SSB_THET8
ID SSB_THET8 Reviewed; 263 AA.
AC Q5SLP9; Q70M05;
DT 29-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 25-MAY-2022, entry version 98.
DE RecName: Full=Single-stranded DNA-binding protein {ECO:0000255|HAMAP-Rule:MF_00984};
DE Short=SSB {ECO:0000255|HAMAP-Rule:MF_00984};
GN Name=ssb; OrderedLocusNames=TTHA0244;
OS Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
OC Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.
OX NCBI_TaxID=300852;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SUBUNIT.
RX PubMed=12368464; DOI=10.1099/00221287-148-10-3307;
RA Dabrowski S., Olszewski M., Piatek R., Brillowska-Dabrowska A., Konopa G.,
RA Kur J.;
RT "Identification and characterization of single-stranded-DNA-binding
RT proteins from Thermus thermophilus and Thermus aquaticus -- new arrangement
RT of binding domains.";
RL Microbiology 148:3307-3315(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Perales C., Cava F., Berenguer J.;
RT "One step beyond PCR and RT-PCR: the SSB protein from Thermus
RT thermophilus.";
RL Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 27634 / DSM 579 / HB8;
RA Masui R., Kurokawa K., Nakagawa N., Tokunaga F., Koyama Y., Shibata T.,
RA Oshima T., Yokoyama S., Yasunaga T., Kuramitsu S.;
RT "Complete genome sequence of Thermus thermophilus HB8.";
RL Submitted (NOV-2004) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: Plays an important role in DNA replication, recombination and
CC repair. Binds to ssDNA and to an array of partner proteins to recruit
CC them to their sites of action during DNA metabolism.
CC {ECO:0000255|HAMAP-Rule:MF_00984}.
CC -!- SUBUNIT: Homodimer. {ECO:0000269|PubMed:12368464}.
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DR EMBL; AF079160; AAC28386.2; -; Genomic_DNA.
DR EMBL; AJ564626; CAD92097.1; -; Genomic_DNA.
DR EMBL; AP008226; BAD70067.1; -; Genomic_DNA.
DR RefSeq; WP_011227803.1; NC_006461.1.
DR RefSeq; YP_143510.1; NC_006461.1.
DR PDB; 2CWA; X-ray; 1.96 A; A=1-263.
DR PDBsum; 2CWA; -.
DR AlphaFoldDB; Q5SLP9; -.
DR SMR; Q5SLP9; -.
DR STRING; 300852.55771626; -.
DR EnsemblBacteria; BAD70067; BAD70067; BAD70067.
DR GeneID; 3167973; -.
DR KEGG; ttj:TTHA0244; -.
DR PATRIC; fig|300852.9.peg.244; -.
DR eggNOG; COG0629; Bacteria.
DR HOGENOM; CLU_087573_0_0_0; -.
DR OMA; ARGMNHV; -.
DR EvolutionaryTrace; Q5SLP9; -.
DR Proteomes; UP000000532; Chromosome.
DR GO; GO:0003697; F:single-stranded DNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006310; P:DNA recombination; IEA:UniProtKB-UniRule.
DR GO; GO:0006281; P:DNA repair; IEA:UniProtKB-UniRule.
DR GO; GO:0006260; P:DNA replication; IEA:UniProtKB-UniRule.
DR CDD; cd04496; SSB_OBF; 2.
DR Gene3D; 2.40.50.140; -; 2.
DR HAMAP; MF_00984; SSB; 1.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR000424; Primosome_PriB/ssb.
DR InterPro; IPR011344; ssDNA-bd.
DR PANTHER; PTHR10302; PTHR10302; 2.
DR Pfam; PF00436; SSB; 2.
DR SUPFAM; SSF50249; SSF50249; 2.
DR TIGRFAMs; TIGR00621; ssb; 2.
DR PROSITE; PS50935; SSB; 2.
PE 1: Evidence at protein level;
KW 3D-structure; DNA damage; DNA recombination; DNA repair; DNA replication;
KW DNA-binding; Reference proteome; Repeat.
FT CHAIN 1..263
FT /note="Single-stranded DNA-binding protein"
FT /id="PRO_0000096150"
FT DOMAIN 5..108
FT /note="SSB 1"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00984"
FT DOMAIN 128..227
FT /note="SSB 2"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00984"
FT REGION 224..263
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 258..263
FT /note="Important for interaction with partner proteins"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00984"
FT CONFLICT 93
FT /note="R -> K (in Ref. 2; CAD92097)"
FT /evidence="ECO:0000305"
FT CONFLICT 241
FT /note="V -> A (in Ref. 2; CAD92097)"
FT /evidence="ECO:0000305"
FT CONFLICT 260
FT /note="E -> D (in Ref. 1 and 2)"
FT /evidence="ECO:0000305"
FT STRAND 5..16
FT /evidence="ECO:0007829|PDB:2CWA"
FT STRAND 19..22
FT /evidence="ECO:0007829|PDB:2CWA"
FT STRAND 28..39
FT /evidence="ECO:0007829|PDB:2CWA"
FT STRAND 49..59
FT /evidence="ECO:0007829|PDB:2CWA"
FT HELIX 60..64
FT /evidence="ECO:0007829|PDB:2CWA"
FT TURN 65..69
FT /evidence="ECO:0007829|PDB:2CWA"
FT STRAND 75..85
FT /evidence="ECO:0007829|PDB:2CWA"
FT STRAND 96..107
FT /evidence="ECO:0007829|PDB:2CWA"
FT STRAND 114..116
FT /evidence="ECO:0007829|PDB:2CWA"
FT STRAND 122..125
FT /evidence="ECO:0007829|PDB:2CWA"
FT STRAND 128..139
FT /evidence="ECO:0007829|PDB:2CWA"
FT STRAND 142..145
FT /evidence="ECO:0007829|PDB:2CWA"
FT STRAND 151..161
FT /evidence="ECO:0007829|PDB:2CWA"
FT STRAND 169..178
FT /evidence="ECO:0007829|PDB:2CWA"
FT HELIX 179..185
FT /evidence="ECO:0007829|PDB:2CWA"
FT STRAND 193..206
FT /evidence="ECO:0007829|PDB:2CWA"
FT STRAND 208..210
FT /evidence="ECO:0007829|PDB:2CWA"
FT STRAND 212..224
FT /evidence="ECO:0007829|PDB:2CWA"
SQ SEQUENCE 263 AA; 29882 MW; 7BD2BC31AE3B9214 CRC64;
MARGLNRVFL IGALATRPDM RYTPAGLAIL DLTLAGQDLL LSDNGGEREV SWYHRVRLLG
RQAEMWGDLL DQGQLVFVEG RLEYRQWERE GERRSELQIR ADFLDPLDDR GKERAEDSRG
QPRLRAALNQ VFLMGNLTRD PELRYTPQGT AVARLGLAVN ERRQGAEERT HFVEVQAWRD
LAEWAAELRK GDGLFVIGRL VNDSWTSSSG ERRFQTRVEA LRLERPTRGP AQAGGSRSRE
VQTGGVDIDE GLEDFPPEEE LPF
