ID   SSI2_STRRO              Reviewed;         113 AA.
AC   P29607; Q9R2G7;
DT   01-APR-1993, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1994, sequence version 2.
DT   25-MAY-2022, entry version 90.
DE   RecName: Full=Subtilisin inhibitor-like protein 2;
DE            Short=SIL-2;
DE            Short=SIL2;
OS   Streptomyces rochei (Streptomyces parvullus).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces rochei group.
OX   NCBI_TaxID=1928;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=KCC 2283;
RX   PubMed=8143745; DOI=10.1111/j.1432-1033.1994.tb18694.x;
RA   Taguchi S., Kojima S., Terabe M., Miura K., Momose H.;
RT   "Comparative studies on the primary structures and inhibitory properties of
RT   subtilisin-trypsin inhibitors from Streptomyces.";
RL   Eur. J. Biochem. 220:911-918(1994).
RN   [2]
RP   PROTEIN SEQUENCE OF 1-39.
RX   PubMed=1490613; DOI=10.1016/0378-1097(92)90043-n;
RA   Taguchi S., Kojima S., Kumagai I., Ogawara H., Miura K., Momose H.;
RT   "Isolation and partial characterization of SSI-like protease inhibitors
RT   from Streptomyces.";
RL   FEMS Microbiol. Lett. 78:293-297(1992).
RN   [3]
RP   PROTEIN SEQUENCE OF 1-39.
RC   STRAIN=KCC 2283;
RX   PubMed=7763545; DOI=10.1271/bbb.57.522;
RA   Taguchi S., Kikuchi H., Kojima S., Kumagai I., Nakase T., Miura K.,
RA   Momose H.;
RT   "High frequency of SSI-like protease inhibitors among Streptomyces.";
RL   Biosci. Biotechnol. Biochem. 57:522-524(1993).
CC   -!- FUNCTION: Inhibitor of subtilisin BPN' and trypsin.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I16 (SSI) family.
CC       {ECO:0000305}.
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DR   PIR; S42571; S42571.
DR   AlphaFoldDB; P29607; -.
DR   SMR; P29607; -.
DR   MEROPS; I16.007; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.350.10; -; 1.
DR   HAMAP; MF_00778; SSI; 1.
DR   InterPro; IPR000691; Prot_inh_I16_SSI.
DR   InterPro; IPR020054; Prot_inh_SSI_I16_CS.
DR   InterPro; IPR023549; Subtilisin_inhibitor.
DR   InterPro; IPR036819; Subtilisin_inhibitor-like_sf.
DR   Pfam; PF00720; SSI; 1.
DR   PRINTS; PR00294; SSBTLNINHBTR.
DR   SUPFAM; SSF55399; SSF55399; 1.
DR   PROSITE; PS00999; SSI; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor; Secreted;
KW   Serine protease inhibitor.
FT   CHAIN           1..113
FT                   /note="Subtilisin inhibitor-like protein 2"
FT                   /id="PRO_0000208669"
FT   SITE            73..74
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000250"
FT   DISULFID        35..50
FT                   /evidence="ECO:0000250"
FT   DISULFID        71..101
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   113 AA;  11568 MW;  B85B6D10787D40FC CRC64;
     TAPASLYAPS ALVLTIGQGE SAAATSPLRA VTLTCAPKAT GTHPAADAAC AELRRAGGDF
     DALSAADGVM CTREYAPVVV TVDGVWQGRR LSYERTFANE CVKNAGSASV FTF