ID   SSG1_SOLTU              Reviewed;         607 AA.
AC   Q00775; Q43176;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1993, sequence version 1.
DT   03-AUG-2022, entry version 117.
DE   RecName: Full=Granule-bound starch synthase 1, chloroplastic/amyloplastic;
DE            EC=2.4.1.242;
DE   AltName: Full=Granule-bound starch synthase I;
DE            Short=GBSS-I;
DE   Flags: Precursor;
GN   Name=WAXY; Synonyms=GBSS;
OS   Solanum tuberosum (Potato).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   asterids; lamiids; Solanales; Solanaceae; Solanoideae; Solaneae; Solanum.
OX   NCBI_TaxID=4113;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=cv. AM79.7322;
RX   PubMed=1886609; DOI=10.1007/bf00282472;
RA   van der Leij F.R., Visser R.G.F., Ponstein A.S., Jacobsen E.,
RA   Feenstra W.J.;
RT   "Sequence of the structural gene for granule-bound starch synthase of
RT   potato (Solanum tuberosum L.) and evidence for a single point deletion in
RT   the amf allele.";
RL   Mol. Gen. Genet. 228:240-248(1991).
RN   [2]
RP   SEQUENCE REVISION.
RA   van der Leij F.R.;
RL   Submitted (JUN-1992) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Dongnong 303;
RA   Dai W.L., Deng W., Cui M., Xiu M., Zhao S.Y., Wang X.M.;
RL   Submitted (DEC-1994) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. DM1-3 516 R44;
RX   PubMed=21743474; DOI=10.1038/nature10158;
RG   The Potato Genome Sequencing Consortium;
RT   "Genome sequence and analysis of the tuber crop potato.";
RL   Nature 475:189-195(2011).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[(1->4)-alpha-D-glucosyl](n) + an NDP-alpha-D-glucose =
CC         [(1->4)-alpha-D-glucosyl](n+1) + a ribonucleoside 5'-diphosphate +
CC         H(+); Xref=Rhea:RHEA:15873, Rhea:RHEA-COMP:9584, Rhea:RHEA-COMP:9587,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15444, ChEBI:CHEBI:57930,
CC         ChEBI:CHEBI:76533; EC=2.4.1.242;
CC   -!- PATHWAY: Glycan biosynthesis; starch biosynthesis.
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast. Plastid, amyloplast.
CC       Note=Amyloplast or chloroplast, granule-bound.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 1 family.
CC       Bacterial/plant glycogen synthase subfamily. {ECO:0000305}.
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DR   EMBL; X58453; CAA41359.1; -; Genomic_DNA.
DR   EMBL; X83220; CAA58220.1; -; Genomic_DNA.
DR   PIR; S16555; YUPOY.
DR   RefSeq; XP_006343763.1; XM_006343701.2.
DR   RefSeq; XP_015162572.1; XM_015307086.1.
DR   AlphaFoldDB; Q00775; -.
DR   SMR; Q00775; -.
DR   STRING; 4113.PGSC0003DMT400031568; -.
DR   CAZy; GT5; Glycosyltransferase Family 5.
DR   PRIDE; Q00775; -.
DR   EnsemblPlants; PGSC0003DMT400031568; PGSC0003DMT400031568; PGSC0003DMG400012111.
DR   GeneID; 102577459; -.
DR   Gramene; PGSC0003DMT400031568; PGSC0003DMT400031568; PGSC0003DMG400012111.
DR   eggNOG; ENOG502QQX3; Eukaryota.
DR   InParanoid; Q00775; -.
DR   OMA; CGWVHGR; -.
DR   OrthoDB; 1142473at2759; -.
DR   BRENDA; 2.4.1.242; 5757.
DR   SABIO-RK; Q00775; -.
DR   UniPathway; UPA00152; -.
DR   Proteomes; UP000011115; Unassembled WGS sequence.
DR   ExpressionAtlas; Q00775; baseline.
DR   GO; GO:0009501; C:amyloplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0009569; C:chloroplast starch grain; IEA:EnsemblPlants.
DR   GO; GO:0102502; F:ADP-glucose-starch glucosyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004373; F:glycogen (starch) synthase activity; IEA:InterPro.
DR   GO; GO:0019252; P:starch biosynthetic process; IEA:UniProtKB-UniPathway.
DR   HAMAP; MF_00484; Glycogen_synth; 1.
DR   InterPro; IPR001296; Glyco_trans_1.
DR   InterPro; IPR011835; GS/SS.
DR   InterPro; IPR013534; Starch_synth_cat_dom.
DR   Pfam; PF08323; Glyco_transf_5; 1.
DR   Pfam; PF00534; Glycos_transf_1; 1.
DR   TIGRFAMs; TIGR02095; glgA; 1.
PE   1: Evidence at protein level;
KW   Amyloplast; Chloroplast; Direct protein sequencing; Glycosyltransferase;
KW   Plastid; Reference proteome; Starch biosynthesis; Transferase;
KW   Transit peptide.
FT   TRANSIT         1..77
FT                   /note="Chloroplast"
FT   CHAIN           78..607
FT                   /note="Granule-bound starch synthase 1,
FT                   chloroplastic/amyloplastic"
FT                   /id="PRO_0000011134"
FT   REGION          585..607
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   BINDING         95
FT                   /ligand="ADP-alpha-D-glucose"
FT                   /ligand_id="ChEBI:CHEBI:57498"
FT                   /evidence="ECO:0000250"
FT   CONFLICT        130
FT                   /note="A -> T (in Ref. 3; CAA58220)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        398
FT                   /note="I -> V (in Ref. 3; CAA58220)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   607 AA;  66576 MW;  2A377865CFAFA650 CRC64;
     MASITASHHF VSRSQTSLDT KSTLSQIGLR NHTLTHNGLR AVNKLDGLQS RTNTKVTPKM
     ASRTETKRPG CSATIVCGKG MNLIFVGTEV GPWSKTGGLG DVLGGLPPAL AARGHRVMTI
     SPRYDQYKDA WDTSVAVEVK VGDSIEIVRF FHCYKRGVDR VFVDHPMFLE KVWGKTGSKI
     YGPKAGLDYL DNELRFSLLC QAALEAPKVL NLNSSNYFSG PYGEDVLFIA NDWHTALIPC
     YLKSMYQSRG IYLNAKVAFC IHNIAYQGRF SFSDFPLLNL PDEFRGSFDF IDGYEKPVKG
     RKINWMKAGI LESHRVVTVS PYYAQELVSA VDKGVELDSV LRKTCITGIV NGMDTQEWNP
     ATDKYTDVKY DITTVMDAKP LLKEALQAAV GLPVDKKIPL IGFIGRLEEQ KGSDILVAAI
     HKFIGLDVQI VVLGTGKKEF EQEIEQLEVL YPNKAKGVAK FNVPLAHMIT AGADFMLVPS
     RFEPCGLIQL HAMRYGTVPI CASTGGLVDT VKEGYTGFHM GAFNVECDVV DPADVLKIVT
     TVARALAVYG TLAFAEMIKN CMSEELSWKE PAKKWETLLL GLGASGSEPG VEGEEIAPLA
     KENVATP