SSF2_YEAST
ID SSF2_YEAST Reviewed; 453 AA.
AC Q12153; D6VSU1;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Ribosome biogenesis protein SSF2;
GN Name=SSF2; OrderedLocusNames=YDR312W; ORFNames=D9740.2;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=7748491; DOI=10.1089/dna.1995.14.411;
RA Yu Y., Hirsch J.P.;
RT "An essential gene pair in Saccharomyces cerevisiae with a potential role
RT in mating.";
RL DNA Cell Biol. 14:411-418(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169867;
RA Jacq C., Alt-Moerbe J., Andre B., Arnold W., Bahr A., Ballesta J.P.G.,
RA Bargues M., Baron L., Becker A., Biteau N., Bloecker H., Blugeon C.,
RA Boskovic J., Brandt P., Brueckner M., Buitrago M.J., Coster F.,
RA Delaveau T., del Rey F., Dujon B., Eide L.G., Garcia-Cantalejo J.M.,
RA Goffeau A., Gomez-Peris A., Granotier C., Hanemann V., Hankeln T.,
RA Hoheisel J.D., Jaeger W., Jimenez A., Jonniaux J.-L., Kraemer C.,
RA Kuester H., Laamanen P., Legros Y., Louis E.J., Moeller-Rieker S.,
RA Monnet A., Moro M., Mueller-Auer S., Nussbaumer B., Paricio N., Paulin L.,
RA Perea J., Perez-Alonso M., Perez-Ortin J.E., Pohl T.M., Prydz H.,
RA Purnelle B., Rasmussen S.W., Remacha M.A., Revuelta J.L., Rieger M.,
RA Salom D., Saluz H.P., Saiz J.E., Saren A.-M., Schaefer M., Scharfe M.,
RA Schmidt E.R., Schneider C., Scholler P., Schwarz S., Soler-Mira A.,
RA Urrestarazu L.A., Verhasselt P., Vissers S., Voet M., Volckaert G.,
RA Wagner G., Wambutt R., Wedler E., Wedler H., Woelfl S., Harris D.E.,
RA Bowman S., Brown D., Churcher C.M., Connor R., Dedman K., Gentles S.,
RA Hamlin N., Hunt S., Jones L., McDonald S., Murphy L.D., Niblett D.,
RA Odell C., Oliver K., Rajandream M.A., Richards C., Shore L., Walsh S.V.,
RA Barrell B.G., Dietrich F.S., Mulligan J.T., Allen E., Araujo R., Aviles E.,
RA Berno A., Carpenter J., Chen E., Cherry J.M., Chung E., Duncan M.,
RA Hunicke-Smith S., Hyman R.W., Komp C., Lashkari D., Lew H., Lin D.,
RA Mosedale D., Nakahara K., Namath A., Oefner P., Oh C., Petel F.X.,
RA Roberts D., Schramm S., Schroeder M., Shogren T., Shroff N., Winant A.,
RA Yelton M.A., Botstein D., Davis R.W., Johnston M., Andrews S., Brinkman R.,
RA Cooper J., Ding H., Du Z., Favello A., Fulton L., Gattung S., Greco T.,
RA Hallsworth K., Hawkins J., Hillier L.W., Jier M., Johnson D., Johnston L.,
RA Kirsten J., Kucaba T., Langston Y., Latreille P., Le T., Mardis E.,
RA Menezes S., Miller N., Nhan M., Pauley A., Peluso D., Rifkin L., Riles L.,
RA Taich A., Trevaskis E., Vignati D., Wilcox L., Wohldman P., Vaudin M.,
RA Wilson R., Waterston R., Albermann K., Hani J., Heumann K., Kleine K.,
RA Mewes H.-W., Zollner A., Zaccaria P.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome IV.";
RL Nature 387:75-78(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=9611192; DOI=10.1093/genetics/149.2.795;
RA Kim J., Hirsch J.P.;
RT "A nucleolar protein that affects mating efficiency in Saccharomyces
RT cerevisiae by altering the morphological response to pheromone.";
RL Genetics 149:795-805(1998).
RN [5]
RP FUNCTION.
RX PubMed=12702244; DOI=10.1111/j.1567-1364.2003.tb00136.x;
RA Bogengruber E., Briza P., Doppler E., Wimmer H., Koller L., Fasiolo F.,
RA Senger B., Hegemann J.H., Breitenbach M.;
RT "Functional analysis in yeast of the Brix protein superfamily involved in
RT the biogenesis of ribosomes.";
RL FEMS Yeast Res. 3:35-43(2003).
RN [6]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
CC -!- FUNCTION: Required for biogenesis of the 60S ribosomal subunit.
CC {ECO:0000269|PubMed:12702244}.
CC -!- SUBUNIT: Part of a complex that includes BRX1, RPF1, RPF2 and SSF1 or
CC SSF2.
CC -!- INTERACTION:
CC Q12153; Q06511: RRP15; NbExp=5; IntAct=EBI-18168, EBI-34602;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000269|PubMed:9611192}.
CC -!- MISCELLANEOUS: Present with 1350 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
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DR EMBL; U18114; AAA79981.1; -; Genomic_DNA.
DR EMBL; U28374; AAB64748.1; -; Genomic_DNA.
DR EMBL; BK006938; DAA12151.1; -; Genomic_DNA.
DR PIR; S50248; S50248.
DR RefSeq; NP_010598.3; NM_001180620.3.
DR AlphaFoldDB; Q12153; -.
DR SMR; Q12153; -.
DR BioGRID; 32365; 101.
DR DIP; DIP-6502N; -.
DR IntAct; Q12153; 15.
DR MINT; Q12153; -.
DR STRING; 4932.YDR312W; -.
DR iPTMnet; Q12153; -.
DR MaxQB; Q12153; -.
DR PaxDb; Q12153; -.
DR PRIDE; Q12153; -.
DR EnsemblFungi; YDR312W_mRNA; YDR312W; YDR312W.
DR GeneID; 851907; -.
DR KEGG; sce:YDR312W; -.
DR SGD; S000002720; SSF2.
DR VEuPathDB; FungiDB:YDR312W; -.
DR eggNOG; KOG2963; Eukaryota.
DR GeneTree; ENSGT00530000064158; -.
DR HOGENOM; CLU_026936_2_0_1; -.
DR InParanoid; Q12153; -.
DR OMA; CLVIHRG; -.
DR BioCyc; YEAST:G3O-29871-MON; -.
DR PRO; PR:Q12153; -.
DR Proteomes; UP000002311; Chromosome IV.
DR RNAct; Q12153; protein.
DR GO; GO:0005730; C:nucleolus; IDA:SGD.
DR GO; GO:0030687; C:preribosome, large subunit precursor; IBA:GO_Central.
DR GO; GO:0019843; F:rRNA binding; IDA:SGD.
DR GO; GO:0000027; P:ribosomal large subunit assembly; IMP:SGD.
DR GO; GO:0006364; P:rRNA processing; IEA:InterPro.
DR InterPro; IPR007109; Brix.
DR InterPro; IPR045112; PPAN-like.
DR PANTHER; PTHR12661; PTHR12661; 1.
DR Pfam; PF04427; Brix; 1.
DR SMART; SM00879; Brix; 1.
DR PROSITE; PS50833; BRIX; 1.
PE 1: Evidence at protein level;
KW Nucleus; Reference proteome; Ribosome biogenesis.
FT CHAIN 1..453
FT /note="Ribosome biogenesis protein SSF2"
FT /id="PRO_0000120260"
FT DOMAIN 26..348
FT /note="Brix"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00034"
FT REGION 1..22
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 275..327
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 373..453
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 275..289
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 373..394
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 406..420
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 438..453
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 453 AA; 51856 MW; 83306767C4F58816 CRC64;
MAKRRQKKRT HAQITPEQER DIPKSMVIRV GQTSLANHSL NQLVKDFRQI MQPHTAVKLK
ERKSNKLKDF VVMCGPLGVT HLFMFTQSEK TGNVSLKIAR TPQGPTVTFQ VLDYSLGRDI
KKFLKRPKSL NNDDVLNPPL LVLNGFSTSK RSDEDDQDVN VEKVIVSMFQ NIFPPLNPAR
TSLNSIKRIF MINKDRETGE ISMRHYFIDI REVEISRNLK RLYKAKNNLS KTVPNLHRKE
DISSLILDHD LGAYTSESEI EDDAIVRVVD NQDVKAKHSQ TSLSQKTPVK MTDNEEREKG
IEEEDVEMEE PKPSENSQPT PRKKAIKLTE LGPRLTLKLV KIEDGICSGK VLHHEFVQKS
SEEIKALEKR HAAKMRLKEQ RRKEQEENIA KKKAVKDAKK QRKLERRKAR AEEQGEGQGK
DGAMSDDGSS SSEDEHYSDV PEDLDSDLFS EVE
