SSG4_ORYSJ
ID SSG4_ORYSJ Reviewed; 2135 AA.
AC W0RYD3; Q0JQ65; Q5VRT8;
DT 05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT 19-MAR-2014, sequence version 1.
DT 03-AUG-2022, entry version 37.
DE RecName: Full=Protein SUBSTANDARD STARCH GRAIN 4, chloroplastic {ECO:0000303|PubMed:24335509};
DE AltName: Full=Translocon at the inner-envelope- membrane of chloroplasts SSG4 {ECO:0000305};
DE Flags: Precursor;
GN Name=SSG4 {ECO:0000303|PubMed:24335509};
GN OrderedLocusNames=Os01g0179400 {ECO:0000312|EMBL:BAS70711.1},
GN LOC_Os01g08420 {ECO:0000305};
GN ORFNames=P0406H10.19 {ECO:0000312|EMBL:BAD67835.1};
OS Oryza sativa subsp. japonica (Rice).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Oryzoideae; Oryzeae; Oryzinae; Oryza; Oryza sativa.
OX NCBI_TaxID=39947;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, AND MUTAGENESIS OF GLY-1924.
RX PubMed=24335509; DOI=10.1104/pp.113.229591;
RA Matsushima R., Maekawa M., Kusano M., Kondo H., Fujita N., Kawagoe Y.,
RA Sakamoto W.;
RT "Amyloplast-localized SUBSTANDARD STARCH GRAIN4 protein influences the size
RT of starch grains in rice endosperm.";
RL Plant Physiol. 164:623-636(2014).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=12447438; DOI=10.1038/nature01184;
RA Sasaki T., Matsumoto T., Yamamoto K., Sakata K., Baba T., Katayose Y.,
RA Wu J., Niimura Y., Cheng Z., Nagamura Y., Antonio B.A., Kanamori H.,
RA Hosokawa S., Masukawa M., Arikawa K., Chiden Y., Hayashi M., Okamoto M.,
RA Ando T., Aoki H., Arita K., Hamada M., Harada C., Hijishita S., Honda M.,
RA Ichikawa Y., Idonuma A., Iijima M., Ikeda M., Ikeno M., Ito S., Ito T.,
RA Ito Y., Ito Y., Iwabuchi A., Kamiya K., Karasawa W., Katagiri S.,
RA Kikuta A., Kobayashi N., Kono I., Machita K., Maehara T., Mizuno H.,
RA Mizubayashi T., Mukai Y., Nagasaki H., Nakashima M., Nakama Y.,
RA Nakamichi Y., Nakamura M., Namiki N., Negishi M., Ohta I., Ono N., Saji S.,
RA Sakai K., Shibata M., Shimokawa T., Shomura A., Song J., Takazaki Y.,
RA Terasawa K., Tsuji K., Waki K., Yamagata H., Yamane H., Yoshiki S.,
RA Yoshihara R., Yukawa K., Zhong H., Iwama H., Endo T., Ito H., Hahn J.H.,
RA Kim H.-I., Eun M.-Y., Yano M., Jiang J., Gojobori T.;
RT "The genome sequence and structure of rice chromosome 1.";
RL Nature 420:312-316(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Nipponbare;
RX PubMed=16100779; DOI=10.1038/nature03895;
RG International rice genome sequencing project (IRGSP);
RT "The map-based sequence of the rice genome.";
RL Nature 436:793-800(2005).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=18089549; DOI=10.1093/nar/gkm978;
RG The rice annotation project (RAP);
RT "The rice annotation project database (RAP-DB): 2008 update.";
RL Nucleic Acids Res. 36:D1028-D1033(2008).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Nipponbare;
RX PubMed=24280374; DOI=10.1186/1939-8433-6-4;
RA Kawahara Y., de la Bastide M., Hamilton J.P., Kanamori H., McCombie W.R.,
RA Ouyang S., Schwartz D.C., Tanaka T., Wu J., Zhou S., Childs K.L.,
RA Davidson R.M., Lin H., Quesada-Ocampo L., Vaillancourt B., Sakai H.,
RA Lee S.S., Kim J., Numa H., Itoh T., Buell C.R., Matsumoto T.;
RT "Improvement of the Oryza sativa Nipponbare reference genome using next
RT generation sequence and optical map data.";
RL Rice 6:4-4(2013).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 1107-2135.
RC STRAIN=cv. Nipponbare;
RX PubMed=12869764; DOI=10.1126/science.1081288;
RG The rice full-length cDNA consortium;
RT "Collection, mapping, and annotation of over 28,000 cDNA clones from
RT japonica rice.";
RL Science 301:376-379(2003).
CC -!- FUNCTION: Part of the inner chloroplast membrane translocon complex
CC (TIC) which associates with the outer chloroplast membrane translocon
CC complex (TOC) and forms a supercomplex involved in protein precursor
CC import into the chloroplast stroma (By similarity). Required for the
CC regulation of starch granule size in amyloplasts (PubMed:24335509).
CC {ECO:0000250|UniProtKB:F4ISL7, ECO:0000269|PubMed:24335509}.
CC -!- SUBUNIT: Part of the TIC complex, which can interact with components of
CC the TOC complex to form a larger import complex.
CC {ECO:0000250|UniProtKB:F4ISL7}.
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast inner membrane
CC {ECO:0000250|UniProtKB:F4ISL7}; Single-pass membrane protein
CC {ECO:0000255}. Plastid, chloroplast intermembrane space
CC {ECO:0000250|UniProtKB:F4ISL7}. Plastid, chloroplast
CC {ECO:0000269|PubMed:24335509}. Plastid, amyloplast
CC {ECO:0000269|PubMed:24335509}.
CC -!- TISSUE SPECIFICITY: Highly expressed in third leaf and developing seeds
CC (PubMed:24335509). Expressed in anthers, pistils, flag leaves and young
CC panicles (PubMed:24335509). {ECO:0000269|PubMed:24335509}.
CC -!- SIMILARITY: Belongs to the TamB family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAD67835.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAF04113.2; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
CC Sequence=BAG88743.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB856288; BAO23504.1; -; mRNA.
DR EMBL; AP002524; BAD67835.1; ALT_SEQ; Genomic_DNA.
DR EMBL; AP008207; BAF04113.2; ALT_SEQ; Genomic_DNA.
DR EMBL; AP014957; BAS70711.1; -; Genomic_DNA.
DR EMBL; AK063507; BAG88743.1; ALT_INIT; mRNA.
DR RefSeq; XP_015619226.1; XM_015763740.1.
DR AlphaFoldDB; W0RYD3; -.
DR STRING; 4530.OS01T0179400-01; -.
DR PaxDb; W0RYD3; -.
DR PRIDE; W0RYD3; -.
DR EnsemblPlants; Os01t0179400-01; Os01t0179400-01; Os01g0179400.
DR GeneID; 4323878; -.
DR Gramene; Os01t0179400-01; Os01t0179400-01; Os01g0179400.
DR KEGG; osa:4323878; -.
DR eggNOG; ENOG502QTMX; Eukaryota.
DR HOGENOM; CLU_006278_0_0_1; -.
DR OrthoDB; 35018at2759; -.
DR Proteomes; UP000000763; Chromosome 1.
DR Proteomes; UP000059680; Chromosome 1.
DR GO; GO:0009501; C:amyloplast; IDA:UniProtKB.
DR GO; GO:0009706; C:chloroplast inner membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0031972; C:chloroplast intermembrane space; IEA:UniProtKB-SubCell.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR GO; GO:0009660; P:amyloplast organization; IMP:UniProtKB.
DR GO; GO:0009306; P:protein secretion; IEA:InterPro.
DR InterPro; IPR007452; TamB.
DR Pfam; PF04357; TamB; 1.
PE 1: Evidence at protein level;
KW Amyloplast; Chloroplast; Membrane; Plastid; Plastid inner membrane;
KW Protein transport; Reference proteome; Transit peptide; Transmembrane;
KW Transmembrane helix; Transport.
FT TRANSIT 1..42
FT /note="Chloroplast"
FT /evidence="ECO:0000255"
FT CHAIN 43..2135
FT /note="Protein SUBSTANDARD STARCH GRAIN 4, chloroplastic"
FT /id="PRO_0000447405"
FT TOPO_DOM 43..104
FT /note="Stromal"
FT /evidence="ECO:0000250|UniProtKB:F4ISL7"
FT TRANSMEM 105..125
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 126..2135
FT /note="Chloroplast intermembrane"
FT /evidence="ECO:0000250|UniProtKB:F4ISL7"
FT REGION 1..44
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 58..89
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 361..382
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 401..492
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1843..1869
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 75..89
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 401..422
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 456..490
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 1924
FT /note="G->S: In ssg4; chalky grain with enlarged starch
FT grains in endosperm, but reduced amount of starch in seeds;
FT variegated leaves with increased size of chloroplasts."
FT /evidence="ECO:0000269|PubMed:24335509"
SQ SEQUENCE 2135 AA; 235113 MW; 75631B5D19A4D214 CRC64;
MSHCLRASPF LSPPPPLLHP SRRRRHRQGG CIHTSPGTRP LVARARFDPP PLLRLKVSDS
SDCPAPHHPH SQHQPLLPTR RQQQQPPPPY QALVASLAPL WREGLFLVRC SVFAAALSVA
AALSWYAQLR ARSFVESRLL PAACAALGEF LQREVHLGRV RSVSPLGITL HTCSIGPHAE
EFSCAEVPVM KIRVRPFASL RRGRVVVDAV LSEPSALVAQ RKDFSWLGLP APSEGSPKRH
SGEEGIDYRT KTRRLAREKA AEQWNEERDK AAREAAEMGY IVPSAQSISP SIDEMMEDDG
PVDTGKSSPH LCPDEMHRKD HHIDAGIDSS SKHADLEKSF GVKARIPGIS FWSRMIPNPS
RRRYRRKAHS KLISDTDNSS QQRILRRSAY AAVAYFQNEC SGNPDDSLPG PGESSSDGGH
TNGGGEEGSP NDGPTEYSET TSMDYGELPP EKSNFASTML IGNTDVLNGS SHNQQPSQIS
SHSWENNEQV SEAPVLKKRK NISEDDYRQE FDFGAFGSCT YAHNWLSFWP FQLKGFPVGF
NAPSASLNVQ IQKLRSLFAI GPGDNSAELS QGVGQIHPGA VQQTLPITLD SVYFNGGNLM
LLGYGDQEPR EMKHANGHIK FKNSYNRVHV HVTGNCMEWR QDRTSQGGGY LSTDVFVDIA
EQTWHANLNV VNAFAPLFER ILEIPVVWNK GRATGEVHLC MSKGDSFPSI HGQLDVKGLA
FQILDAPSSF SDIVATLSFR GQRVFLHNAS GWFGDAPVEA SGDFGLNPED GEFHLMCQVP
SVEVNALMKT MKMRPLMFPL AGAVTAVFNC QGPLDAPVFV GSGIVSRKSL SVSGMLPSAA
SEAVMQNKES GAVAAFDHIP FTHVSANFTF NLDNCVADLY GIRACLLDGG EIRGAGNVWI
CPEGEGDDSA MDINLSGSIL LDKVLHRYIP GGIQLIPLKI GELNGETRLS GSLIRPKFDI
KWAAPNAEDS FSDARGNIVI AHDYIMVNSS SVSFDLNTHI QTSYIDDYLL HKEMYQRKKI
MPLIVEGVDL DLRMRGFEFA HIASSIPFDS PRPLHLKASG RFKFQGKVVK YSQLVDEKNH
GAIQGTIDQS KLENDVSRLV GEISLSGIKL NQLMLAPQST GFLSISPDSI MLNATGRPDE
NFSIEVNVPL FFGTHEAIQD GRLLSIFLQK GQLRSNICYH PENLTSLEVR NLPLDELEFA
SLRGFVQKAE LQLNFQKRRG HGLLSVIRPK FSGMLGESLD IAARWSGDVI TMEKSVLEQA
NSKYELQGEY VFPGTRDRFP MESQSNGFIE KAMGGHLGSM MSSMGRWRMR LEVPGAEVAE
MLPLARLLSR STDPAIRSRS KELFMQTLHS VGFNAESLRD QLKALEMYPD WLDDDTIEDI
TLPGLAELRG YWRGSLDASG GGNGDTMADF DFNGEDWEWG TYKTQRVLAS GSFSNNDGLR
LDKLFIQKDN ATLHADGSIL GPLTNLHFAV LNFPVGLIPA LVQAIESSTT DSIHFLRQWL
TPIKGILHME GDLRGTLAKP ECDVQIRLLD GTIGGIDLGR AEVLASVTPT SRFVFDANFE
PTIQSGHVNI QGSVPVTYVD SNSIEEDLEG GDGKQGIIRI PVWAKDRGLT NDISETRIMR
DKPDEGWEFQ LAESLKGLSW NMLEPGEVRI NADIKDGGMT LITALSPYSN WLQGYAEVLL
QVKGTVDHPV VDGSASFHRA TVASPFLRTP LTNFAGNVHV ISNRLCISSM ESRVGRKGRL
SMKGTLPLHN IEPSANDKIE LKCEVLDIRA KNILSGQVDS QLQVTGSILR PDVSGMIRLS
HGEAYLPHDK GNGAVATRLS SNKSISVPAG FDQRTVSRDV SHFLGSLSTS PDGQQSETER
TPEHGSFKPN IDARLNDLKL TFGPELRIVY PLILNFAVSG DLELNGMVHP KYIRPKGVLT
FENGEVNLVA TQVRLKNDHL NVAKFEPDLG LDPILDLVLV GSEWQFKIQS RASMWQDNLV
VTSTRSVDQD VLSPSEAAKV FESQLAESLL EGDGQLAFKK LATATLETLM PRIEGKGEFG
QARWRLVYAP QIPSLLSVDP TVDPLKSLAN NISFATEVEV QLGKRLQASV VRQMKDSEMA
MQWSLIYQLT SRLRVLFQST PSNRLLFEYS ATSQG