SSEI_SALTY
ID SSEI_SALTY Reviewed; 322 AA.
AC Q8ZQ79; Q9KIC1; Q9KIK1;
DT 09-FEB-2010, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2002, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Secreted effector protein SseI;
GN Name=sseI; Synonyms=srfH; OrderedLocusNames=STM1051;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 14028s / SGSG 2262;
RX PubMed=10844662; DOI=10.1046/j.1365-2958.2000.01902.x;
RA Worley M.J., Ching K.H., Heffron F.;
RT "Salmonella SsrB activates a global regulon of horizontally acquired
RT genes.";
RL Mol. Microbiol. 36:749-761(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], SUBCELLULAR LOCATION, AND SECRETION VIA
RP TYPE III SECRETION SYSTEM.
RC STRAIN=ATCC 14028s / SGSG 2262;
RX PubMed=10861017; DOI=10.1073/pnas.97.13.7539;
RA Miao E.A., Miller S.I.;
RT "A conserved amino acid sequence directing intracellular type III secretion
RT by Salmonella typhimurium.";
RL Proc. Natl. Acad. Sci. U.S.A. 97:7539-7544(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [4]
RP FUNCTION, AND INTERACTION WITH HOST TRIP6.
RC STRAIN=ATCC 14028s / SGSG 2262;
RX PubMed=17095609; DOI=10.1073/pnas.0604054103;
RA Worley M.J., Nieman G.S., Geddes K., Heffron F.;
RT "Salmonella typhimurium disseminates within its host by manipulating the
RT motility of infected cells.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:17915-17920(2006).
RN [5]
RP FUNCTION, INTERACTION WITH HOST IQGAP1, SUBCELLULAR LOCATION, AND
RP MUTAGENESIS OF CYS-178; HIS-216 AND ASP-231.
RC STRAIN=SL1344;
RX PubMed=19956712; DOI=10.1371/journal.ppat.1000671;
RA McLaughlin L.M., Govoni G.R., Gerke C., Gopinath S., Peng K., Laidlaw G.,
RA Chien Y.H., Jeong H.W., Li Z., Brown M.D., Sacks D.B., Monack D.;
RT "The Salmonella SPI2 effector SseI mediates long-term systemic infection by
RT modulating host cell migration.";
RL PLoS Pathog. 5:E1000671-E1000671(2009).
CC -!- FUNCTION: Effector proteins function to alter host cell physiology and
CC promote bacterial survival in host tissues. This protein is required to
CC maintain a long-term chronic systemic infection in mice. It inhibits
CC normal cell migration of primary macrophages and dendritic cells, by a
CC mechanism that involves interaction with the host factor IQGAP1, an
CC important regulator of the cytoskeleton and cell migration. Also
CC accelerates the systemic spread of infection from the gastrointestinal
CC tract to the bloodstream, probably by interacting with host TRIP6.
CC {ECO:0000269|PubMed:17095609, ECO:0000269|PubMed:19956712}.
CC -!- SUBUNIT: Interacts with host IQGAP1 and host TRIP6 (thyroid receptor-
CC interacting protein 6). {ECO:0000269|PubMed:17095609,
CC ECO:0000269|PubMed:19956712}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10861017}. Host
CC cytoplasm {ECO:0000269|PubMed:10861017, ECO:0000269|PubMed:19956712}.
CC Note=Secreted via type III secretion system 2 (SPI-2 TTSS), and
CC delivered into the host cytoplasm. Colocalizes with IQGAP1 and actin at
CC the cell periphery. {ECO:0000269|PubMed:10861017}.
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DR EMBL; AF231470; AAF73990.2; -; Genomic_DNA.
DR EMBL; AF236075; AAF82082.1; -; Genomic_DNA.
DR EMBL; AE006468; AAL19985.1; -; Genomic_DNA.
DR RefSeq; WP_001533476.1; NC_003197.2.
DR PDB; 4G29; X-ray; 1.70 A; A=137-322.
DR PDB; 4G2B; X-ray; 2.05 A; A/B=137-322.
DR PDBsum; 4G29; -.
DR PDBsum; 4G2B; -.
DR AlphaFoldDB; Q8ZQ79; -.
DR SMR; Q8ZQ79; -.
DR DIP; DIP-61315N; -.
DR IntAct; Q8ZQ79; 1.
DR STRING; 90371.CY43_05385; -.
DR PaxDb; Q8ZQ79; -.
DR EnsemblBacteria; AAL19985; AAL19985; STM1051.
DR PATRIC; fig|99287.12.peg.1115; -.
DR HOGENOM; CLU_077951_0_0_6; -.
DR OMA; SADEWAC; -.
DR BioCyc; SENT99287:STM1051-MON; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR InterPro; IPR028907; Tox-PLDMTX_dom.
DR Pfam; PF15645; Tox-PLDMTX; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Host cytoplasm; Reference proteome; Secreted; Virulence.
FT CHAIN 1..322
FT /note="Secreted effector protein SseI"
FT /id="PRO_0000391491"
FT MUTAGEN 178
FT /note="C->A: Loss of activity. No change in IQGAP1
FT binding."
FT /evidence="ECO:0000269|PubMed:19956712"
FT MUTAGEN 216
FT /note="H->A: Does not affect regulation of macrophage
FT migration."
FT /evidence="ECO:0000269|PubMed:19956712"
FT MUTAGEN 231
FT /note="D->A: Does not affect regulation of macrophage
FT migration."
FT /evidence="ECO:0000269|PubMed:19956712"
FT CONFLICT 103
FT /note="D -> G (in Ref. 1; AAF73990 and 2; AAF82082)"
FT /evidence="ECO:0000305"
FT TURN 150..153
FT /evidence="ECO:0007829|PDB:4G29"
FT HELIX 154..162
FT /evidence="ECO:0007829|PDB:4G29"
FT HELIX 165..172
FT /evidence="ECO:0007829|PDB:4G29"
FT HELIX 178..191
FT /evidence="ECO:0007829|PDB:4G29"
FT STRAND 195..207
FT /evidence="ECO:0007829|PDB:4G29"
FT STRAND 214..223
FT /evidence="ECO:0007829|PDB:4G29"
FT STRAND 226..231
FT /evidence="ECO:0007829|PDB:4G29"
FT HELIX 234..237
FT /evidence="ECO:0007829|PDB:4G29"
FT TURN 238..241
FT /evidence="ECO:0007829|PDB:4G29"
FT STRAND 249..252
FT /evidence="ECO:0007829|PDB:4G29"
FT HELIX 253..262
FT /evidence="ECO:0007829|PDB:4G29"
FT STRAND 268..276
FT /evidence="ECO:0007829|PDB:4G29"
FT HELIX 277..283
FT /evidence="ECO:0007829|PDB:4G29"
FT HELIX 291..293
FT /evidence="ECO:0007829|PDB:4G29"
FT STRAND 300..302
FT /evidence="ECO:0007829|PDB:4G29"
FT HELIX 306..312
FT /evidence="ECO:0007829|PDB:4G29"
SQ SEQUENCE 322 AA; 36831 MW; 1A896A8995660094 CRC64;
MPFHIGSGCL PAIISNRRIY RIAWSDTPPE MSSWEKMKEF FCSTHQAEAL ECIWTICHPP
AGTTREDVVS RFELLRTLAY DGWEENIHSG LHGENYFCIL DEDSQEILSV TLDDVGNYTV
NCQGYSETHH LTMATEPGVE RTDITYNLTS DIDAAAYLEE LKQNPIINNK IMNPVGQCES
LMTPVSNFMN EKGFDNIRYR GIFIWDKPTE EIPTNHFAVV GNKEGKDYVF DVSAHQFENR
GMSNLNGPLI LSADEWVCKY RMATRRKLIY YTDFSNSSIA ANAYDALPRE LESESMAGKV
FVTSPRWFNT FKKQKYSLIG KM
