SSG1_ORYSI
ID SSG1_ORYSI Reviewed; 609 AA.
AC A2Y8X2; P19395; Q1ZZT5; Q43012; Q43013; Q71F57; Q8GZD6; Q8S9C4; Q94LY7;
AC Q9S7R1; Q9S7U4;
DT 24-JUL-2007, integrated into UniProtKB/Swiss-Prot.
DT 24-JUL-2007, sequence version 2.
DT 03-AUG-2022, entry version 79.
DE RecName: Full=Granule-bound starch synthase 1, chloroplastic/amyloplastic;
DE EC=2.4.1.242;
DE AltName: Full=Granule-bound starch synthase I;
DE Short=GBSS-I;
DE AltName: Allergen=Ory s GBSS_I;
DE Flags: Precursor;
GN Name=WAXY; Synonyms=WX, WX-B; ORFNames=OsI_020765;
OS Oryza sativa subsp. indica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39946;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA Wang Z.Y., Zheng F.Q., Gao J.P., Wang X.Q., Wu M., Zhang J.L., Hong M.M.;
RT "Identification of two transposon-like elements in rice Wx gene.";
RL Sci. China, Ser. B, Chem. Life Sci. Earth Sci. 37:437-447(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=cv. Haomuxi;
RA Sun H.Q., Luo K.;
RL Submitted (FEB-2006) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. 93-11;
RX PubMed=15685292; DOI=10.1371/journal.pbio.0030038;
RA Yu J., Wang J., Lin W., Li S., Li H., Zhou J., Ni P., Dong W., Hu S.,
RA Zeng C., Zhang J., Zhang Y., Li R., Xu Z., Li S., Li X., Zheng H., Cong L.,
RA Lin L., Yin J., Geng J., Li G., Shi J., Liu J., Lv H., Li J., Wang J.,
RA Deng Y., Ran L., Shi X., Wang X., Wu Q., Li C., Ren X., Wang J., Wang X.,
RA Li D., Liu D., Zhang X., Ji Z., Zhao W., Sun Y., Zhang Z., Bao J., Han Y.,
RA Dong L., Ji J., Chen P., Wu S., Liu J., Xiao Y., Bu D., Tan J., Yang L.,
RA Ye C., Zhang J., Xu J., Zhou Y., Yu Y., Zhang B., Zhuang S., Wei H.,
RA Liu B., Lei M., Yu H., Li Y., Xu H., Wei S., He X., Fang L., Zhang Z.,
RA Zhang Y., Huang X., Su Z., Tong W., Li J., Tong Z., Li S., Ye J., Wang L.,
RA Fang L., Lei T., Chen C.-S., Chen H.-C., Xu Z., Li H., Huang H., Zhang F.,
RA Xu H., Li N., Zhao C., Li S., Dong L., Huang Y., Li L., Xi Y., Qi Q.,
RA Li W., Zhang B., Hu W., Zhang Y., Tian X., Jiao Y., Liang X., Jin J.,
RA Gao L., Zheng W., Hao B., Liu S.-M., Wang W., Yuan L., Cao M.,
RA McDermott J., Samudrala R., Wang J., Wong G.K.-S., Yang H.;
RT "The genomes of Oryza sativa: a history of duplications.";
RL PLoS Biol. 3:266-281(2005).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY, AND ALLERGEN.
RX PubMed=24016103; DOI=10.1021/jf402759f;
RA Golias J., Humlova Z., Halada P., Habova V., Janatkova I., Tuckova L.;
RT "Identification of rice proteins recognized by the IgE antibodies of
RT patients with food allergies.";
RL J. Agric. Food Chem. 61:8851-8860(2013).
CC -!- FUNCTION: Required for the synthesis of amylose in endosperm.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[(1->4)-alpha-D-glucosyl](n) + an NDP-alpha-D-glucose =
CC [(1->4)-alpha-D-glucosyl](n+1) + a ribonucleoside 5'-diphosphate +
CC H(+); Xref=Rhea:RHEA:15873, Rhea:RHEA-COMP:9584, Rhea:RHEA-COMP:9587,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15444, ChEBI:CHEBI:57930,
CC ChEBI:CHEBI:76533; EC=2.4.1.242;
CC -!- PATHWAY: Glycan biosynthesis; starch biosynthesis.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast. Plastid, amyloplast.
CC Note=Amyloplast or chloroplast, granule-bound. {ECO:0000250}.
CC -!- ALLERGEN: Causes an allergic reaction in human. Binds to IgE.
CC {ECO:0000269|PubMed:24016103}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 1 family.
CC Bacterial/plant glycogen synthase subfamily. {ECO:0000305}.
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DR EMBL; X65183; CAA46294.1; -; Genomic_DNA.
DR EMBL; DQ415640; ABD77490.1; -; Genomic_DNA.
DR EMBL; CM000131; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; A2Y8X2; -.
DR SMR; A2Y8X2; -.
DR STRING; 39946.A2Y8X2; -.
DR Allergome; 11008; Ory s GBSS_I.
DR CAZy; GT5; Glycosyltransferase Family 5.
DR EnsemblPlants; BGIOSGA022241-TA; BGIOSGA022241-PA; BGIOSGA022241.
DR Gramene; BGIOSGA022241-TA; BGIOSGA022241-PA; BGIOSGA022241.
DR HOGENOM; CLU_009583_18_2_1; -.
DR OMA; EMGPAKN; -.
DR UniPathway; UPA00152; -.
DR Proteomes; UP000007015; Chromosome 6.
DR ExpressionAtlas; A2Y8X2; differential.
DR GO; GO:0009501; C:amyloplast; IEA:UniProtKB-SubCell.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0043531; F:ADP binding; IEA:EnsemblPlants.
DR GO; GO:0102502; F:ADP-glucose-starch glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0004373; F:glycogen (starch) synthase activity; IEA:InterPro.
DR GO; GO:0019863; F:IgE binding; IDA:UniProtKB.
DR GO; GO:0019252; P:starch biosynthetic process; IEA:UniProtKB-UniPathway.
DR HAMAP; MF_00484; Glycogen_synth; 1.
DR InterPro; IPR001296; Glyco_trans_1.
DR InterPro; IPR011835; GS/SS.
DR InterPro; IPR013534; Starch_synth_cat_dom.
DR Pfam; PF08323; Glyco_transf_5; 1.
DR Pfam; PF00534; Glycos_transf_1; 1.
DR TIGRFAMs; TIGR02095; glgA; 1.
PE 1: Evidence at protein level;
KW Allergen; Amyloplast; Chloroplast; Disulfide bond; Glycosyltransferase;
KW Plastid; Reference proteome; Starch biosynthesis; Transferase;
KW Transit peptide.
FT TRANSIT 1..77
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 78..609
FT /note="Granule-bound starch synthase 1,
FT chloroplastic/amyloplastic"
FT /id="PRO_0000295649"
FT REGION 29..67
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 44..67
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 97
FT /ligand="ADP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:57498"
FT /evidence="ECO:0000250|UniProtKB:P0A6U8"
FT BINDING 100
FT /ligand="ADP"
FT /ligand_id="ChEBI:CHEBI:456216"
FT /evidence="ECO:0000250|UniProtKB:Q0DEV5"
FT BINDING 408
FT /ligand="ADP"
FT /ligand_id="ChEBI:CHEBI:456216"
FT /evidence="ECO:0000250|UniProtKB:Q0DEV5"
FT BINDING 413
FT /ligand="ADP"
FT /ligand_id="ChEBI:CHEBI:456216"
FT /evidence="ECO:0000250|UniProtKB:Q0DEV5"
FT BINDING 462
FT /ligand="ADP"
FT /ligand_id="ChEBI:CHEBI:456216"
FT /evidence="ECO:0000250|UniProtKB:Q0DEV5"
FT BINDING 493
FT /ligand="ADP"
FT /ligand_id="ChEBI:CHEBI:456216"
FT /evidence="ECO:0000250|UniProtKB:Q0DEV5"
FT DISULFID 337..529
FT /evidence="ECO:0000250|UniProtKB:Q0DEV5"
FT VARIANT 415
FT /note="P -> S (in strain: cv. Haomuxi)"
FT CONFLICT 166
FT /note="D -> G (in Ref. 2; ABD77490)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 609 AA; 66476 MW; C225DBF6F12072C5 CRC64;
MSALTTSQLA TSATGFGIAD RSAPSSLLRH GFQGLKPRSP AGGDATSLSV TTSARATPKQ
QRSVQRGSRR FPSVVVYATG AGMNVVFVGA EMAPWSKTGG LGDVLGGLPP AMAANGHRVM
VISPRYDQYK DAWDTSVVAE IKVADRYERV RFFHCYKRGV DRVFIDHPSF LEKVWGKTGE
KIYGPDTGVD YKDNQMRFSL LCQAALEAPR ILNLNNNPYF KGTYGEDVVF VCNDWHTGPL
ASYLKNNYQP NGIYRNAKVA FCIHNISYQG RFAFEDYPEL NLSERFRSSF DFIDGYDTPV
EGRKINWMKA GILEADRVLT VSPYYAEELI SGIARGCELD NIMRLTGITG IVNGMDVSEW
DPSKDKYITA KYDATTAIEA KALNKEALQA EAGLPVDRKI PLIAFIGRLE EQKGPDVMAA
AIPELMQEDV QIVLLGTGKK KFEKLLKSME EKYPGKVRAV VKFNAPLAHL IMAGADVLAV
PSRFEPCGLI QLQGMRYGTP CACASTGGLV DTVIEGKTGF HMGRLSVDCK VVEPSDVKKV
AATLKRAIKV VGTPAYEEMV RNCMNQDLSW KGPAKNWENV LLGLGVAGSA PGIEGDEIAP
LAKENVAAP
