SSC14_ARATH
ID SSC14_ARATH Reviewed; 1031 AA.
AC Q8W1Y0; Q940G1; Q9LF01;
DT 01-OCT-2014, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Sister chromatid cohesion 1 protein 4;
DE Short=AtRAD21-3;
DE Short=AtRAD21.3;
GN Name=SYN4; OrderedLocusNames=At5g16270; ORFNames=T21H19.190;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DISRUPTION PHENOTYPE, AND TISSUE
RP SPECIFICITY.
RC STRAIN=cv. Columbia;
RX PubMed=16488915; DOI=10.1093/jxb/erj083;
RA da Costa-Nunes J.A., Bhatt A.M., O'Shea S., West C.E., Bray C.M.,
RA Grossniklaus U., Dickinson H.G.;
RT "Characterization of the three Arabidopsis thaliana RAD21 cohesins reveals
RT differential responses to ionizing radiation.";
RL J. Exp. Bot. 57:971-983(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130714; DOI=10.1038/35048507;
RA Tabata S., Kaneko T., Nakamura Y., Kotani H., Kato T., Asamizu E.,
RA Miyajima N., Sasamoto S., Kimura T., Hosouchi T., Kawashima K., Kohara M.,
RA Matsumoto M., Matsuno A., Muraki A., Nakayama S., Nakazaki N., Naruo K.,
RA Okumura S., Shinpo S., Takeuchi C., Wada T., Watanabe A., Yamada M.,
RA Yasuda M., Sato S., de la Bastide M., Huang E., Spiegel L., Gnoj L.,
RA O'Shaughnessy A., Preston R., Habermann K., Murray J., Johnson D.,
RA Rohlfing T., Nelson J., Stoneking T., Pepin K., Spieth J., Sekhon M.,
RA Armstrong J., Becker M., Belter E., Cordum H., Cordes M., Courtney L.,
RA Courtney W., Dante M., Du H., Edwards J., Fryman J., Haakensen B.,
RA Lamar E., Latreille P., Leonard S., Meyer R., Mulvaney E., Ozersky P.,
RA Riley A., Strowmatt C., Wagner-McPherson C., Wollam A., Yoakum M., Bell M.,
RA Dedhia N., Parnell L., Shah R., Rodriguez M., Hoon See L., Vil D.,
RA Baker J., Kirchoff K., Toth K., King L., Bahret A., Miller B., Marra M.A.,
RA Martienssen R., McCombie W.R., Wilson R.K., Murphy G., Bancroft I.,
RA Volckaert G., Wambutt R., Duesterhoeft A., Stiekema W., Pohl T.,
RA Entian K.-D., Terryn N., Hartley N., Bent E., Johnson S., Langham S.-A.,
RA McCullagh B., Robben J., Grymonprez B., Zimmermann W., Ramsperger U.,
RA Wedler H., Balke K., Wedler E., Peters S., van Staveren M., Dirkse W.,
RA Mooijman P., Klein Lankhorst R., Weitzenegger T., Bothe G., Rose M.,
RA Hauf J., Berneiser S., Hempel S., Feldpausch M., Lamberth S.,
RA Villarroel R., Gielen J., Ardiles W., Bents O., Lemcke K., Kolesov G.,
RA Mayer K.F.X., Rudd S., Schoof H., Schueller C., Zaccaria P., Mewes H.-W.,
RA Bevan M., Fransz P.F.;
RT "Sequence and analysis of chromosome 5 of the plant Arabidopsis thaliana.";
RL Nature 408:823-826(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 672-1031.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RC STRAIN=cv. Columbia;
RX PubMed=19533160; DOI=10.1007/s00412-009-0220-x;
RA Schubert V., Weissleder A., Ali H., Fuchs J., Lermontova I., Meister A.,
RA Schubert I.;
RT "Cohesin gene defects may impair sister chromatid alignment and genome
RT stability in Arabidopsis thaliana.";
RL Chromosoma 118:591-605(2009).
CC -!- FUNCTION: Involved in sister chromatid and centromere cohesion during
CC mitosis. {ECO:0000269|PubMed:16488915, ECO:0000269|PubMed:19533160}.
CC -!- SUBUNIT: Component of the cohesin complex. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250}. Chromosome, centromere
CC {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in tissues containing dividing cells such
CC as seedlings, flower buds, flowers and inflorescence meristem tissue.
CC {ECO:0000269|PubMed:16488915}.
CC -!- DISRUPTION PHENOTYPE: Decreased sister chromatid alignment along arms
CC in tetraploid cells (4C) nuclei and impaired sister centromere
CC cohesion, associated with a high frequency of anaphases with bridges.
CC Slightly slower development leading to delayed flowering.
CC {ECO:0000269|PubMed:16488915, ECO:0000269|PubMed:19533160}.
CC -!- SIMILARITY: Belongs to the rad21 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK96855.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=CAC01868.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR EMBL; AF400129; AAL62060.1; -; mRNA.
DR EMBL; AL391148; CAC01868.1; ALT_SEQ; Genomic_DNA.
DR EMBL; CP002688; AED92267.1; -; Genomic_DNA.
DR EMBL; AY054664; AAK96855.1; ALT_INIT; mRNA.
DR EMBL; AY072469; AAL66884.1; -; mRNA.
DR PIR; T51497; T51497.
DR RefSeq; NP_197131.2; NM_121632.4.
DR AlphaFoldDB; Q8W1Y0; -.
DR SMR; Q8W1Y0; -.
DR BioGRID; 16763; 9.
DR STRING; 3702.AT5G16270.1; -.
DR iPTMnet; Q8W1Y0; -.
DR PaxDb; Q8W1Y0; -.
DR PRIDE; Q8W1Y0; -.
DR ProteomicsDB; 234101; -.
DR EnsemblPlants; AT5G16270.1; AT5G16270.1; AT5G16270.
DR GeneID; 831487; -.
DR Gramene; AT5G16270.1; AT5G16270.1; AT5G16270.
DR KEGG; ath:AT5G16270; -.
DR Araport; AT5G16270; -.
DR TAIR; locus:2181457; AT5G16270.
DR eggNOG; KOG1213; Eukaryota.
DR HOGENOM; CLU_007674_0_0_1; -.
DR InParanoid; Q8W1Y0; -.
DR OMA; HNEPMAV; -.
DR OrthoDB; 171779at2759; -.
DR PhylomeDB; Q8W1Y0; -.
DR PRO; PR:Q8W1Y0; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q8W1Y0; baseline and differential.
DR Genevisible; Q8W1Y0; AT.
DR GO; GO:0000775; C:chromosome, centromeric region; IEA:UniProtKB-SubCell.
DR GO; GO:0008278; C:cohesin complex; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR GO; GO:0070601; P:centromeric sister chromatid cohesion; IMP:UniProtKB.
DR GO; GO:0006302; P:double-strand break repair; IMP:TAIR.
DR GO; GO:1990414; P:replication-born double-strand break repair via sister chromatid exchange; IBA:GO_Central.
DR GO; GO:0007062; P:sister chromatid cohesion; IMP:TAIR.
DR Gene3D; 1.10.10.580; -; 1.
DR InterPro; IPR039781; Rad21/Rec8-like.
DR InterPro; IPR006909; Rad21/Rec8_C_eu.
DR InterPro; IPR006910; Rad21_Rec8_N.
DR InterPro; IPR023093; ScpA-like_C.
DR InterPro; IPR036390; WH_DNA-bd_sf.
DR PANTHER; PTHR12585; PTHR12585; 1.
DR Pfam; PF04824; Rad21_Rec8; 1.
DR Pfam; PF04825; Rad21_Rec8_N; 1.
DR SUPFAM; SSF46785; SSF46785; 1.
PE 2: Evidence at transcript level;
KW Cell cycle; Cell division; Centromere; Chromosome; Chromosome partition;
KW Mitosis; Nucleus; Reference proteome.
FT CHAIN 1..1031
FT /note="Sister chromatid cohesion 1 protein 4"
FT /id="PRO_0000430279"
FT REGION 461..481
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 661..703
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 742..772
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 803..835
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 879..908
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 545..552
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000250"
FT COMPBIAS 661..675
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 742..767
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 803..823
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CONFLICT 1030
FT /note="I -> V (in Ref. 4; AAK96855/AAL66884)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 1031 AA; 114059 MW; 029B2A9229649AD1 CRC64;
MFYSQFILAK KGPLGTIWIA AHLERKLRKN QVADTDIGVS VDSILFPEAP IALRLSSHLL
LGVVRIYSRK VNYLFDDCSE ALLKVKQAFR SAAVDLPPEE STAPYHSITL PETFDLDDFE
LPDNEIFQGN YVDHHVSTKE QITLQDTMDG VVYSTSQFGL DERFGDGDTS QAALDLDEAV
FQDKDVIGSD DEGVPGIDHN AYLDAAAPGI KDSMEGVSEA MPMDFNEEQV EDLAMNNEFI
EDAQAPQTPG LVEVPNSSSV REQMACDDHM DVEDLNAEEG IKSSGELNAN EMPKRGEDLS
SEYNAPESAV TPVEVDKSQI DENVNTQNEP EEERAEHVHV TSPCCSHITT EMEDPGQVMN
EAGANVVPDK PDAVPPLETP GEENRDHFAI ATEVNQETDS SLQGDEQAYS RPDGQLNNAH
ETDEQLGNLT GFTDSDFPPP EKVLAVPNRQ GDGNDFMVES TPDKEDPGTC NDDAGNNNIT
GKKRTFTEST LTAESLNSVE SVGLIQSKRT ADSVPDDDDL LSSILVGKSS FLKMRPTPVL
EPATTKRLRS APRSTATKRK VLMDDPMVLH GDIIRQQLTN TEDIRRVRKK APCTVPEIVM
LQRQALEDGL FKEPIFTGMS VELVSLHTEP YDLRGIMIIE NDDRHASVGA VEDNECSVTA
VEENKTEESS DPQAHPNDCE EQPGTAHTHP QEEQTINQQE ELKDDNELAE KSDLEVLKEG
NGAADEVNLV VIDDVSQIPS EEKLDRVEDL QVEESHENHD GEGGQDVCAD PNEKSCTDVI
EIAEGDTDIN PIFNEMDLKV EDELPHEDEK TDASAEVSEL GRDDQTPCDN TVGSTETGCL
EAGDLSNMAL ENCNEPLVEA NSDGLNPETE SYNKYEPHNE MSNEEASMQN ALDGEHTSRD
GLMGDNDEMD TMENAHDTGF LNVDDDEVDE DHEEDDIQYD DETRLLENSG WSSRTRAVAK
YLQTLFDKET ENGKNVLVAD KLLAGKTRKE ASRMFFETLV LKTRDYIQVE QGKPYESIII
KPRPKLTKSI F
