ID   SSI_STRVL               Reviewed;         146 AA.
AC   O06871;
DT   24-OCT-2001, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1997, sequence version 1.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Subtilisin inhibitor;
DE   Flags: Precursor;
GN   Name=vsi; Synonyms=ssi;
OS   Streptomyces violaceus (Streptomyces venezuelae).
OC   Bacteria; Actinobacteria; Streptomycetales; Streptomycetaceae;
OC   Streptomyces.
OX   NCBI_TaxID=1936;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RC   STRAIN=CBS 762.70;
RX   PubMed=9773272; DOI=10.3109/10425179809050021;
RA   Van Mellaert L., Lammertyn E., Schacht S., Proost P., Van Damme J.,
RA   Wroblowski B., Anne J., Scarcez T., Sablon E., Raeymaeckers J.,
RA   Van Broekhoven A.;
RT   "Molecular characterization of a novel subtilisin inhibitor protein
RT   produced by Streptomyces venezuelae CBS762.70.";
RL   DNA Seq. 9:19-30(1998).
CC   -!- FUNCTION: Strong inhibitor of bacterial serine proteases such as
CC       subtilisin. {ECO:0000305}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the protease inhibitor I16 (SSI) family.
CC       {ECO:0000305}.
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DR   EMBL; X98019; CAA66652.1; -; Genomic_DNA.
DR   AlphaFoldDB; O06871; -.
DR   SMR; O06871; -.
DR   MEROPS; I16.016; -.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   GO; GO:0004867; F:serine-type endopeptidase inhibitor activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.350.10; -; 1.
DR   HAMAP; MF_00778; SSI; 1.
DR   InterPro; IPR000691; Prot_inh_I16_SSI.
DR   InterPro; IPR020054; Prot_inh_SSI_I16_CS.
DR   InterPro; IPR023549; Subtilisin_inhibitor.
DR   InterPro; IPR036819; Subtilisin_inhibitor-like_sf.
DR   Pfam; PF00720; SSI; 1.
DR   PRINTS; PR00294; SSBTLNINHBTR.
DR   SUPFAM; SSF55399; SSF55399; 1.
DR   PROSITE; PS00999; SSI; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Protease inhibitor; Secreted;
KW   Serine protease inhibitor; Signal.
FT   SIGNAL          1..28
FT   CHAIN           29..146
FT                   /note="Subtilisin inhibitor"
FT                   /id="PRO_0000033278"
FT   SITE            104..105
FT                   /note="Reactive bond"
FT                   /evidence="ECO:0000250"
FT   DISULFID        65..80
FT                   /evidence="ECO:0000250"
FT   DISULFID        102..132
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   146 AA;  14629 MW;  A68DD11812A027BA CRC64;
     MRRTLKAVGA AAAAATCVLA ATAGTAQAEA PKAESLYAPS ALVLTVGQGE NAESAAVERA
     VTLTCAPRPG GTHPSAAAAC AELAKVNGQF ARLVGASSDA ICTKEWRPVT VSVVGAWNGK
     HVNWTSTFAN QCTMKAGLGE GAALTF