ID   SSI8_STRVG              Reviewed;         111 AA.
AC   P80388;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1995, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Subtilisin inhibitor-like protein 8;
DE            Short=SIL-8;
DE            Short=SIL8;
GN   Name=sil8;
OS   Streptomyces virginiae.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces virginiae group.
OX   NCBI_TaxID=1961;
RN   [1]
RP   PROTEIN SEQUENCE.
RC   STRAIN=4425;
RX   PubMed=8001578; DOI=10.1111/j.1432-1033.1994.tb20089.x;
RA   Terabe M., Kojima S., Taguchi S., Momose H., Miura K.;
RT   "Primary structure and inhibitory properties of a subtilisin-chymotrypsin
RT   inhibitor from Streptomyces virginiae.";
RL   Eur. J. Biochem. 226:627-632(1994).
CC   -!- FUNCTION: Inhibitor of subtilisin and chymotrypsin.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I16 (SSI) family.
CC       {ECO:0000305}.
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DR   PIR; S51032; S51032.
DR   AlphaFoldDB; P80388; -.
DR   SMR; P80388; -.
DR   STRING; 1961.JOAK01000007_gene4099; -.
DR   MEROPS; I16.005; -.
DR   eggNOG; ENOG50333FU; Bacteria.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.350.10; -; 1.
DR   HAMAP; MF_00778; SSI; 1.
DR   InterPro; IPR000691; Prot_inh_I16_SSI.
DR   InterPro; IPR020054; Prot_inh_SSI_I16_CS.
DR   InterPro; IPR023549; Subtilisin_inhibitor.
DR   InterPro; IPR036819; Subtilisin_inhibitor-like_sf.
DR   Pfam; PF00720; SSI; 1.
DR   PRINTS; PR00294; SSBTLNINHBTR.
DR   SUPFAM; SSF55399; SSF55399; 1.
DR   PROSITE; PS00999; SSI; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor; Secreted;
KW   Serine protease inhibitor.
FT   CHAIN           1..111
FT                   /note="Subtilisin inhibitor-like protein 8"
FT                   /id="PRO_0000208670"
FT   SITE            71..72
FT                   /note="Reactive bond"
FT   DISULFID        31..46
FT                   /evidence="ECO:0000250"
FT   DISULFID        69..99
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   111 AA;  11436 MW;  02F6F060A04E8530 CRC64;
     SLYAPSAMVF SVAQGDDVAA PTVVRATTVS CAPGARGTHP DPKAACAALK STGGAFDRLL
     SEPNPDRACP MHYAPVTVSA VGVWEGRRVA WDHTFANSCT MAATLDGNAV F