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SSB_MYCS2
ID   SSB_MYCS2               Reviewed;         165 AA.
AC   Q9AFI5; A0R7F8; I7FVY6;
DT   24-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   25-MAY-2022, entry version 129.
DE   RecName: Full=Single-stranded DNA-binding protein {ECO:0000255|HAMAP-Rule:MF_00984};
DE            Short=SSB {ECO:0000255|HAMAP-Rule:MF_00984};
GN   Name=ssb; OrderedLocusNames=MSMEG_6896, MSMEI_6712;
OS   Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS   smegmatis).
OC   Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC   Mycolicibacterium.
OX   NCBI_TaxID=246196;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INTERACTION WITH RECA.
RX   PubMed=11577073; DOI=10.1074/jbc.m103523200;
RA   Reddy M.S., Guhan N., Muniyappa K.;
RT   "Characterization of single-stranded DNA-binding proteins from
RT   Mycobacteria. The carboxyl-terminal of domain of SSB is essential for
RT   stable association with its cognate RecA protein.";
RL   J. Biol. Chem. 276:45959-45968(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RA   Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA   Fraser C.M.;
RL   Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA   Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA   Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT   "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT   mutations or sequencing errors?";
RL   Genome Biol. 8:R20.1-R20.9(2007).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700084 / mc(2)155;
RX   PubMed=18955433; DOI=10.1101/gr.081901.108;
RA   Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA   Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT   "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT   and a new MS-based protocol.";
RL   Genome Res. 19:128-135(2009).
RN   [5]
RP   X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS), AND SUBUNIT.
RX   PubMed=16041080; DOI=10.1107/s0907444905016896;
RA   Saikrishnan K., Manjunath G.P., Singh P., Jeyakanthan J., Dauter Z.,
RA   Sekar K., Muniyappa K., Vijayan M.;
RT   "Structure of Mycobacterium smegmatis single-stranded DNA-binding protein
RT   and a comparative study involving homologous SSBs: biological implications
RT   of structural plasticity and variability in quaternary association.";
RL   Acta Crystallogr. D 61:1140-1148(2005).
RN   [6]
RP   X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) OF 1-130 IN COMPLEX WITH SSDNA.
RA   Kaushal P.S., Manjunath G.P., Sekar K., Muniyappa K., Vijayan M.;
RT   "Promiscuity and specificity in DNA binding to SSB: Insights from the
RT   structure of the Mycobacterium smegmatis SSB-ssDNA complex.";
RL   Submitted (AUG-2009) to the PDB data bank.
CC   -!- SUBUNIT: Homotetramer. Interacts with RecA. {ECO:0000255|HAMAP-
CC       Rule:MF_00984, ECO:0000269|PubMed:11577073,
CC       ECO:0000269|PubMed:16041080, ECO:0000269|Ref.6}.
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DR   EMBL; AF349434; AAK30583.1; -; Genomic_DNA.
DR   EMBL; CP000480; ABK71618.1; -; Genomic_DNA.
DR   EMBL; CP001663; AFP43138.1; -; Genomic_DNA.
DR   RefSeq; WP_003898320.1; NZ_SIJM01000001.1.
DR   RefSeq; YP_891096.1; NC_008596.1.
DR   PDB; 1X3E; X-ray; 2.15 A; A/B=1-165.
DR   PDB; 1X3F; X-ray; 2.70 A; A/B=1-165.
DR   PDB; 1X3G; X-ray; 3.00 A; A/B=1-165.
DR   PDB; 3A5U; X-ray; 2.80 A; A/B=1-130.
DR   PDBsum; 1X3E; -.
DR   PDBsum; 1X3F; -.
DR   PDBsum; 1X3G; -.
DR   PDBsum; 3A5U; -.
DR   AlphaFoldDB; Q9AFI5; -.
DR   SMR; Q9AFI5; -.
DR   STRING; 246196.MSMEI_6712; -.
DR   PRIDE; Q9AFI5; -.
DR   EnsemblBacteria; ABK71618; ABK71618; MSMEG_6896.
DR   EnsemblBacteria; AFP43138; AFP43138; MSMEI_6712.
DR   GeneID; 66738150; -.
DR   KEGG; msg:MSMEI_6712; -.
DR   KEGG; msm:MSMEG_6896; -.
DR   PATRIC; fig|246196.19.peg.6717; -.
DR   eggNOG; COG0629; Bacteria.
DR   OMA; GQMQERT; -.
DR   OrthoDB; 1942216at2; -.
DR   EvolutionaryTrace; Q9AFI5; -.
DR   Proteomes; UP000000757; Chromosome.
DR   Proteomes; UP000006158; Chromosome.
DR   GO; GO:0003697; F:single-stranded DNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR   CDD; cd04496; SSB_OBF; 1.
DR   Gene3D; 2.40.50.140; -; 1.
DR   HAMAP; MF_00984; SSB; 1.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR000424; Primosome_PriB/ssb.
DR   InterPro; IPR011344; ssDNA-bd.
DR   PANTHER; PTHR10302; PTHR10302; 1.
DR   Pfam; PF00436; SSB; 1.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   TIGRFAMs; TIGR00621; ssb; 1.
DR   PROSITE; PS50935; SSB; 1.
PE   1: Evidence at protein level;
KW   3D-structure; DNA-binding; Reference proteome.
FT   CHAIN           1..165
FT                   /note="Single-stranded DNA-binding protein"
FT                   /id="PRO_0000096068"
FT   DOMAIN          1..110
FT                   /note="SSB"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00984"
FT   REGION          117..165
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   STRAND          6..15
FT                   /evidence="ECO:0007829|PDB:1X3E"
FT   STRAND          18..21
FT                   /evidence="ECO:0007829|PDB:1X3E"
FT   STRAND          27..34
FT                   /evidence="ECO:0007829|PDB:1X3E"
FT   STRAND          42..44
FT                   /evidence="ECO:0007829|PDB:1X3F"
FT   STRAND          54..60
FT                   /evidence="ECO:0007829|PDB:1X3E"
FT   HELIX           62..70
FT                   /evidence="ECO:0007829|PDB:1X3E"
FT   STRAND          76..89
FT                   /evidence="ECO:0007829|PDB:1X3E"
FT   STRAND          95..108
FT                   /evidence="ECO:0007829|PDB:1X3E"
FT   STRAND          110..119
FT                   /evidence="ECO:0007829|PDB:1X3E"
SQ   SEQUENCE   165 AA;  17401 MW;  8786415C16F26F39 CRC64;
     MAGDTTITVV GNLTADPELR FTPSGAAVAN FTVASTPRMF DRQSGEWKDG EALFLRCNIW
     REAAENVAES LTRGSRVIVT GRLKQRSFET REGEKRTVVE VEVDEIGPSL RYATAKVNKA
     SRSGGGGGGF GSGGGGSRQS EPKDDPWGSA PASGSFSGAD DEPPF
 
 
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