ID   SSI_STRGI               Reviewed;         107 AA.
AC   P28592;
DT   01-DEC-1992, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-1992, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Alkaline protease inhibitor 2C';
DE            Short=API-2C';
OS   Streptomyces griseoincarnatus.
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces; Streptomyces griseoincarnatus group.
OX   NCBI_TaxID=29305;
RN   [1]
RP   PROTEIN SEQUENCE.
RA   Suzuki K., Uyeda M., Shibata M.;
RT   "Partial amino acid sequence of an alkaline protease inhibitor, API-2 (b
RT   and c).";
RL   Agric. Biol. Chem. 45:629-634(1981).
RN   [2]
RP   PROTEIN SEQUENCE OF 1-34.
RX   PubMed=7763545; DOI=10.1271/bbb.57.522;
RA   Taguchi S., Kikuchi H., Kojima S., Kumagai I., Nakase T., Miura K.,
RA   Momose H.;
RT   "High frequency of SSI-like protease inhibitors among Streptomyces.";
RL   Biosci. Biotechnol. Biochem. 57:522-524(1993).
CC   -!- FUNCTION: Strong inhibitory activity toward subtilisin.
CC   -!- SUBUNIT: Homodimer.
CC   -!- SUBCELLULAR LOCATION: Secreted.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I16 (SSI) family.
CC       {ECO:0000305}.
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DR   PIR; PC1261; PC1261.
DR   AlphaFoldDB; P28592; -.
DR   SMR; P28592; -.
DR   MEROPS; I16.003; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.350.10; -; 1.
DR   HAMAP; MF_00778; SSI; 1.
DR   InterPro; IPR000691; Prot_inh_I16_SSI.
DR   InterPro; IPR020054; Prot_inh_SSI_I16_CS.
DR   InterPro; IPR023549; Subtilisin_inhibitor.
DR   InterPro; IPR036819; Subtilisin_inhibitor-like_sf.
DR   Pfam; PF00720; SSI; 1.
DR   PRINTS; PR00294; SSBTLNINHBTR.
DR   SUPFAM; SSF55399; SSF55399; 1.
DR   PROSITE; PS00999; SSI; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor; Secreted;
KW   Serine protease inhibitor.
FT   CHAIN           1..107
FT                   /note="Alkaline protease inhibitor 2C'"
FT                   /id="PRO_0000208662"
FT   SITE            67..68
FT                   /note="Reactive bond"
FT   DISULFID        29..44
FT                   /evidence="ECO:0000250"
FT   DISULFID        65..95
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   107 AA;  10972 MW;  76043BA9F876D1CA CRC64;
     YAPSALVLTV GKGVSAATVT PERAVTLTCA PGPSGTHPAA DSACADLAAV GGDLDALTRS
     EGVMCPMIYD PVLLTVDGVW QGERVSYERV FSNECEMNAH GSSVLAF