ID   SSL3_STAAE              Reviewed;         352 AA.
AC   A0A0H3KEE1;
DT   03-JUL-2019, integrated into UniProtKB/Swiss-Prot.
DT   16-SEP-2015, sequence version 1.
DT   25-MAY-2022, entry version 25.
DE   RecName: Full=Staphylococcal superantigen-like 3 {ECO:0000250|UniProtKB:Q2G0X7};
DE   Flags: Precursor;
GN   Name=ssl3 {ECO:0000250|UniProtKB:Q2G0X7}; OrderedLocusNames=NWMN_0390;
OS   Staphylococcus aureus (strain Newman).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC   Staphylococcus.
OX   NCBI_TaxID=426430;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Newman;
RX   PubMed=17951380; DOI=10.1128/jb.01000-07;
RA   Baba T., Bae T., Schneewind O., Takeuchi F., Hiramatsu K.;
RT   "Genome sequence of Staphylococcus aureus strain Newman and comparative
RT   analysis of staphylococcal genomes: polymorphism and evolution of two major
RT   pathogenicity islands.";
RL   J. Bacteriol. 190:300-310(2008).
CC   -!- FUNCTION: Secreted protein that plays an essential role in immune
CC       innate response inhibition by interacting with and inhibiting host
CC       TLR2. In turn, bacteria recognition by immune cells is impaired and
CC       cytokine production is inhibited. Mechanistically, by interacting with
CC       TLR2, blocks ligand binding and thus inhibits activation. Second, by
CC       interacting with an already formed TLR2-lipopeptide complex, prevents
CC       TLR heterodimerization and downstream signaling. The interaction with
CC       host TLR2 does not involve sialyl Lewis X interactions.
CC       {ECO:0000250|UniProtKB:Q2G0X7}.
CC   -!- SUBUNIT: Interacts with host TLR2 (via its extracellular domain).
CC       {ECO:0000250|UniProtKB:Q2G0X7}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q2G0X7}.
CC   -!- DOMAIN: The C-terminal domain contains a V-shape binding site for
CC       sialyl Lewis X. {ECO:0000250|UniProtKB:Q2G0X7}.
CC   -!- SIMILARITY: Belongs to the staphylococcal/streptococcal toxin family.
CC       {ECO:0000305}.
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DR   EMBL; AP009351; BAF66662.1; -; Genomic_DNA.
DR   RefSeq; WP_000784024.1; NZ_CP023390.1.
DR   AlphaFoldDB; A0A0H3KEE1; -.
DR   SMR; A0A0H3KEE1; -.
DR   EnsemblBacteria; BAF66662; BAF66662; NWMN_0390.
DR   KEGG; sae:NWMN_0390; -.
DR   HOGENOM; CLU_054950_1_0_9; -.
DR   OMA; FMNVIPD; -.
DR   Proteomes; UP000006386; Chromosome.
DR   GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR   InterPro; IPR008992; Enterotoxin.
DR   InterPro; IPR015282; SSL_OB.
DR   InterPro; IPR006126; Staph/Strept_toxin_CS.
DR   InterPro; IPR008375; Staph_exotoxin.
DR   InterPro; IPR016091; SuperAg_toxin_C.
DR   InterPro; IPR013307; Superantigen_bac.
DR   Pfam; PF09199; SSL_OB; 1.
DR   PRINTS; PR01898; SAGSUPRFAMLY.
DR   PRINTS; PR01800; STAPHEXOTOXN.
DR   SUPFAM; SSF50203; SSF50203; 1.
DR   SUPFAM; SSF54334; SSF54334; 1.
DR   PROSITE; PS00278; STAPH_STREP_TOXIN_2; 1.
PE   3: Inferred from homology;
KW   Secreted; Signal; Virulence.
FT   SIGNAL          1..30
FT                   /evidence="ECO:0000255"
FT   CHAIN           31..352
FT                   /note="Staphylococcal superantigen-like 3"
FT                   /evidence="ECO:0000255"
FT                   /id="PRO_5002613612"
FT   REGION          61..165
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          228..326
FT                   /note="Sialyl Lewis X-binding"
FT                   /evidence="ECO:0000250|UniProtKB:Q2G0X7"
FT   COMPBIAS        71..100
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        101..163
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   352 AA;  39739 MW;  87D15E34C8878A4D CRC64;
     MKMRTIAKTS LALGLLTTGA ITVTTQSVKA EKIQSTKVDK VPTLKAERLA MINITAGANS
     ATTQAANTRQ ERTPKLEKAP NTNEEKTSAS KIEKISQPKQ EEQKTLNISA TPAPKQEQSQ
     TTTESTTPKT KVTTPPSTNT PQPMQSTKSD TPQSPTIKQA QTDMTPKYED LRAYYTKPSF
     EFEKQFGFML KPWTTVRFMN VIPNRFIYKI ALVGKDEKKY KDGPYDNIDV FIVLEDNKYQ
     LKKYSVGGIT KTNSKKVNHK VELSITKKDN QGMISRDVSE YMITKEEISL KELDFKLRKQ
     LIEKHNLYGN MGSGTIVIKM KNGGKYTFEL HKKLQEHRMA GTNIDNIEVN IK