ID   SSG2_AVESA              Reviewed;         518 AA.
AC   P14812;
DT   01-APR-1990, integrated into UniProtKB/Swiss-Prot.
DT   01-APR-1990, sequence version 1.
DT   25-MAY-2022, entry version 89.
DE   RecName: Full=12S seed storage globulin 2;
DE   Contains:
DE     RecName: Full=12S seed storage globulin 2 acidic chain;
DE   Contains:
DE     RecName: Full=12S seed storage globulin 2 basic chain;
DE   Flags: Precursor;
OS   Avena sativa (Oat).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC   Pooideae; Poodae; Poeae; Aveninae; Avena.
OX   NCBI_TaxID=4498;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=cv. Solidor; TISSUE=Leaf;
RX   PubMed=2326176; DOI=10.1093/nar/18.2.377;
RA   Schubert R., Baeumlein H., Czihal A., Wobus U.;
RT   "Genomic sequence of a 12S seed storage protein gene from oat (Avena sativa
RT   L. cv. 'Solidor').";
RL   Nucleic Acids Res. 18:377-377(1990).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2351662; DOI=10.1016/s0021-9258(19)38719-8;
RA   Shotwell M.A., Boyer S.K., Chesnut R.S., Larkins B.A.;
RT   "Analysis of seed storage protein genes of oats.";
RL   J. Biol. Chem. 265:9652-9658(1990).
CC   -!- FUNCTION: This is a seed storage protein.
CC   -!- SUBUNIT: Hexamer; each subunit is composed of an acidic and a basic
CC       chain derived from a single precursor and linked by a disulfide bond.
CC   -!- SIMILARITY: Belongs to the 11S seed storage protein (globulins) family.
CC       {ECO:0000305}.
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DR   EMBL; X17637; CAA35631.1; -; Genomic_DNA.
DR   EMBL; J05485; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   PIR; A36433; A36433.
DR   PIR; S07897; S07897.
DR   AlphaFoldDB; P14812; -.
DR   SMR; P14812; -.
DR   Allergome; 10849; Ave s 11S.
DR   GO; GO:0045735; F:nutrient reservoir activity; IEA:UniProtKB-KW.
DR   Gene3D; 2.60.120.10; -; 2.
DR   InterPro; IPR022379; 11S_seedstore_CS.
DR   InterPro; IPR006044; 11S_seedstore_pln.
DR   InterPro; IPR006045; Cupin_1.
DR   InterPro; IPR014710; RmlC-like_jellyroll.
DR   InterPro; IPR011051; RmlC_Cupin_sf.
DR   Pfam; PF00190; Cupin_1; 2.
DR   PRINTS; PR00439; 11SGLOBULIN.
DR   SMART; SM00835; Cupin_1; 2.
DR   SUPFAM; SSF51182; SSF51182; 1.
DR   PROSITE; PS00305; 11S_SEED_STORAGE; 1.
PE   3: Inferred from homology;
KW   Disulfide bond; Seed storage protein; Signal; Storage protein.
FT   SIGNAL          1..24
FT   CHAIN           25..317
FT                   /note="12S seed storage globulin 2 acidic chain"
FT                   /id="PRO_0000032086"
FT   CHAIN           318..518
FT                   /note="12S seed storage globulin 2 basic chain"
FT                   /id="PRO_0000032087"
FT   DOMAIN          50..240
FT                   /note="Cupin type-1 1"
FT                   /evidence="ECO:0000255"
FT   DOMAIN          330..479
FT                   /note="Cupin type-1 2"
FT                   /evidence="ECO:0000255"
FT   REGION          280..311
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          482..518
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        494..510
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   DISULFID        45..78
FT                   /evidence="ECO:0000250"
FT   DISULFID        121..324
FT                   /note="Interchain (between acidic and basic chains)"
FT                   /evidence="ECO:0000255"
FT   CONFLICT        60
FT                   /note="R -> G (in Ref. 2)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   518 AA;  58676 MW;  82B3037D43797ADE CRC64;
     MATTRFPSLL FYSYIFLLCN GSMAQLFGQS FTPWQSSRQG GLRGCRFDRL QAFEPLRQVR
     SQAGIIEYFD EQNEQFRCAG VSVIRRVIEP QGLLLPQYHN APGLVYILQG RGFTGLTFPG
     CPATFQQQFQ PFDQAQFAEG QSQSQNLKDE HQRVHHIKQG DVVALPAGIV HWCYNDGDAP
     IVAVYVFDVN NNANQLEPRQ KEFLLAGNNK REQQFGQNIF SGFSVQLLSE ALGISQQVAQ
     KIQSQNDQRG EIIRVSQGLQ FLKPFVSQQG PVEHQAYQPI QSQEEQSTQY QVGQSPQYQE
     GQSTQYQPGQ SWDQSFNGLE ENFCSLEARQ NIENPKRADT YNPRAGRITH LNSKNFPTLN
     LVQMSATRVN LYQNAILSPY WNINAHSVMH MIQGRARVQV VNNHGQTVFN DILRRGQLLI
     IPQHYVVLKK AEREGCQYIS FKTNPNSMVS QIAGKTSILR ALPVDVLANA YRISRQEAQN
     LKNNRGEEFD AFTPKFTQTG SQSYQDEGES SSTEKASE