SSL4_STAA8
ID SSL4_STAA8 Reviewed; 308 AA.
AC Q2G1S8;
DT 03-JUL-2019, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 1.
DT 25-MAY-2022, entry version 89.
DE RecName: Full=Staphylococcal superantigen-like 4 {ECO:0000303|PubMed:22714643};
DE Flags: Precursor;
GN Name=ssl4 {ECO:0000303|PubMed:22714643}; OrderedLocusNames=SAOUHSC_00389;
OS Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=93061;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC 8325 / PS 47;
RA Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT "The Staphylococcus aureus NCTC 8325 genome.";
RL (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL D.C. (2006).
RN [2]
RP FUNCTION.
RC STRAIN=NCTC 8325 / PS 47;
RX PubMed=22714643; DOI=10.1007/s00109-012-0926-8;
RA Bardoel B.W., Vos R., Bouman T., Aerts P.C., Bestebroer J., Huizinga E.G.,
RA Brondijk T.H., van Strijp J.A., de Haas C.J.;
RT "Evasion of Toll-like receptor 2 activation by staphylococcal superantigen-
RT like protein 3.";
RL J. Mol. Med. 90:1109-1120(2012).
RN [3]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RC STRAIN=NCTC 8325 / PS 47;
RX PubMed=28858870; DOI=10.1159/000479100;
RA Koymans K.J., Goldmann O., Karlsson C.A.Q., Sital W., Thaenert R.,
RA Bisschop A., Vrieling M., Malmstroem J., van Kessel K.P.M., de Haas C.J.C.,
RA van Strijp J.A.G., Medina E.;
RT "The TLR2 Antagonist Staphylococcal Superantigen-Like Protein 3 Acts as a
RT Virulence Factor to Promote Bacterial Pathogenicity in vivo.";
RL J. Innate Immun. 9:561-573(2017).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 116-308.
RG Center for Structural Genomics of Infectious Diseases (CSGID);
RA Filippova E.V., Minasov G., Shuvalova L., Halavaty A., Winsor J.,
RA Dubrovska I., Bagnoli F., Falugi F., Bottomley M., Grandi G.,
RA Anderson W.F.;
RT "Crystal Structure of Superantigen-like Protein, Exotoxin from
RT Staphylococcus aureus subsp. aureus NCTC 8325.";
RL Submitted (NOV-2011) to the PDB data bank.
RN [5]
RP X-RAY CRYSTALLOGRAPHY (1.90 ANGSTROMS) OF 31-308.
RG Center for Structural Genomics of Infectious Diseases (CSGID);
RA Minasov G., Nocadello S., Shuvalova L., Filippova E., Halavaty A.,
RA Dubrovska I., Flores K., Bagnoli F., Falugi F., Bottomley M., Grandi G.,
RA Anderson W.F.;
RT "1.9 Angstrom Crystal Structure of Superantigen-like Protein, Exotoxin from
RT Staphylococcus aureus, in Complex with Sialyl-LewisX.";
RL Submitted (SEP-2014) to the PDB data bank.
CC -!- FUNCTION: Secreted protein that plays a role in immune innate response
CC inhibition by interfering with host TLR2-mediated pathway.
CC {ECO:0000269|PubMed:22714643, ECO:0000269|PubMed:28858870}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:28858870}.
CC -!- DOMAIN: The C-terminal domain contains a V-shape binding site for
CC sialyl Lewis X. {ECO:0000250|UniProtKB:Q2G0X7}.
CC -!- SIMILARITY: Belongs to the staphylococcal/streptococcal toxin family.
CC {ECO:0000305}.
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DR EMBL; CP000253; ABD29552.1; -; Genomic_DNA.
DR RefSeq; WP_000705627.1; NZ_LS483365.1.
DR RefSeq; YP_498975.1; NC_007795.1.
DR PDB; 3URY; X-ray; 1.90 A; A/B=116-308.
DR PDB; 4RCO; X-ray; 1.90 A; A/B=31-308.
DR PDBsum; 3URY; -.
DR PDBsum; 4RCO; -.
DR AlphaFoldDB; Q2G1S8; -.
DR SMR; Q2G1S8; -.
DR STRING; 1280.SAXN108_0480; -.
DR EnsemblBacteria; ABD29552; ABD29552; SAOUHSC_00389.
DR GeneID; 3919124; -.
DR KEGG; sao:SAOUHSC_00389; -.
DR PATRIC; fig|93061.5.peg.357; -.
DR HOGENOM; CLU_054950_1_0_9; -.
DR OMA; NAEQNRT; -.
DR Proteomes; UP000008816; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR InterPro; IPR008992; Enterotoxin.
DR InterPro; IPR015282; SSL_OB.
DR InterPro; IPR006126; Staph/Strept_toxin_CS.
DR InterPro; IPR008375; Staph_exotoxin.
DR InterPro; IPR016091; SuperAg_toxin_C.
DR InterPro; IPR013307; Superantigen_bac.
DR Pfam; PF09199; SSL_OB; 1.
DR PRINTS; PR01898; SAGSUPRFAMLY.
DR PRINTS; PR01800; STAPHEXOTOXN.
DR PRINTS; PR01501; TOXICSSTOXIN.
DR SUPFAM; SSF50203; SSF50203; 1.
DR SUPFAM; SSF54334; SSF54334; 1.
DR PROSITE; PS00278; STAPH_STREP_TOXIN_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Reference proteome; Secreted; Signal; Virulence.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..308
FT /note="Staphylococcal superantigen-like 4"
FT /evidence="ECO:0000255"
FT /id="PRO_5004207852"
FT REGION 32..117
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 180..278
FT /note="Sialyl Lewis X-binding"
FT /evidence="ECO:0000250|UniProtKB:Q2G0X7"
FT COMPBIAS 32..115
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT HELIX 118..120
FT /evidence="ECO:0007829|PDB:3URY"
FT HELIX 121..126
FT /evidence="ECO:0007829|PDB:3URY"
FT STRAND 132..141
FT /evidence="ECO:0007829|PDB:3URY"
FT STRAND 146..153
FT /evidence="ECO:0007829|PDB:3URY"
FT STRAND 155..158
FT /evidence="ECO:0007829|PDB:3URY"
FT STRAND 160..164
FT /evidence="ECO:0007829|PDB:3URY"
FT HELIX 168..171
FT /evidence="ECO:0007829|PDB:3URY"
FT STRAND 174..184
FT /evidence="ECO:0007829|PDB:3URY"
FT STRAND 194..198
FT /evidence="ECO:0007829|PDB:3URY"
FT STRAND 201..203
FT /evidence="ECO:0007829|PDB:3URY"
FT STRAND 211..220
FT /evidence="ECO:0007829|PDB:3URY"
FT STRAND 226..235
FT /evidence="ECO:0007829|PDB:3URY"
FT STRAND 238..241
FT /evidence="ECO:0007829|PDB:3URY"
FT HELIX 242..257
FT /evidence="ECO:0007829|PDB:3URY"
FT TURN 259..261
FT /evidence="ECO:0007829|PDB:3URY"
FT STRAND 266..272
FT /evidence="ECO:0007829|PDB:3URY"
FT STRAND 277..281
FT /evidence="ECO:0007829|PDB:3URY"
FT HELIX 288..291
FT /evidence="ECO:0007829|PDB:3URY"
FT STRAND 294..296
FT /evidence="ECO:0007829|PDB:3URY"
FT HELIX 297..299
FT /evidence="ECO:0007829|PDB:3URY"
FT STRAND 300..307
FT /evidence="ECO:0007829|PDB:3URY"
SQ SEQUENCE 308 AA; 34072 MW; C66F6A69B8D6BF25 CRC64;
MKITTIAKTS LALGLLTTGV ITTTTQAANA TTLSSTKVEA PQSTPPSTKI EAPQSKPNAT
TPPSTKVEAP QQTANATTPP STKVTTPPST NTPQPMQSTK SDTPQSPTTK QVPTEINPKF
KDLRAYYTKP SLEFKNEIGI ILKKWTTIRF MNVVPDYFIY KIALVGKDDK KYGEGVHRNV
DVFVVLEENN YNLEKYSVGG ITKSNSKKVD HKAGVRITKE DNKGTISHDV SEFKITKEQI
SLKELDFKLR KQLIEKNNLY GNVGSGKIVI KMKNGGKYTF ELHKKLQENR MADVIDGTNI
DNIEVNIK
