SSG1_WHEAT
ID SSG1_WHEAT Reviewed; 615 AA.
AC P27736;
DT 01-AUG-1992, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1992, sequence version 1.
DT 03-AUG-2022, entry version 100.
DE RecName: Full=Granule-bound starch synthase 1, chloroplastic/amyloplastic;
DE EC=2.4.1.242;
DE AltName: Full=Granule-bound starch synthase I;
DE Short=GBSS-I;
DE Flags: Precursor;
GN Name=WAXY;
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Endosperm;
RX PubMed=1863765; DOI=10.1007/bf00016086;
RA Clark J.R., Robertson M., Ainsworth C.C.;
RT "Nucleotide sequence of a wheat (Triticum aestivum L.) cDNA clone encoding
RT the waxy protein.";
RL Plant Mol. Biol. 16:1099-1101(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], AND TISSUE SPECIFICITY.
RC STRAIN=cv. Florida; TISSUE=Kernel;
RX PubMed=12081374; DOI=10.1023/a:1015576930688;
RA Kluth A., Sprunck S., Becker D., Loerz H., Luetticke S.;
RT "5' deletion of a gbss1 promoter region from wheat leads to changes in
RT tissue and developmental specificities.";
RL Plant Mol. Biol. 49:669-682(2002).
RN [3]
RP PROTEIN SEQUENCE OF 71-78.
RC STRAIN=cv. Chinese Spring;
RX PubMed=8499619; DOI=10.1007/bf00038996;
RA Ainsworth C., Clark J., Balsdon J.;
RT "Expression, organisation and structure of the genes encoding the waxy
RT protein (granule-bound starch synthase) in wheat.";
RL Plant Mol. Biol. 22:67-82(1993).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=[(1->4)-alpha-D-glucosyl](n) + an NDP-alpha-D-glucose =
CC [(1->4)-alpha-D-glucosyl](n+1) + a ribonucleoside 5'-diphosphate +
CC H(+); Xref=Rhea:RHEA:15873, Rhea:RHEA-COMP:9584, Rhea:RHEA-COMP:9587,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15444, ChEBI:CHEBI:57930,
CC ChEBI:CHEBI:76533; EC=2.4.1.242;
CC -!- PATHWAY: Glycan biosynthesis; starch biosynthesis.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast. Plastid, amyloplast.
CC Note=Amyloplast or chloroplast, granule-bound.
CC -!- TISSUE SPECIFICITY: Found in seeds and pollen.
CC {ECO:0000269|PubMed:12081374}.
CC -!- DEVELOPMENTAL STAGE: Expressed during the grain filling process.
CC -!- SIMILARITY: Belongs to the glycosyltransferase 1 family.
CC Bacterial/plant glycogen synthase subfamily. {ECO:0000305}.
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DR EMBL; X57233; CAA40509.1; -; mRNA.
DR EMBL; AY050174; AAL05405.1; -; mRNA.
DR PIR; S16261; YUWTY.
DR AlphaFoldDB; P27736; -.
DR SMR; P27736; -.
DR STRING; 4565.Traes_4AL_4B9D56131.3; -.
DR CAZy; GT5; Glycosyltransferase Family 5.
DR PRIDE; P27736; -.
DR eggNOG; ENOG502QQX3; Eukaryota.
DR BRENDA; 2.4.1.242; 6500.
DR UniPathway; UPA00152; -.
DR Proteomes; UP000019116; Unplaced.
DR ExpressionAtlas; P27736; baseline and differential.
DR GO; GO:0009501; C:amyloplast; IEA:UniProtKB-SubCell.
DR GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR GO; GO:0102502; F:ADP-glucose-starch glucosyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0004373; F:glycogen (starch) synthase activity; IEA:InterPro.
DR GO; GO:0019252; P:starch biosynthetic process; IEA:UniProtKB-UniPathway.
DR HAMAP; MF_00484; Glycogen_synth; 1.
DR InterPro; IPR001296; Glyco_trans_1.
DR InterPro; IPR011835; GS/SS.
DR InterPro; IPR013534; Starch_synth_cat_dom.
DR Pfam; PF08323; Glyco_transf_5; 1.
DR Pfam; PF00534; Glycos_transf_1; 1.
DR TIGRFAMs; TIGR02095; glgA; 1.
PE 1: Evidence at protein level;
KW Amyloplast; Chloroplast; Direct protein sequencing; Glycosyltransferase;
KW Plastid; Reference proteome; Starch biosynthesis; Transferase;
KW Transit peptide.
FT TRANSIT 1..70
FT /note="Chloroplast"
FT /evidence="ECO:0000269|PubMed:8499619"
FT CHAIN 71..615
FT /note="Granule-bound starch synthase 1,
FT chloroplastic/amyloplastic"
FT /id="PRO_0000011136"
FT BINDING 91
FT /ligand="ADP-alpha-D-glucose"
FT /ligand_id="ChEBI:CHEBI:57498"
FT /evidence="ECO:0000250"
SQ SEQUENCE 615 AA; 67751 MW; B7D15F5E1D41D159 CRC64;
MAALVTSQLA TSGTVLSVTD RFRRPGFQGL RPRNPADAAL GMRTVGASAA PKQSRKPHRF
DRRCLSMVVR ATGSGGMNLV FVGAEMAPWS KTGGLGDVLG GLPAAMAANG HRVMVISPRY
DQYKDAWDTS VISEIKVVDR YERVRYFHCY KRGVDRVFVD HPCFLEKVRG KTKEKIYGPD
AGTDYEDNQQ RFSLLCQAAL EVPRILDLNN NPHFSGPYAM LCRAVPRRAG EDVVFVCNDW
HTGLLACYLK SNYQSNGIYR TAKVAFCIHN ISYQGRFSFD DFAQLNLPDR FKSSFDFIDG
YDKPVEGRKI NWMKAGILQA DKVLTVSPYY AEELISGEAR GCELDNIMRL TGITGIVNGM
DVSEWDPIKD KFLTVNYDVT TALEGKALNK EALQAEVGLP VDRKVPLVAF IGRLEEQKGP
DVMIAAIPEI VKEEDVQIVL LGTGKKKFER LLKSVEEKFP TKVRAVVRFN APLAHQMMAG
ADVLAVTSRF EPCGLIQLQG MRYGTPCACA STGGLVDTIV EGKTGFHMGR LSVDCNVVEP
ADVKKVVTTL KRAVKVVGTP AYHEMVKNCM IQDLSWKGPA KNWEDVLLEL GVEGSEPGIV
GEEIAPLALE NVAAP
