SSG1_HORVU
ID SSG1_HORVU Reviewed; 603 AA.
AC P09842; Q8GS48; Q8L4L3; Q8LL06; Q8LL07; Q8LL08; Q8SA49;
DT 01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-JUL-1989, sequence version 1.
DT 03-AUG-2022, entry version 113.
DE RecName: Full=Granule-bound starch synthase 1, chloroplastic/amyloplastic;
DE EC=2.4.1.242;
DE AltName: Full=Granule-bound starch synthase I;
DE Short=GBSS-I;
DE Flags: Precursor;
GN Name=WAXY; Synonyms=259I16.5;
OS Hordeum vulgare (Barley).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Hordeinae; Hordeum.
OX NCBI_TaxID=4513;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=cv. Vogelsanger Gold; TISSUE=Leaf;
RX PubMed=2970062; DOI=10.1093/nar/16.14.7185;
RA Rhode W., Becker D., Salamini F.;
RT "Structural analysis of the waxy locus from Hordeum vulgare.";
RL Nucleic Acids Res. 16:7185-7186(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE, PROTEIN SEQUENCE OF 71-82, AND VARIANTS.
RC STRAIN=cv. CDC Alamo, cv. Iyatoma Mochi, cv. Oderbrucker, cv. SB 85750,
RC cv. Shikoku Hadaka 84, and cv. Waxy Oderbrucker;
RX PubMed=12226499; DOI=10.1104/pp.005454;
RA Patron N.J., Smith A.M., Fahy B.F., Hylton C.M., Naldrett M.J.,
RA Rossnagel B.G., Denyer K.;
RT "The altered pattern of amylose accumulation in the endosperm of low-
RT amylose barley cultivars is attributable to a single mutant allele of
RT granule-bound starch synthase I with a deletion in the 5'-non-coding
RT region.";
RL Plant Physiol. 130:190-198(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. Mochimugi-D, and cv. Shikoku Hadaka 84;
RX PubMed=12441646; DOI=10.1266/ggs.77.351;
RA Domon E., Saito A., Takeda K.;
RT "Comparison of the waxy locus sequence from a non-waxy strain and two waxy
RT mutants of spontaneous and artificial origins in barley.";
RL Genes Genet. Syst. 77:351-359(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=cv. Morex;
RA Ma J., SanMiguel P.J., Dubcovsky J., Shiloff B.A., Rostoks N., Jiang Z.,
RA Busso C.S., Kleinhofs A., Devos K.M., Ramakrishna W., Bennetzen J.L.;
RT "Comparative sequence analysis of Wx1 homologous regions in barley, maize,
RT pearl millet, rice, sorghum and diploid wheat.";
RL Submitted (JAN-2002) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP PROTEIN SEQUENCE OF 76-89.
RC STRAIN=cv. H354-295-2-5; TISSUE=Starchy endosperm;
RX PubMed=8125056; DOI=10.1002/elps.11501401169;
RA Flengsrud R.;
RT "Separation of acidic barley endosperm proteins by two-dimensional
RT electrophoresis.";
RL Electrophoresis 14:1060-1066(1993).
CC -!- FUNCTION: Required for the synthesis of amylose in endosperm.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[(1->4)-alpha-D-glucosyl](n) + an NDP-alpha-D-glucose =
CC [(1->4)-alpha-D-glucosyl](n+1) + a ribonucleoside 5'-diphosphate +
CC H(+); Xref=Rhea:RHEA:15873, Rhea:RHEA-COMP:9584, Rhea:RHEA-COMP:9587,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15444, ChEBI:CHEBI:57930,
CC ChEBI:CHEBI:76533; EC=2.4.1.242;
CC -!- PATHWAY: Glycan biosynthesis; starch biosynthesis.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast. Plastid, amyloplast.
CC Note=Amyloplast or chloroplast, granule-bound.
CC -!- POLYMORPHISM: Two groups of alleles can be distinguished based on the
CC presence of a 5 amino acid insertion at position 21.
CC -!- POLYMORPHISM: Position 287 is associated with waxy phenotype. Asp-287
CC is essential for starch synthase activity.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 1 family.
CC Bacterial/plant glycogen synthase subfamily. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; X07931; CAA30755.1; -; Genomic_DNA.
DR EMBL; X07932; CAA30756.1; -; mRNA.
DR EMBL; AF486514; AAM74048.1; -; mRNA.
DR EMBL; AF486519; AAM74052.1; -; mRNA.
DR EMBL; AF486515; AAM74049.1; -; mRNA.
DR EMBL; AF486517; AAM74050.1; -; mRNA.
DR EMBL; AF486518; AAM74051.1; -; mRNA.
DR EMBL; AF486520; AAM74053.1; -; mRNA.
DR EMBL; AB087716; BAC41202.1; -; Genomic_DNA.
DR EMBL; AB088761; BAC41203.1; -; Genomic_DNA.
DR EMBL; AF474373; AAL77109.1; -; Genomic_DNA.
DR PIR; S01727; YUBHY.
DR AlphaFoldDB; P09842; -.
DR SMR; P09842; -.
DR IntAct; P09842; 1.
DR CAZy; GT5; Glycosyltransferase Family 5.
DR EnsemblPlants; HORVU.MOREX.r2.7HG0535060.1.mrna1; HORVU.MOREX.r2.7HG0535060.1.mrna1; HORVU.MOREX.r2.7HG0535060.1.
DR Gramene; HORVU.MOREX.r2.7HG0535060.1.mrna1; HORVU.MOREX.r2.7HG0535060.1.mrna1; HORVU.MOREX.r2.7HG0535060.1.
DR BRENDA; 2.4.1.21; 2687.
DR UniPathway; UPA00152; -.
DR ExpressionAtlas; P09842; baseline and differential.
DR GO; GO:0009501; C:amyloplast; IEA:UniProtKB-SubCell.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0102502; F:ADP-glucose-starch glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0004373; F:glycogen (starch) synthase activity; IEA:InterPro.
DR GO; GO:0009011; F:starch synthase activity; IEA:EnsemblPlants.
DR GO; GO:0019252; P:starch biosynthetic process; IEA:UniProtKB-UniPathway.
DR HAMAP; MF_00484; Glycogen_synth; 1.
DR InterPro; IPR001296; Glyco_trans_1.
DR InterPro; IPR011835; GS/SS.
DR InterPro; IPR013534; Starch_synth_cat_dom.
DR Pfam; PF08323; Glyco_transf_5; 1.
DR Pfam; PF00534; Glycos_transf_1; 1.
DR TIGRFAMs; TIGR02095; glgA; 1.
PE 1: Evidence at protein level;
KW Amyloplast; Chloroplast; Direct protein sequencing; Glycosyltransferase;
KW Plastid; Starch biosynthesis; Transferase; Transit peptide.
FT TRANSIT 1..70
FT /note="Chloroplast"
FT /evidence="ECO:0000269|PubMed:12226499"
FT CHAIN 71..603
FT /note="Granule-bound starch synthase 1,
FT chloroplastic/amyloplastic"
FT /id="PRO_0000011126"
FT BINDING 90
FT /ligand="ADP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:57498"
FT /evidence="ECO:0000250"
FT VARIANT 9
FT /note="L -> F (in strain: cv. Shikoku Hadaka 84)"
FT VARIANT 21
FT /note="R -> RSAPSM (in strain: cv. Iyatoma Mochi, cv.
FT Mochimugi-D, cv. Shikoku Hadaka 84, cv. Waxy Oderbrucker
FT and cv. SB 85750)"
FT VARIANT 24..25
FT /note="RP -> HA (in strain: cv. Iyatoma Mochi, cv.
FT Mochimugi-D, cv. Shikoku Hadaka 84, cv. Waxy Oderbrucker
FT and cv. SB 85750)"
FT VARIANT 34
FT /note="N -> K (in strain: cv. Iyatoma Mochi, cv. Mochimugi-
FT D, cv. Shikoku Hadaka 84, cv. Waxy Oderbrucker and cv. SB
FT 85750)"
FT VARIANT 38..40
FT /note="AAL -> GTF (in strain: cv. Iyatoma Mochi, cv.
FT Mochimugi-D, cv. Shikoku Hadaka 84, cv. Waxy Oderbrucker
FT and cv. SB 85750)"
FT VARIANT 45
FT /note="I -> V (in strain: cv. Iyatoma Mochi, cv. Mochimugi-
FT D, cv. Shikoku Hadaka 84, cv. Waxy Oderbrucker and cv. SB
FT 85750)"
FT VARIANT 61
FT /note="S -> N (in strain: cv. Iyatoma Mochi, cv. Mochimugi-
FT D, cv. Shikoku Hadaka 84, cv. Waxy Oderbrucker and cv. SB
FT 85750)"
FT VARIANT 70
FT /note="S -> R (in strain: cv. Iyatoma Mochi, cv. Morex, cv.
FT Mochimugi-D, cv. Shikoku Hadaka 84, cv. Waxy Oderbrucker
FT and cv. SB 85750)"
FT VARIANT 287
FT /note="D -> V (in strain: cv. CDC Alamo)"
FT VARIANT 391
FT /note="R -> W (in strain: cv. Iyatoma Mochi)"
FT VARIANT 414
FT /note="A -> V (in strain: cv. Iyatoma Mochi)"
FT VARIANT 513
FT /note="G -> W (in strain: cv. Iyatoma Mochi, cv. Waxy
FT Oderbrucker and cv. SB 85750)"
FT VARIANT 558..559
FT /note="MI -> VV (in strain: cv. CDC Alamo)"
SQ SEQUENCE 603 AA; 66211 MW; 0B0B3DE6A8217934 CRC64;
MAALATSQLA TSGTVLGVTD RFRRPGFQGL RPRNPADAAL GMRTIGASAA PKQSRKAHRG
SRRCLSVVVS ATGSGMNLVF VGAEMAPWSK TGGLGDVLGG LPPAMAANGH RVMVVSPRYD
QYKDAWDTSV ISEIKVADEY ERVRFFHCYK RGVDRVFIDH PWFLEKVRGK TKEKIYGPDA
GTDYEDNQQR FSLLCQAALE APRILNLNNN PYFSGPYGED VVFVCNDWHT GLLACYLKSN
YQSNGIYRTA KVAFCIHNIS YQGRFSFDDF AQLNLPDRFK SSFDFIDGYD KPVEGRKINW
MKAGILQADK VLTVSPYYAE ELISGEARGC ELDNIMRLTG ITGIVNGMDV SEWDPTKDKF
LAVNYDITTA LEAKALNKEA LQAEVGLPVD RKVPLVAFIG RLEEQKGPDV MIAAIPEILK
EEDVQIILLG TGKKKFEKLL KSMEEKFPGK VRAVVRFNAP LAHQMMAGAD LLAVTSRFEP
CGLIQLQGMR YGTPCVCAST GGLVDTIVEG KTGFHMGRLS VDCNVVEPAD VKKVATTLKR
AVKVVGTPAY QEMVKNCMIQ DLSWKGPAKN WEDVLLELGV EGSEPGIVGE EIAPLAMENV
AAP
