SSB_MYCTU
ID SSB_MYCTU Reviewed; 164 AA.
AC P9WGD5; L0T5J5; P0A610; P71711;
DT 16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT 16-APR-2014, sequence version 1.
DT 25-MAY-2022, entry version 42.
DE RecName: Full=Single-stranded DNA-binding protein {ECO:0000255|HAMAP-Rule:MF_00984};
DE Short=SSB {ECO:0000255|HAMAP-Rule:MF_00984};
GN Name=ssb; OrderedLocusNames=Rv0054; ORFNames=MTCY21D4.17;
OS Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv).
OC Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae;
OC Mycobacterium; Mycobacterium tuberculosis complex.
OX NCBI_TaxID=83332;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=9634230; DOI=10.1038/31159;
RA Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E.,
RA Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K.,
RA Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K.,
RA Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K.,
RA Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J.,
RA Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S.,
RA Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S.,
RA Barrell B.G.;
RT "Deciphering the biology of Mycobacterium tuberculosis from the complete
RT genome sequence.";
RL Nature 393:537-544(1998).
RN [2]
RP CHARACTERIZATION.
RX PubMed=10491108; DOI=10.1046/j.1432-1327.1999.00684.x;
RA Purnapatre K., Varshney U.;
RT "Cloning, over-expression and biochemical characterization of the single-
RT stranded DNA binding protein from Mycobacterium tuberculosis.";
RL Eur. J. Biochem. 264:591-598(1999).
RN [3]
RP SSDNA-BINDING.
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=20724443; DOI=10.1093/nar/gkq737;
RA Huang F., He Z.G.;
RT "Characterization of an interplay between a Mycobacterium tuberculosis MazF
RT homolog, Rv1495 and its sole DNA topoisomerase I.";
RL Nucleic Acids Res. 38:8219-8230(2010).
RN [4]
RP CRYSTALLIZATION, AND SUBUNIT.
RX PubMed=12888346; DOI=10.1016/s0022-2836(03)00729-0;
RA Saikrishnan K., Jeyakanthan J., Venkatesh J., Acharya N., Sekar K.,
RA Varshney U., Vijayan M.;
RT "Structure of Mycobacterium tuberculosis single-stranded DNA-binding
RT protein. Variability in quaternary structure and its implications.";
RL J. Mol. Biol. 331:385-393(2003).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC STRAIN=ATCC 25618 / H37Rv;
RX PubMed=21969609; DOI=10.1074/mcp.m111.011627;
RA Kelkar D.S., Kumar D., Kumar P., Balakrishnan L., Muthusamy B., Yadav A.K.,
RA Shrivastava P., Marimuthu A., Anand S., Sundaram H., Kingsbury R.,
RA Harsha H.C., Nair B., Prasad T.S., Chauhan D.S., Katoch K., Katoch V.M.,
RA Kumar P., Chaerkady R., Ramachandran S., Dash D., Pandey A.;
RT "Proteogenomic analysis of Mycobacterium tuberculosis by high resolution
RT mass spectrometry.";
RL Mol. Cell. Proteomics 10:M111.011627-M111.011627(2011).
CC -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_00984,
CC ECO:0000269|PubMed:12888346}.
CC -!- INTERACTION:
CC P9WGD5; P9WMR3: dnaB; NbExp=3; IntAct=EBI-9081374, EBI-9081402;
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DR EMBL; AL123456; CCP42776.1; -; Genomic_DNA.
DR PIR; F70913; F70913.
DR RefSeq; NP_214568.1; NC_000962.3.
DR RefSeq; WP_003400534.1; NZ_NVQJ01000005.1.
DR PDB; 1UE1; X-ray; 2.50 A; A/B=1-164.
DR PDB; 1UE5; X-ray; 2.60 A; A/B=1-164.
DR PDB; 1UE6; X-ray; 2.70 A; A/B/C/D=1-164.
DR PDB; 1UE7; X-ray; 3.20 A; A/B/C/D=1-164.
DR PDBsum; 1UE1; -.
DR PDBsum; 1UE5; -.
DR PDBsum; 1UE6; -.
DR PDBsum; 1UE7; -.
DR AlphaFoldDB; P9WGD5; -.
DR SMR; P9WGD5; -.
DR IntAct; P9WGD5; 1.
DR MINT; P9WGD5; -.
DR STRING; 83332.Rv0054; -.
DR PaxDb; P9WGD5; -.
DR DNASU; 887013; -.
DR GeneID; 887013; -.
DR KEGG; mtu:Rv0054; -.
DR TubercuList; Rv0054; -.
DR eggNOG; COG0629; Bacteria.
DR OMA; GQMQERT; -.
DR PhylomeDB; P9WGD5; -.
DR Proteomes; UP000001584; Chromosome.
DR GO; GO:0005576; C:extracellular region; HDA:MTBBASE.
DR GO; GO:0009295; C:nucleoid; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; HDA:MTBBASE.
DR GO; GO:0003697; F:single-stranded DNA binding; IDA:MTBBASE.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IEP:MTBBASE.
DR GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR GO; GO:0051096; P:positive regulation of helicase activity; IBA:GO_Central.
DR GO; GO:0046677; P:response to antibiotic; IEP:MTBBASE.
DR CDD; cd04496; SSB_OBF; 1.
DR Gene3D; 2.40.50.140; -; 1.
DR HAMAP; MF_00984; SSB; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR000424; Primosome_PriB/ssb.
DR InterPro; IPR011344; ssDNA-bd.
DR PANTHER; PTHR10302; PTHR10302; 1.
DR Pfam; PF00436; SSB; 1.
DR SUPFAM; SSF50249; SSF50249; 1.
DR TIGRFAMs; TIGR00621; ssb; 1.
DR PROSITE; PS50935; SSB; 1.
PE 1: Evidence at protein level;
KW 3D-structure; DNA-binding; Reference proteome.
FT CHAIN 1..164
FT /note="Single-stranded DNA-binding protein"
FT /id="PRO_0000096069"
FT DOMAIN 1..110
FT /note="SSB"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00984"
FT REGION 120..164
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 121..145
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT STRAND 6..15
FT /evidence="ECO:0007829|PDB:1UE1"
FT STRAND 18..21
FT /evidence="ECO:0007829|PDB:1UE1"
FT TURN 23..25
FT /evidence="ECO:0007829|PDB:1UE7"
FT STRAND 27..35
FT /evidence="ECO:0007829|PDB:1UE1"
FT STRAND 42..45
FT /evidence="ECO:0007829|PDB:1UE1"
FT STRAND 53..60
FT /evidence="ECO:0007829|PDB:1UE1"
FT HELIX 61..70
FT /evidence="ECO:0007829|PDB:1UE1"
FT STRAND 76..88
FT /evidence="ECO:0007829|PDB:1UE1"
FT STRAND 90..94
FT /evidence="ECO:0007829|PDB:1UE1"
FT STRAND 96..108
FT /evidence="ECO:0007829|PDB:1UE1"
FT STRAND 111..116
FT /evidence="ECO:0007829|PDB:1UE1"
SQ SEQUENCE 164 AA; 17353 MW; 14797F8379FC2036 CRC64;
MAGDTTITIV GNLTADPELR FTPSGAAVAN FTVASTPRIY DRQTGEWKDG EALFLRCNIW
REAAENVAES LTRGARVIVS GRLKQRSFET REGEKRTVIE VEVDEIGPSL RYATAKVNKA
SRSGGFGSGS RPAPAQTSSA SGDDPWGSAP ASGSFGGGDD EPPF
