SSED_SALTY
ID SSED_SALTY Reviewed; 195 AA.
AC Q9R803; O84948; Q7CQL8;
DT 02-MAR-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 88.
DE RecName: Full=Secreted effector protein SseD;
DE AltName: Full=Secretion system effector D;
GN Name=sseD; OrderedLocusNames=STM1401;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 14028 / SGSC 2980 / CDC 6516-60 / NCTC 12023;
RX PubMed=9786193; DOI=10.1046/j.1365-2958.1998.01047.x;
RA Hensel M., Shea J.E., Waterman S.R., Mundy R., Nikolaus T., Banks G.,
RA Vazquez-Torres A., Gleeson C., Fang F.C., Holden D.W.;
RT "Genes encoding putative effector proteins of the type III secretion system
RT of Salmonella pathogenicity island 2 are required for bacterial virulence
RT and proliferation in macrophages.";
RL Mol. Microbiol. 30:163-174(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=SL1344;
RX PubMed=9786194; DOI=10.1046/j.1365-2958.1998.01048.x;
RA Cirillo D.M., Valdivia R.H., Monack D.M., Falkow S.;
RT "Macrophage-dependent induction of the Salmonella pathogenicity island 2
RT type III secretion system and its role in intracellular survival.";
RL Mol. Microbiol. 30:175-188(1998).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [4]
RP PROTEIN SEQUENCE OF 2-9, FUNCTION, SUBUNIT, SUBCELLULAR LOCATION, AND
RP SECRETION VIA TYPE III SECRETION SYSTEM.
RC STRAIN=ATCC 14028 / SGSC 2980 / CDC 6516-60 / NCTC 12023;
RX PubMed=11567004; DOI=10.1128/jb.183.20.6036-6045.2001;
RA Nikolaus T., Deiwick J., Rappl C., Freeman J.A., Schroder W., Miller S.I.,
RA Hensel M.;
RT "SseBCD proteins are secreted by the type III secretion system of
RT Salmonella pathogenicity island 2 and function as a translocon.";
RL J. Bacteriol. 183:6036-6045(2001).
RN [5]
RP FUNCTION IN VIRULENCE, SUBCELLULAR LOCATION, AND SECRETION VIA TYPE III
RP SECRETION SYSTEM.
RC STRAIN=SL1344;
RX PubMed=11159962; DOI=10.1128/iai.69.2.737-743.2001;
RA Klein J.R., Jones B.D.;
RT "Salmonella pathogenicity island 2-encoded proteins SseC and SseD are
RT essential for virulence and are substrates of the type III secretion
RT system.";
RL Infect. Immun. 69:737-743(2001).
RN [6]
RP INTERACTION WITH SSEA.
RC STRAIN=ATCC 14028 / SGSC 2980 / CDC 6516-60 / NCTC 12023;
RX PubMed=12724372; DOI=10.1099/mic.0.26190-0;
RA Ruiz-Albert J., Mundy R., Yu X.J., Beuzon C.R., Holden D.W.;
RT "SseA is a chaperone for the SseB and SseD translocon components of the
RT Salmonella pathogenicity-island-2-encoded type III secretion system.";
RL Microbiology 149:1103-1111(2003).
RN [7]
RP INTERACTION WITH SSEA.
RC STRAIN=SL1344;
RX PubMed=15256549; DOI=10.1099/mic.0.26997-0;
RA Zurawski D.V., Stein M.A.;
RT "The SPI2-encoded SseA chaperone has discrete domains required for SseB
RT stabilization and export, and binds within the C-terminus of SseB and
RT SseD.";
RL Microbiology 150:2055-2068(2004).
RN [8]
RP ROLE IN SPVB EXPORT, AND DISRUPTION PHENOTYPE.
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=18248436; DOI=10.1111/j.1574-695x.2007.00364.x;
RA Browne S.H., Hasegawa P., Okamoto S., Fierer J., Guiney D.G.;
RT "Identification of Salmonella SPI-2 secretion system components required
RT for SpvB-mediated cytotoxicity in macrophages and virulence in mice.";
RL FEMS Immunol. Med. Microbiol. 52:194-201(2008).
CC -!- FUNCTION: Effector proteins function to alter host cell physiology and
CC promote bacterial survival in host tissues. May act as a translocator
CC that mediates translocation of SPI-2 TTSS effector proteins from
CC intraphagosomal bacterial cells into the host cells.
CC {ECO:0000269|PubMed:11159962, ECO:0000269|PubMed:11567004,
CC ECO:0000269|PubMed:18248436}.
CC -!- SUBUNIT: May form a complex with SseB and SseC. SseB is required for
CC correct localization of SseD on the bacterial cell surface. Binds to
CC the chaperone SseA. {ECO:0000269|PubMed:11567004}.
CC -!- INTERACTION:
CC Q9R803; O84944: sseA; NbExp=2; IntAct=EBI-2272067, EBI-2030631;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:11159962,
CC ECO:0000269|PubMed:11567004}. Cell surface
CC {ECO:0000269|PubMed:11159962, ECO:0000269|PubMed:11567004}.
CC Note=Secreted via type III secretion system 2 (SPI-2 TTSS). After
CC secretion, localizes mainly on the surface of the bacterial cell.
CC {ECO:0000269|PubMed:11159962, ECO:0000269|PubMed:11567004}.
CC -!- DISRUPTION PHENOTYPE: Disruption prevents SpvB-induced F-actin
CC depolymerization in human macrophages without affecting intra-bacterial
CC SpvB protein levels. {ECO:0000269|PubMed:18248436}.
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DR EMBL; AJ224892; CAA12188.1; -; Genomic_DNA.
DR EMBL; AF020808; AAC28882.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL20325.1; -; Genomic_DNA.
DR RefSeq; NP_460366.1; NC_003197.2.
DR RefSeq; WP_000388323.1; NC_003197.2.
DR AlphaFoldDB; Q9R803; -.
DR IntAct; Q9R803; 1.
DR STRING; 99287.STM1401; -.
DR TCDB; 1.C.36.5.1; the bacterial type iii-target cell pore (iiitcp) family.
DR PaxDb; Q9R803; -.
DR EnsemblBacteria; AAL20325; AAL20325; STM1401.
DR GeneID; 1252919; -.
DR KEGG; stm:STM1401; -.
DR PATRIC; fig|99287.12.peg.1485; -.
DR HOGENOM; CLU_120485_0_0_6; -.
DR OMA; IWTKLME; -.
DR BioCyc; SENT99287:STM1401-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0009986; C:cell surface; IDA:UniProtKB.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030254; P:protein secretion by the type III secretion system; IDA:UniProtKB.
DR InterPro; IPR008611; EaeB.
DR Pfam; PF05802; EspB; 2.
PE 1: Evidence at protein level;
KW Coiled coil; Direct protein sequencing; Protein transport;
KW Reference proteome; Secreted; Transport; Virulence.
FT CHAIN 1..195
FT /note="Secreted effector protein SseD"
FT /id="PRO_0000391717"
FT COILED 44..80
FT /evidence="ECO:0000255"
FT CONFLICT 153
FT /note="G -> R (in Ref. 2; AAC28882)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 195 AA; 20592 MW; 45F95BBEB726D837 CRC64;
MEASNVALVL PAPSLLTPSS TPSPSGEGMG TESMLLLFDD IWMKLMELAK KLRDIMRSYN
VEKQRLAWEL QVNVLQTQMK TIDEAFRASM ITAGGAMLSG VLTIGLGAVG GETGLIAGQA
VGHTAGGVMG LGAGVAQRQS DQDKAIADLQ QNGAQSYNKS LTEIMEKATE IMQQIIGVGS
SLVTVLAEIL RALTR
