SSK_DROME
ID SSK_DROME Reviewed; 162 AA.
AC Q9VW87; D5A7R8;
DT 05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 148.
DE RecName: Full=Protein snakeskin {ECO:0000305};
GN Name=Ssk {ECO:0000312|FlyBase:FBgn0036945};
GN ORFNames=CG6981 {ECO:0000312|FlyBase:FBgn0036945};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAK93461.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAK93461.1};
RC TISSUE=Larva {ECO:0000312|EMBL:AAK93461.1}, and
RC Pupae {ECO:0000312|EMBL:AAK93461.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000312|EMBL:ADE60669.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 18-162.
RA Carlson J., Booth B., Frise E., Sandler J., Wan K., Yu C., Celniker S.;
RL Submitted (APR-2010) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP FUNCTION, SUBCELLULAR LOCATION, DEVELOPMENTAL STAGE, AND TOPOLOGY.
RX PubMed=22328496; DOI=10.1242/jcs.096800;
RA Yanagihashi Y., Usui T., Izumi Y., Yonemura S., Sumida M., Tsukita S.,
RA Uemura T., Furuse M.;
RT "Snakeskin, a membrane protein associated with smooth septate junctions, is
RT required for intestinal barrier function in Drosophila.";
RL J. Cell Sci. 125:1980-1990(2012).
RN [6] {ECO:0000305}
RP FUNCTION, INTERACTION WITH MESH, SUBCELLULAR LOCATION, AND DEVELOPMENTAL
RP STAGE.
RX PubMed=22854041; DOI=10.1242/jcs.112243;
RA Izumi Y., Yanagihashi Y., Furuse M.;
RT "A novel protein complex, Mesh-Ssk, is required for septate junction
RT formation in the Drosophila midgut.";
RL J. Cell Sci. 125:4923-4933(2012).
RN [7] {ECO:0000305}
RP FUNCTION, IDENTIFICATION IN A COMPLEX WITH TSP2A AND MESH, SUBCELLULAR
RP LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=26848177; DOI=10.1242/jcs.180448;
RA Izumi Y., Motoishi M., Furuse K., Furuse M.;
RT "A tetraspanin regulates septate junction formation in Drosophila midgut.";
RL J. Cell Sci. 129:1155-1164(2016).
CC -!- FUNCTION: Required for assembly of smooth septate junctions (sSJs),
CC together with mesh and Tsp2A (PubMed:22854041, PubMed:26848177). May be
CC important for barrier function of the midgut epithelium.
CC {ECO:0000269|PubMed:22328496}.
CC -!- SUBUNIT: Forms a complex with Tsp2A and mesh (PubMed:26848177).
CC Interacts with mesh; the interaction may be necessary for the
CC localization of both proteins to the cell apicolateral region
CC (PubMed:22854041). {ECO:0000269|PubMed:22854041,
CC ECO:0000269|PubMed:26848177}.
CC -!- SUBCELLULAR LOCATION: Apicolateral cell membrane
CC {ECO:0000269|PubMed:22328496, ECO:0000269|PubMed:22854041,
CC ECO:0000269|PubMed:26848177}; Multi-pass membrane protein
CC {ECO:0000255}. Cell junction, septate junction
CC {ECO:0000269|PubMed:22328496, ECO:0000269|PubMed:22854041,
CC ECO:0000269|PubMed:26848177}. Note=Specific to smooth septate
CC junctions. {ECO:0000269|PubMed:22328496}.
CC -!- DEVELOPMENTAL STAGE: Detected from embryonic stage 12 onwards in midgut
CC and Malpighian tubules (at protein level) (PubMed:22328496,
CC PubMed:22854041, PubMed:26848177). Also detected in the outer
CC epithelial layer of the proventriculus in first instar larvae (at
CC protein level) (PubMed:22854041). {ECO:0000269|PubMed:22328496,
CC ECO:0000269|PubMed:22854041, ECO:0000269|PubMed:26848177}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ADE60669.1; Type=Miscellaneous discrepancy; Note=Unlikely isoform.; Evidence={ECO:0000305};
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DR EMBL; AE014296; AAF49062.1; -; Genomic_DNA.
DR EMBL; AE014296; AAG22313.1; -; Genomic_DNA.
DR EMBL; AY052037; AAK93461.1; -; mRNA.
DR EMBL; BT122195; ADE60669.1; ALT_SEQ; mRNA.
DR RefSeq; NP_649184.1; NM_140927.5.
DR RefSeq; NP_730487.1; NM_168831.3.
DR AlphaFoldDB; Q9VW87; -.
DR IntAct; Q9VW87; 11.
DR STRING; 7227.FBpp0074589; -.
DR SwissPalm; Q9VW87; -.
DR PaxDb; Q9VW87; -.
DR PRIDE; Q9VW87; -.
DR DNASU; 40207; -.
DR EnsemblMetazoa; FBtr0074820; FBpp0074589; FBgn0036945.
DR EnsemblMetazoa; FBtr0074821; FBpp0074590; FBgn0036945.
DR GeneID; 40207; -.
DR KEGG; dme:Dmel_CG6981; -.
DR UCSC; CG6981-RA; d. melanogaster.
DR CTD; 40207; -.
DR FlyBase; FBgn0036945; Ssk.
DR VEuPathDB; VectorBase:FBgn0036945; -.
DR eggNOG; ENOG502RZZJ; Eukaryota.
DR HOGENOM; CLU_130085_0_0_1; -.
DR InParanoid; Q9VW87; -.
DR OMA; HYWHGYQ; -.
DR OrthoDB; 1368396at2759; -.
DR PhylomeDB; Q9VW87; -.
DR BioGRID-ORCS; 40207; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Ssk; fly.
DR GenomeRNAi; 40207; -.
DR PRO; PR:Q9VW87; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0036945; Expressed in adult midgut (Drosophila) and 27 other tissues.
DR ExpressionAtlas; Q9VW87; baseline and differential.
DR GO; GO:0016327; C:apicolateral plasma membrane; IDA:FlyBase.
DR GO; GO:0005887; C:integral component of plasma membrane; IDA:FlyBase.
DR GO; GO:0005920; C:smooth septate junction; IDA:FlyBase.
DR GO; GO:0007496; P:anterior midgut development; IMP:FlyBase.
DR GO; GO:0060576; P:intestinal epithelial cell development; IMP:FlyBase.
DR GO; GO:0019991; P:septate junction assembly; IMP:FlyBase.
DR InterPro; IPR038976; Ssk.
DR PANTHER; PTHR36692; PTHR36692; 1.
PE 1: Evidence at protein level;
KW Cell junction; Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..162
FT /note="Protein snakeskin"
FT /id="PRO_0000437457"
FT TOPO_DOM 2..6
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:22328496"
FT TRANSMEM 7..27
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 28..53
FT /note="Extracellular"
FT /evidence="ECO:0000305|PubMed:22328496"
FT TRANSMEM 54..74
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 75..88
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305|PubMed:22328496"
FT TRANSMEM 89..109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 110..128
FT /note="Extracellular"
FT /evidence="ECO:0000305|PubMed:22328496"
FT TRANSMEM 129..149
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 150..162
FT /note="Cytoplasmic"
FT /evidence="ECO:0000269|PubMed:22328496"
FT CONFLICT 41
FT /note="T -> A (in Ref. 4; ADE60669)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 162 AA; 17637 MW; D73C59772779FA6F CRC64;
MVSVETVGSI FIKALKLIIN LVIIFLYRWG DGGEFLGIGG TWNLNEEKSA DAEIVASGVM
VGFLIYTGCH TIAFAFGTTK HKGELCDTIM NVVGCIMWIA VGGVALHYWK GYMSDEGFLY
VNSERQVGIA MGSLCVIEGA LYLLDTVLAC IHYSKGDTDY TQ
