ID   SSI12_STRHY             Reviewed;         111 AA.
AC   Q9R641;
DT   24-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-2000, sequence version 1.
DT   25-MAY-2022, entry version 79.
DE   RecName: Full=Subtilisin inhibitor-like protein 12;
DE            Short=SIL-12;
DE            Short=SIL12;
OS   Streptomyces hygroscopicus.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces violaceusniger group.
OX   NCBI_TaxID=1912;
RN   [1]
RP   PROTEIN SEQUENCE, AND CHARACTERIZATION.
RC   STRAIN=ATCC 27438 / DSM 40578 / JCM 4772 / NBRC 13472 / NRRL 2387 /
RC   M5-13184;
RX   PubMed=8597568; DOI=10.1016/0167-4838(95)00207-3;
RA   Terabe M., Kojima S., Taguchi S., Momose H., Miura K.;
RT   "New subtilisin-trypsin inhibitors produced by Streptomyces: primary
RT   structures and their relationship to other proteinase inhibitors from
RT   Streptomyces.";
RL   Biochim. Biophys. Acta 1292:233-240(1996).
CC   -!- FUNCTION: Strong inhibitory activity toward subtilisin BPN' and, to a
CC       lesser extent, toward trypsin.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I16 (SSI) family.
CC       {ECO:0000305}.
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DR   PIR; S65720; S65720.
DR   AlphaFoldDB; Q9R641; -.
DR   SMR; Q9R641; -.
DR   STRING; 68042.GCA_001553435_06428; -.
DR   MEROPS; I16.013; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.350.10; -; 1.
DR   HAMAP; MF_00778; SSI; 1.
DR   InterPro; IPR000691; Prot_inh_I16_SSI.
DR   InterPro; IPR020054; Prot_inh_SSI_I16_CS.
DR   InterPro; IPR023549; Subtilisin_inhibitor.
DR   InterPro; IPR036819; Subtilisin_inhibitor-like_sf.
DR   Pfam; PF00720; SSI; 1.
DR   PRINTS; PR00294; SSBTLNINHBTR.
DR   SUPFAM; SSF55399; SSF55399; 1.
DR   PROSITE; PS00999; SSI; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor; Secreted;
KW   Serine protease inhibitor.
FT   CHAIN           1..111
FT                   /note="Subtilisin inhibitor-like protein 12"
FT                   /id="PRO_0000208663"
FT   SITE            71..72
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000250"
FT   DISULFID        31..46
FT                   /evidence="ECO:0000250"
FT   DISULFID        69..99
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   111 AA;  11696 MW;  608204D09C7D0376 CRC64;
     SLYPASALVL TVGHGADAAT AEVQRAVTLS CRPTPTGTHP APAQACAELH SVGGALGLLR
     TGAEPGRMCT KEWRPITVTA EGVWDGRRVS YEHTFANNCF KNAAPTTVFE F