SSL10_STAA8
ID SSL10_STAA8 Reviewed; 227 AA.
AC Q2G2X7;
DT 03-JUL-2019, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 1.
DT 25-MAY-2022, entry version 81.
DE RecName: Full=Staphylococcal superantigen-like 10 {ECO:0000303|PubMed:19308288};
DE Flags: Precursor;
GN Name=ssl10 {ECO:0000303|PubMed:19308288}; OrderedLocusNames=SAOUHSC_00395;
OS Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=93061;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC 8325 / PS 47;
RA Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT "The Staphylococcus aureus NCTC 8325 genome.";
RL (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL D.C. (2006).
RN [2]
RP FUNCTION, AND INTERACTION WITH HUMAN CXCR4.
RC STRAIN=NCTC 8325 / PS 47;
RX PubMed=19308288; DOI=10.1593/neo.81508;
RA Walenkamp A.M., Boer I.G., Bestebroer J., Rozeveld D., Timmer-Bosscha H.,
RA Hemrika W., van Strijp J.A., de Haas C.J.;
RT "Staphylococcal superantigen-like 10 inhibits CXCL12-induced human tumor
RT cell migration.";
RL Neoplasia 11:333-344(2009).
RN [3]
RP FUNCTION.
RC STRAIN=ATCC 277933;
RX PubMed=19913916; DOI=10.1016/j.molimm.2009.09.027;
RA Itoh S., Hamada E., Kamoshida G., Yokoyama R., Takii T., Onozaki K.,
RA Tsuji T.;
RT "Staphylococcal superantigen-like protein 10 (SSL10) binds to human
RT immunoglobulin G (IgG) and inhibits complement activation via the classical
RT pathway.";
RL Mol. Immunol. 47:932-938(2010).
RN [4]
RP FUNCTION, AND INTERACTION WITH HOST PROTHROMBIN/F2.
RC STRAIN=ATCC 277933;
RX PubMed=23754290; DOI=10.1074/jbc.m113.451419;
RA Itoh S., Yokoyama R., Kamoshida G., Fujiwara T., Okada H., Takii T.,
RA Tsuji T., Fujii S., Hashizume H., Onozaki K.;
RT "Staphylococcal superantigen-like protein 10 (SSL10) inhibits blood
RT coagulation by binding to prothrombin and factor Xa via their gamma-
RT carboxyglutamic acid (Gla) domain.";
RL J. Biol. Chem. 288:21569-21580(2013).
RN [5]
RP FUNCTION, AND INTERACTION WITH HOST PROTHROMBIN/F2.
RC STRAIN=ATCC 277933;
RX PubMed=28193526; DOI=10.1016/j.bbrc.2017.02.053;
RA Itoh S., Takii T., Onozaki K., Tsuji T., Hida S.;
RT "Identification of the blood coagulation factor interacting sequences in
RT staphylococcal superantigen-like protein 10.";
RL Biochem. Biophys. Res. Commun. 485:201-208(2017).
CC -!- FUNCTION: Plays a role in the inhibition of host complement activation
CC via the classical pathway by interacting with the Fc region of human
CC IgG and thereby interfering with the IgG/C1q interaction
CC (PubMed:19913916). Inhibits also the penultimate step of plasma
CC clotting by interacting with prothrombin/F2 and coagulation factor
CC X/F12. Does not affect the protease activity of thrombin but interferes
CC with the conversion of prothrombin to thrombin (PubMed:23754290,
CC PubMed:28193526). Interacts with human receptor CXCR4 and specifically
CC inhibits CXCL12-induced calcium mobilization and cell migration
CC (PubMed:19308288). {ECO:0000269|PubMed:19308288,
CC ECO:0000269|PubMed:19913916, ECO:0000269|PubMed:23754290,
CC ECO:0000269|PubMed:28193526}.
CC -!- SUBUNIT: Interacts with prothrombin/F2 and coagulation factor X/F12
CC (PubMed:23754290, PubMed:28193526). Interacts with human CXCR4
CC (PubMed:19308288). {ECO:0000269|PubMed:19308288,
CC ECO:0000269|PubMed:23754290, ECO:0000269|PubMed:28193526}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q2G1S8}.
CC -!- SIMILARITY: Belongs to the staphylococcal/streptococcal toxin family.
CC {ECO:0000305}.
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DR EMBL; CP000253; ABD29558.1; -; Genomic_DNA.
DR RefSeq; WP_000673051.1; NZ_LS483365.1.
DR RefSeq; YP_498982.1; NC_007795.1.
DR PDB; 6LWT; X-ray; 1.90 A; A/B=31-227.
DR PDB; 6UCD; X-ray; 2.85 A; A/B=30-227.
DR PDBsum; 6LWT; -.
DR PDBsum; 6UCD; -.
DR AlphaFoldDB; Q2G2X7; -.
DR SMR; Q2G2X7; -.
DR STRING; 1280.SAXN108_0486; -.
DR EnsemblBacteria; ABD29558; ABD29558; SAOUHSC_00395.
DR GeneID; 3919130; -.
DR KEGG; sao:SAOUHSC_00395; -.
DR PATRIC; fig|93061.5.peg.363; -.
DR HOGENOM; CLU_054950_1_0_9; -.
DR OMA; NINALKH; -.
DR Proteomes; UP000008816; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR InterPro; IPR008992; Enterotoxin.
DR InterPro; IPR015282; SSL_OB.
DR InterPro; IPR006126; Staph/Strept_toxin_CS.
DR InterPro; IPR008375; Staph_exotoxin.
DR InterPro; IPR016091; SuperAg_toxin_C.
DR InterPro; IPR013307; Superantigen_bac.
DR InterPro; IPR006123; Toxin_b-grasp_Staph/Strep.
DR Pfam; PF09199; SSL_OB; 1.
DR Pfam; PF02876; Stap_Strp_tox_C; 1.
DR PRINTS; PR01898; SAGSUPRFAMLY.
DR PRINTS; PR01800; STAPHEXOTOXN.
DR PRINTS; PR01501; TOXICSSTOXIN.
DR SUPFAM; SSF50203; SSF50203; 1.
DR SUPFAM; SSF54334; SSF54334; 1.
DR PROSITE; PS00278; STAPH_STREP_TOXIN_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Secreted; Signal; Virulence.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..227
FT /note="Staphylococcal superantigen-like 10"
FT /evidence="ECO:0000255"
FT /id="PRO_5004207952"
FT HELIX 41..49
FT /evidence="ECO:0007829|PDB:6LWT"
FT STRAND 53..64
FT /evidence="ECO:0007829|PDB:6LWT"
FT TURN 65..67
FT /evidence="ECO:0007829|PDB:6LWT"
FT STRAND 68..73
FT /evidence="ECO:0007829|PDB:6LWT"
FT STRAND 76..81
FT /evidence="ECO:0007829|PDB:6LWT"
FT HELIX 84..89
FT /evidence="ECO:0007829|PDB:6LWT"
FT STRAND 92..103
FT /evidence="ECO:0007829|PDB:6LWT"
FT STRAND 105..107
FT /evidence="ECO:0007829|PDB:6LWT"
FT STRAND 112..117
FT /evidence="ECO:0007829|PDB:6LWT"
FT STRAND 119..122
FT /evidence="ECO:0007829|PDB:6LWT"
FT STRAND 133..137
FT /evidence="ECO:0007829|PDB:6LWT"
FT STRAND 140..143
FT /evidence="ECO:0007829|PDB:6UCD"
FT STRAND 147..150
FT /evidence="ECO:0007829|PDB:6LWT"
FT STRAND 155..159
FT /evidence="ECO:0007829|PDB:6LWT"
FT HELIX 160..175
FT /evidence="ECO:0007829|PDB:6LWT"
FT TURN 177..179
FT /evidence="ECO:0007829|PDB:6LWT"
FT STRAND 186..191
FT /evidence="ECO:0007829|PDB:6LWT"
FT STRAND 196..200
FT /evidence="ECO:0007829|PDB:6LWT"
FT HELIX 207..209
FT /evidence="ECO:0007829|PDB:6LWT"
FT STRAND 213..215
FT /evidence="ECO:0007829|PDB:6LWT"
FT HELIX 216..218
FT /evidence="ECO:0007829|PDB:6LWT"
FT STRAND 219..225
FT /evidence="ECO:0007829|PDB:6LWT"
SQ SEQUENCE 227 AA; 26111 MW; 732955609D2333DD CRC64;
MKFTALAKAT LALGILTTGT LTTEVHSGHA KQNQKSVNKH DKEALYRYYT GKTMEMKNIS
ALKHGKNNLR FKFRGIKIQV LLPGNDKSKF QQRSYEGLDV FFVQEKRDKH DIFYTVGGVI
QNNKTSGVVS APILNISKEK GEDAFVKGYP YYIKKEKITL KELDYKLRKH LIEKYGLYKT
ISKDGRVKIS LKDGSFYNLD LRSKLKFKYM GEVIESKQIK DIEVNLK
