAP-Rule:MF_00984};
DE            Short=SSB {ECO:0000255|HAMAP-Rule:MF_00984};
GN   Name=ssb; OrderedLocusNames=DR_0099;
OS   Deinococcus radiodurans (strain ATCC 13939 / DSM 20539 / JCM 16871 / LMG
OS   4051 / NBRC 15346 / NCIMB 9279 / R1 / VKM B-1422).
OC   Bacteria; Deinococcus-Thermus; Deinococci; Deinococcales; Deinococcaceae;
OC   Deinococcus.
OX   NCBI_TaxID=243230;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBUNIT.
RC   STRAIN=ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB
RC   9279 / R1 / VKM B-1422;
RX   PubMed=14718065; DOI=10.1186/1471-2180-4-2;
RA   Eggington J.M., Haruta N., Wood E.A., Cox M.M.;
RT   "The single-stranded DNA-binding protein of Deinococcus radiodurans.";
RL   BMC Microbiol. 4:2-2(2004).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBUNIT, AND DNA-BINDING.
RC   STRAIN=ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB
RC   9279 / R1 / VKM B-1422;
RX   PubMed=15781492; DOI=10.1093/nar/gki310;
RA   Witte G., Urbanke C., Curth U.;
RT   "Single-stranded DNA-binding protein of Deinococcus radiodurans: a
RT   biophysical characterization.";
RL   Nucleic Acids Res. 33:1662-1670(2005).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB
RC   9279 / R1 / VKM B-1422;
RX   PubMed=10567266; DOI=10.1126/science.286.5444.1571;
RA   White O., Eisen J.A., Heidelberg J.F., Hickey E.K., Peterson J.D.,
RA   Dodson R.J., Haft D.H., Gwinn M.L., Nelson W.C., Richardson D.L.,
RA   Moffat K.S., Qin H., Jiang L., Pamphile W., Crosby M., Shen M.,
RA   Vamathevan J.J., Lam P., McDonald L.A., Utterback T.R., Zalewski C.,
RA   Makarova K.S., Aravind L., Daly M.J., Minton K.W., Fleischmann R.D.,
RA   Ketchum K.A., Nelson K.E., Salzberg S.L., Smith H.O., Venter J.C.,
RA   Fraser C.M.;
RT   "Genome sequence of the radioresistant bacterium Deinococcus radiodurans
RT   R1.";
RL   Science 286:1571-1577(1999).
RN   [4]
RP   FUNCTION AS AN SSB PROTEIN, AND DNA-BINDING.
RC   STRAIN=ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB
RC   9279 / R1 / VKM B-1422;
RX   PubMed=15466873; DOI=10.1074/jbc.m408645200;
RA   Wang J., Julin D.A.;
RT   "DNA helicase activity of the RecD protein from Deinococcus radiodurans.";
RL   J. Biol. Chem. 279:52024-52032(2004).
RN   [5]
RP   FUNCTION, AND INTERACTION WITH DDRB.
RC   STRAIN=ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB
RC   9279 / R1 / VKM B-1422;
RX   PubMed=20451472; DOI=10.1016/j.dnarep.2010.04.006;
RA   Xu G., Lu H., Wang L., Chen H., Xu Z., Hu Y., Tian B., Hua Y.;
RT   "DdrB stimulates single-stranded DNA annealing and facilitates RecA-
RT   independent DNA repair in Deinococcus radiodurans.";
RL   DNA Repair 9:805-812(2010).
RN   [6]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB
RC   9279 / R1 / VKM B-1422;
RX   PubMed=23951213; DOI=10.1371/journal.pone.0071651;
RA   Lockhart J.S., DeVeaux L.C.;
RT   "The essential role of the Deinococcus radiodurans ssb gene in cell
RT   survival and radiation tolerance.";
RL   PLoS ONE 8:E71651-E71651(2013).
RN   [7]
RP   X-RAY CRYSTALLOGRAPHY (1.80 ANGSTROMS), AND SUBUNIT.
RC   STRAIN=ATCC 13939 / DSM 20539 / JCM 16871 / LMG 4051 / NBRC 15346 / NCIMB
RC   9279 / R1 / VKM B-1422;
RX   PubMed=15159541; DOI=10.1073/pnas.0401331101;
RA   Bernstein D.A., Eggington J.M., Killoran M.P., Misic A.M., Cox M.M.,
RA   Keck J.L.;
RT   "Crystal structure of the Deinococcus radiodurans single-stranded DNA-
RT   binding protein suggests a mechanism for coping with DNA damage.";
RL   Proc. Natl. Acad. Sci. U.S.A. 101:8575-8580(2004).
RN   [8]
RP   X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF 290-301.
RA   George N.P., Keck J.L.;
RT   "Identification of the SSB-interaction platform of Deinococcus radiodurans
RT   uracil-DNA glycosylase.";
RL   Submitted (NOV-2011) to the PDB data bank.
RN   [9]
RP   X-RAY CRYSTALLOGRAPHY (2.40 ANGSTROMS) IN COMPLEX WITH SSDNA, SUBUNIT,
RP   SUBCELLULAR LOCATION, AND INDUCTION.
RX   PubMed=22570477; DOI=10.1074/jbc.m112.367573;
RA   George N.P., Ngo K.V., Chitteni-Pattu S., Norais C.A., Battista J.R.,
RA   Cox M.M., Keck J.L.;
RT   "Structure and cellular dynamics of Deinococcus radiodurans single-stranded
RT   DNA (ssDNA)-binding protein (SSB)-DNA complexes.";
RL   J. Biol. Chem. 287:22123-22132(2012).
CC   -!- FUNCTION: Plays an important role in DNA replication, recombination and
CC       repair. Binds to ssDNA and to an array of partner proteins to recruit
CC       them to their sites of action during DNA metabolism (By similarity).
CC       Essential for ionizing radiation resistance. Stimulates the 5'-3' DNA
CC       helicase activity of RecD-like helicase. Stimulates RecA protein-
CC       promoted DNA three-strand exchange reactions in vitro with both
CC       D.radiodurans and E.coli-derived RecA. Complements an ssb deletion in
CC       E.coli, but does not complement a ddrb disruption in D.radiodurans.
CC       {ECO:0000255|HAMAP-Rule:MF_00984, ECO:0000269|PubMed:14718065,
CC       ECO:0000269|PubMed:15466873, ECO:0000269|PubMed:20451472,
CC       ECO:0000269|PubMed:23951213}.
CC   -!- SUBUNIT: Homodimer. Binds ssDNA. Interacts with DdrB.
CC       {ECO:0000269|PubMed:14718065, ECO:0000269|PubMed:15159541,
CC       ECO:0000269|PubMed:15781492, ECO:0000269|PubMed:20451472,
CC       ECO:0000269|PubMed:22570477}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:22570477}.
CC       Note=During log phase, present in multiple foci. 1 hour after
CC       irradiation most foci are condensed in the center of the cell's
CC       nucleoid, they dissipate by 2 hours later.
CC   -!- INDUCTION: Constitutively expressed, highly up-regulated following
CC       ionizing radiation for at least 12 hours (at protein level).
CC       {ECO:0000269|PubMed:22570477}.
CC   -!- DISRUPTION PHENOTYPE: Essential, it cannot be knocked out. As SSB
CC       levels are depleted growth slows, tolerance to ionizing radiation and
CC       UV light decreases rapidly. Cannot be complemented by ddrB.
CC       {ECO:0000269|PubMed:23951213}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAF09692.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; AY293617; AAQ18705.1; -; Genomic_DNA.
DR   EMBL; AJ564860; CAD92322.1; -; Genomic_DNA.
DR   EMBL; AE000513; AAF09692.1; ALT_FRAME; Genomic_DNA.
DR   PIR; A75559; A75559.
DR   RefSeq; NP_293825.1; NC_001263.1.
DR   RefSeq; WP_027480308.1; NZ_CP015081.1.
DR   PDB; 1SE8; X-ray; 1.80 A; A=1-301.
DR   PDB; 3UDG; X-ray; 2.40 A; A/B/C=1-301.
DR   PDB; 3UFM; X-ray; 1.95 A; B=290-301.
DR   PDBsum; 1SE8; -.
DR   PDBsum; 3UDG; -.
DR   PDBsum; 3UFM; -.
DR   AlphaFoldDB; Q9RY51; -.
DR   SMR; Q9RY51; -.
DR   STRING; 243230.DR_0099; -.
DR   EnsemblBacteria; AAF09692; AAF09692; DR_0099.
DR   KEGG; dra:DR_0099; -.
DR   PATRIC; fig|243230.17.peg.263; -.
DR   eggNOG; COG0629; Bacteria.
DR   HOGENOM; CLU_1802971_0_0_0; -.
DR   InParanoid; Q9RY51; -.
DR   OMA; ARGMNHV; -.
DR   OrthoDB; 1942216at2; -.
DR   EvolutionaryTrace; Q9RY51; -.
DR   Proteomes; UP000002524; Chromosome I.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0009295; C:nucleoid; IBA:GO_Central.
DR   GO; GO:0003697; F:single-stranded DNA binding; IBA:GO_Central.
DR   GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR   GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR   GO; GO:0051096; P:positive regulation of helicase activity; IBA:GO_Central.
DR   CDD; cd04496; SSB_OBF; 2.
DR   Gene3D; 2.40.50.140; -; 2.
DR   HAMAP; MF_00984; SSB; 1.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR000424; Primosome_PriB/ssb.
DR   InterPro; IPR011344; ssDNA-bd.
DR   PANTHER; PTHR10302; PTHR10302; 2.
DR   Pfam; PF00436; SSB; 2.
DR   SUPFAM; SSF50249; SSF50249; 2.
DR   TIGRFAMs; TIGR00621; ssb; 2.
DR   PROSITE; PS50935; SSB; 2.
PE   1: Evidence at protein level;
KW   3D-structure; Cytoplasm; DNA damage; DNA recombination; DNA repair;
KW   DNA replication; DNA-binding; Reference proteome; Repeat.
FT   CHAIN           1..301
FT                   /note="Single-stranded DNA-binding protein"
FT                   /id="PRO_0000096147"
FT   DOMAIN          5..110
FT                   /note="SSB 1"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00984"
FT   DOMAIN          129..232
FT                   /note="SSB 2"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00984"
FT   REGION          238..301
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           296..301
FT                   /note="Important for interaction with partner proteins"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00984"
FT   COMPBIAS        246..282
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   STRAND          5..16
FT                   /evidence="ECO:0007829|PDB:1SE8"
FT   STRAND          19..22
FT                   /evidence="ECO:0007829|PDB:1SE8"
FT   STRAND          28..39
FT                   /evidence="ECO:0007829|PDB:1SE8"
FT   STRAND          49..60
FT                   /evidence="ECO:0007829|PDB:1SE8"
FT   HELIX           61..68
FT                   /evidence="ECO:0007829|PDB:1SE8"
FT   STRAND          76..88
FT                   /evidence="ECO:0007829|PDB:1SE8"
FT   STRAND          96..108
FT                   /evidence="ECO:0007829|PDB:1SE8"
FT   STRAND          115..117
FT                   /evidence="ECO:0007829|PDB:1SE8"
FT   STRAND          123..126
FT                   /evidence="ECO:0007829|PDB:1SE8"
FT   STRAND          129..138
FT                   /evidence="ECO:0007829|PDB:1SE8"
FT   STRAND          143..146
FT                   /evidence="ECO:0007829|PDB:1SE8"
FT   STRAND          152..165
FT                   /evidence="ECO:0007829|PDB:1SE8"
FT   STRAND          171..183
FT                   /evidence="ECO:0007829|PDB:1SE8"
FT   HELIX           185..190
FT                   /evidence="ECO:0007829|PDB:1SE8"
FT   STRAND          198..209
FT                   /evidence="ECO:0007829|PDB:1SE8"
FT   STRAND          219..230
FT                   /evidence="ECO:0007829|PDB:1SE8"
SQ   SEQUENCE   301 AA;  32722 MW;  21B2090C970E1B0E CRC64;
     MARGMNHVYL IGALARDPEL RYTGNGMAVF EATVAGEDRV IGNDGRERNL PWYHRVSILG
     KPAEWQAERN LKGGDAVVVE GTLEYRQWEA PEGGKRSAVN VKALRMEQLG TQPELIQDAG
     GGVRMSGAMN EVLVLGNVTR DPEIRYTPAG DAVLSLSIAV NENYQDRQGQ RQEKVHYIDA
     TLWRDLAENM KELRKGDPVM IMGRLVNEGW TDKDGNKRNS TRVEATRVEA LARGAGNANS
     GYAAATPAAP RTQTASSAAR PTSGGYQSQP SRAANTGSRS GGLDIDQGLD DFPPEEDDLP
     F
 
 
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