SSB_MYCS2
ID SSB_MYCS2 Reviewed; 165 AA.
AC Q9AFI5; A0R7F8; I7FVY6;
DT 24-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 1.
DT 25-MAY-2022, entry version 129.
DE RecName: Full=Single-stranded DNA-binding protein {ECO:0000255|HAMAP-Rule:MF_00984};
DE Short=SSB {ECO:0000255|HAMAP-Rule:MF_00984};
GN Name=ssb; OrderedLocusNames=MSMEG_6896, MSMEI_6712;
OS Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) (Mycobacterium
OS smegmatis).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycolicibacterium.
OX NCBI_TaxID=246196;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INTERACTION WITH RECA.
RX PubMed=11577073; DOI=10.1074/jbc.m103523200;
RA Reddy M.S., Guhan N., Muniyappa K.;
RT "Characterization of single-stranded DNA-binding proteins from
RT Mycobacteria. The carboxyl-terminal of domain of SSB is essential for
RT stable association with its cognate RecA protein.";
RL J. Biol. Chem. 276:45959-45968(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RA Fleischmann R.D., Dodson R.J., Haft D.H., Merkel J.S., Nelson W.C.,
RA Fraser C.M.;
RL Submitted (OCT-2006) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=17295914; DOI=10.1186/gb-2007-8-2-r20;
RA Deshayes C., Perrodou E., Gallien S., Euphrasie D., Schaeffer C.,
RA Van-Dorsselaer A., Poch O., Lecompte O., Reyrat J.-M.;
RT "Interrupted coding sequences in Mycobacterium smegmatis: authentic
RT mutations or sequencing errors?";
RL Genome Biol. 8:R20.1-R20.9(2007).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700084 / mc(2)155;
RX PubMed=18955433; DOI=10.1101/gr.081901.108;
RA Gallien S., Perrodou E., Carapito C., Deshayes C., Reyrat J.-M.,
RA Van Dorsselaer A., Poch O., Schaeffer C., Lecompte O.;
RT "Ortho-proteogenomics: multiple proteomes investigation through orthology
RT and a new MS-based protocol.";
RL Genome Res. 19:128-135(2009).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.15 ANGSTROMS), AND SUBUNIT.
RX PubMed=16041080; DOI=10.1107/s0907444905016896;
RA Saikrishnan K., Manjunath G.P., Singh P., Jeyakanthan J., Dauter Z.,
RA Sekar K., Muniyappa K., Vijayan M.;
RT "Structure of Mycobacterium smegmatis single-stranded DNA-binding protein
RT and a comparative study involving homologous SSBs: biological implications
RT of structural plasticity and variability in quaternary association.";
RL Acta Crystallogr. D 61:1140-1148(2005).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (2.80 ANGSTROMS) OF 1-130 IN COMPLEX WITH SSDNA.
RA Kaushal P.S., Manjunath G.P., Sekar K., Muniyappa K., Vijayan M.;
RT "Promiscuity and specificity in DNA binding to SSB: Insights from the
RT structure of the Mycobacterium smegmatis SSB-ssDNA complex.";
RL Submitted (AUG-2009) to the PDB data bank.
CC -!- SUBUNIT: Homotetramer. Interacts with RecA. {ECO:0000255|HAMAP-
CC Rule:MF_00984, ECO:0000269|PubMed:11577073,
CC ECO:0000269|PubMed:16041080, ECO:0000269|Ref.6}.
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DR EMBL; AF349434; AAK30583.1; -; Genomic_DNA.
DR EMBL; CP000480; ABK71618.1; -; Genomic_DNA.
DR EMBL; CP001663; AFP43138.1; -; Genomic_DNA.
DR RefSeq; WP_003898320.1; NZ_SIJM01000001.1.
DR RefSeq; YP_891096.1; NC_008596.1.
DR PDB; 1X3E; X-ray; 2.15 A; A/B=1-165.
DR PDB; 1X3F; X-ray; 2.70 A; A/B=1-165.
DR PDB; 1X3G; X-ray; 3.00 A; A/B=1-165.
DR PDB; 3A5U; X-ray; 2.80 A; A/B=1-130.
DR PDBsum; 1X3E; -.
DR PDBsum; 1X3F; -.
DR PDBsum; 1X3G; -.
DR PDBsum; 3A5U; -.
DR AlphaFoldDB; Q9AFI5; -.
DR SMR; Q9AFI5; -.
DR STRING; 246196.MSMEI_6712; -.
DR PRIDE; Q9AFI5; -.
DR EnsemblBacteria; ABK71618; ABK71618; MSMEG_6896.
DR EnsemblBacteria; AFP43138; AFP43138; MSMEI_6712.
DR GeneID; 66738150; -.
DR KEGG; msg:MSMEI_6712; -.
DR KEGG; msm:MSMEG_6896; -.
DR PATRIC; fig|246196.19.peg.6717; -.
DR eggNOG; COG0629; Bacteria.
DR OMA; GQMQERT; -.
DR OrthoDB; 1942216at2; -.
DR EvolutionaryTrace; Q9AFI5; -.
DR Proteomes; UP000000757; Chromosome.
DR Proteomes; UP000006158; Chromosome.
DR GO; GO:0003697; F:single-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR CDD; cd04496; SSB_OBF; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR HAMAP; MF_00984; SSB; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR000424; Primosome_PriB/ssb.
DR InterPro; IPR011344; ssDNA-bd.
DR PANTHER; PTHR10302; PTHR10302; 1.
DR Pfam; PF00436; SSB; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR TIGRFAMs; TIGR00621; ssb; 1.
DR PROSITE; PS50935; SSB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Reference proteome.
FT CHAIN 1..165
FT /note="Single-stranded DNA-binding protein"
FT /id="PRO_0000096068"
FT DOMAIN 1..110
FT /note="SSB"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00984"
FT REGION 117..165
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT STRAND 6..15
FT /evidence="ECO:0007829|PDB:1X3E"
FT STRAND 18..21
FT /evidence="ECO:0007829|PDB:1X3E"
FT STRAND 27..34
FT /evidence="ECO:0007829|PDB:1X3E"
FT STRAND 42..44
FT /evidence="ECO:0007829|PDB:1X3F"
FT STRAND 54..60
FT /evidence="ECO:0007829|PDB:1X3E"
FT HELIX 62..70
FT /evidence="ECO:0007829|PDB:1X3E"
FT STRAND 76..89
FT /evidence="ECO:0007829|PDB:1X3E"
FT STRAND 95..108
FT /evidence="ECO:0007829|PDB:1X3E"
FT STRAND 110..119
FT /evidence="ECO:0007829|PDB:1X3E"
SQ SEQUENCE 165 AA; 17401 MW; 8786415C16F26F39 CRC64;
MAGDTTITVV GNLTADPELR FTPSGAAVAN FTVASTPRMF DRQSGEWKDG EALFLRCNIW
REAAENVAES LTRGSRVIVT GRLKQRSFET REGEKRTVVE VEVDEIGPSL RYATAKVNKA
SRSGGGGGGF GSGGGGSRQS EPKDDPWGSA PASGSFSGAD DEPPF