ID   SSI_STRCO               Reviewed;         144 AA.
AC   P61152; P28591; Q9R644;
DT   10-MAY-2004, integrated into UniProtKB/Swiss-Prot.
DT   10-MAY-2004, sequence version 1.
DT   25-MAY-2022, entry version 99.
DE   RecName: Full=Subtilase-type protease inhibitor;
DE   Flags: Precursor;
GN   Name=sti1; OrderedLocusNames=SCO0762; ORFNames=SCF81.21c;
OS   Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces albidoflavus group.
OX   NCBI_TaxID=100226;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-471 / A3(2) / M145;
RX   PubMed=12000953; DOI=10.1038/417141a;
RA   Bentley S.D., Chater K.F., Cerdeno-Tarraga A.-M., Challis G.L.,
RA   Thomson N.R., James K.D., Harris D.E., Quail M.A., Kieser H., Harper D.,
RA   Bateman A., Brown S., Chandra G., Chen C.W., Collins M., Cronin A.,
RA   Fraser A., Goble A., Hidalgo J., Hornsby T., Howarth S., Huang C.-H.,
RA   Kieser T., Larke L., Murphy L.D., Oliver K., O'Neil S., Rabbinowitsch E.,
RA   Rajandream M.A., Rutherford K.M., Rutter S., Seeger K., Saunders D.,
RA   Sharp S., Squares R., Squares S., Taylor K., Warren T., Wietzorrek A.,
RA   Woodward J.R., Barrell B.G., Parkhill J., Hopwood D.A.;
RT   "Complete genome sequence of the model actinomycete Streptomyces coelicolor
RT   A3(2).";
RL   Nature 417:141-147(2002).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 38-144.
RC   STRAIN=A3(2) / NRRL B-16638;
RX   PubMed=8595854; DOI=10.1111/j.1574-6968.1996.tb07984.x;
RA   Taguchi S., Kojima S., Miura K., Momose H.;
RT   "Taxonomic characterization of closely related Streptomyces spp. based on
RT   the amino acid sequence analysis of protease inhibitor proteins.";
RL   FEMS Microbiol. Lett. 135:169-173(1996).
CC   -!- FUNCTION: Strong inhibitory activity toward subtilisin BPN' and, to a
CC       lesser extent, toward trypsin.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- INTERACTION:
CC       P61152; Q9K3X9: SCO1355; NbExp=5; IntAct=EBI-6404783, EBI-6404777;
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I16 (SSI) family.
CC       {ECO:0000305}.
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DR   EMBL; AL939106; CAB61542.1; -; Genomic_DNA.
DR   RefSeq; NP_625065.1; NC_003888.3.
DR   RefSeq; WP_003978099.1; NZ_VNID01000004.1.
DR   AlphaFoldDB; P61152; -.
DR   SMR; P61152; -.
DR   IntAct; P61152; 2.
DR   STRING; 100226.SCO0762; -.
DR   MEROPS; I16.008; -.
DR   PRIDE; P61152; -.
DR   GeneID; 1096185; -.
DR   KEGG; sco:SCO0762; -.
DR   PATRIC; fig|100226.15.peg.753; -.
DR   eggNOG; ENOG50333FU; Bacteria.
DR   HOGENOM; CLU_121949_0_0_11; -.
DR   OMA; TFANECV; -.
DR   Proteomes; UP000001973; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.350.10; -; 1.
DR   HAMAP; MF_00778; SSI; 1.
DR   InterPro; IPR000691; Prot_inh_I16_SSI.
DR   InterPro; IPR020054; Prot_inh_SSI_I16_CS.
DR   InterPro; IPR023549; Subtilisin_inhibitor.
DR   InterPro; IPR036819; Subtilisin_inhibitor-like_sf.
DR   Pfam; PF00720; SSI; 1.
DR   PRINTS; PR00294; SSBTLNINHBTR.
DR   SUPFAM; SSF55399; SSF55399; 1.
DR   PROSITE; PS00999; SSI; 1.
PE   1: Evidence at protein level;
KW   Disulfide bond; Protease inhibitor; Reference proteome; Secreted;
KW   Serine protease inhibitor; Signal.
FT   SIGNAL          1..35
FT                   /evidence="ECO:0000250"
FT   CHAIN           36..144
FT                   /note="Subtilase-type protease inhibitor"
FT                   /id="PRO_0000033272"
FT   SITE            104..105
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000250"
FT   DISULFID        66..81
FT                   /evidence="ECO:0000250"
FT   DISULFID        102..132
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   144 AA;  14433 MW;  E748BADF078A4F8B CRC64;
     MRNTARWAAT LGLTATAVCG PLAGASLASP ATAPASLYAP SALVLTVGHG ESAATAAPLR
     AVTLTCAPTA SGTHPAAAAA CAELRAAHGD PSALAAEDSV MCTREYAPVV VTVDGVWQGR
     RLSYERTFAN ECVKNAGSAS VFTF