SSL5_STAA8
ID SSL5_STAA8 Reviewed; 234 AA.
AC Q2G1S6;
DT 05-JUN-2019, integrated into UniProtKB/Swiss-Prot.
DT 21-MAR-2006, sequence version 1.
DT 25-MAY-2022, entry version 84.
DE RecName: Full=Staphylococcal superantigen-like 5 {ECO:0000303|PubMed:17132726};
DE Flags: Precursor;
GN Name=ssl5 {ECO:0000303|PubMed:17132726}; OrderedLocusNames=SAOUHSC_00390;
OS Staphylococcus aureus (strain NCTC 8325 / PS 47).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=93061;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NCTC 8325 / PS 47;
RA Gillaspy A.F., Worrell V., Orvis J., Roe B.A., Dyer D.W., Iandolo J.J.;
RT "The Staphylococcus aureus NCTC 8325 genome.";
RL (In) Fischetti V., Novick R., Ferretti J., Portnoy D., Rood J. (eds.);
RL Gram positive pathogens, 2nd edition, pp.381-412, ASM Press, Washington
RL D.C. (2006).
RN [2]
RP FUNCTION, AND INTERACTION WITH HOST SELPLG.
RC STRAIN=NCTC 8325 / PS 47;
RX PubMed=17132726; DOI=10.1182/blood-2006-06-015461;
RA Bestebroer J., Poppelier M.J., Ulfman L.H., Lenting P.J., Denis C.V.,
RA van Kessel K.P., van Strijp J.A., de Haas C.J.;
RT "Staphylococcal superantigen-like 5 binds PSGL-1 and inhibits P-selectin-
RT mediated neutrophil rolling.";
RL Blood 109:2936-2943(2007).
RN [3]
RP FUNCTION, AND INTERACTION WITH HOST MMP9.
RC STRAIN=ATCC 27733;
RX PubMed=20479083; DOI=10.1128/iai.00178-10;
RA Itoh S., Hamada E., Kamoshida G., Takeshita K., Oku T., Tsuji T.;
RT "Staphylococcal superantigen-like protein 5 inhibits matrix
RT metalloproteinase 9 from human neutrophils.";
RL Infect. Immun. 78:3298-3305(2010).
RN [4]
RP FUNCTION, INTERACTION WITH HOST GP1BA AND GP6, AND MUTAGENESIS OF THR-205.
RC STRAIN=NCTC 8325 / PS 47;
RX PubMed=21552524; DOI=10.1371/journal.pone.0019190;
RA Hu H., Armstrong P.C., Khalil E., Chen Y.C., Straub A., Li M.,
RA Soosairajah J., Hagemeyer C.E., Bassler N., Huang D., Ahrens I.,
RA Krippner G., Gardiner E., Peter K.;
RT "GPVI and GPIbalpha mediate staphylococcal superantigen-like protein 5
RT (SSL5) induced platelet activation and direct toward glycans as potential
RT inhibitors.";
RL PLoS ONE 6:E19190-E19190(2011).
RN [5]
RP FUNCTION, AND INTERACTION WITH HOST MMP9.
RX PubMed=29462623; DOI=10.1016/j.bbrc.2018.02.138;
RA Kohno K., Itoh S., Hanai A., Takii T., Fujiwara T., Onozaki K., Tsuji T.,
RA Hida S.;
RT "Identification of matrix metalloproteinase 9-interacting sequences in
RT staphylococcal superantigen-like protein 5.";
RL Biochem. Biophys. Res. Commun. 497:713-718(2018).
RN [6]
RP INTERACTION WITH HOST MMP9.
RX PubMed=29328525; DOI=10.1111/1348-0421.12573;
RA Kurisaka C., Oku T., Itoh S., Tsuji T.;
RT "Role of sialic acid-containing glycans of matrix metalloproteinase-9 (MMP-
RT 9) in the interaction between MMP-9 and staphylococcal superantigen-like
RT protein 5.";
RL Microbiol. Immunol. 62:168-175(2018).
CC -!- FUNCTION: Secreted protein that plays a role in the inhibition of host
CC innate immune system. Modulates the interaction between host SELPLG and
CC P-selectin thereby preventing initial rolling of neutrophils toward the
CC site of infection (PubMed:17132726). Interferes with leukocyte
CC trafficking by inhibiting host metalloproteinase-9/MMP9 activity
CC (PubMed:20479083, PubMed:29462623, PubMed:29328525). Associates also
CC with two different platelet surface receptors GP1A and GP6 leading to
CC platelet activation and aggregation (PubMed:21552524).
CC {ECO:0000269|PubMed:17132726, ECO:0000269|PubMed:20479083,
CC ECO:0000269|PubMed:21552524, ECO:0000269|PubMed:29328525,
CC ECO:0000269|PubMed:29462623}.
CC -!- SUBUNIT: Interacts with host SELPLG; this interaction prevents SELPLG-
CC mediated neutrophil rolling (PubMed:17132726). Interacts with host MMP9
CC (via sialic acid-containing O-glycans); this interaction inhibits MMP9
CC activity. Interacts with host GP1BA and GP6; these interactions play an
CC important role in platelet binding and activation (PubMed:21552524).
CC {ECO:0000269|PubMed:17132726, ECO:0000269|PubMed:21552524}.
CC -!- SIMILARITY: Belongs to the staphylococcal/streptococcal toxin family.
CC {ECO:0000305}.
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DR EMBL; CP000253; ABD29553.1; -; Genomic_DNA.
DR RefSeq; WP_000784244.1; NZ_LS483365.1.
DR RefSeq; YP_498976.1; NC_007795.1.
DR AlphaFoldDB; Q2G1S6; -.
DR SMR; Q2G1S6; -.
DR STRING; 1280.SAXN108_0481; -.
DR EnsemblBacteria; ABD29553; ABD29553; SAOUHSC_00390.
DR GeneID; 3919125; -.
DR KEGG; sao:SAOUHSC_00390; -.
DR PATRIC; fig|93061.5.peg.358; -.
DR eggNOG; ENOG503054K; Bacteria.
DR HOGENOM; CLU_054950_1_0_9; -.
DR OMA; YIYKEEV; -.
DR Proteomes; UP000008816; Chromosome.
DR GO; GO:0005576; C:extracellular region; IEA:InterPro.
DR InterPro; IPR008992; Enterotoxin.
DR InterPro; IPR015282; SSL_OB.
DR InterPro; IPR006126; Staph/Strept_toxin_CS.
DR InterPro; IPR008375; Staph_exotoxin.
DR InterPro; IPR016091; SuperAg_toxin_C.
DR InterPro; IPR013307; Superantigen_bac.
DR Pfam; PF09199; SSL_OB; 1.
DR PRINTS; PR01898; SAGSUPRFAMLY.
DR PRINTS; PR01800; STAPHEXOTOXN.
DR PRINTS; PR01501; TOXICSSTOXIN.
DR SUPFAM; SSF50203; SSF50203; 1.
DR SUPFAM; SSF54334; SSF54334; 1.
DR PROSITE; PS00278; STAPH_STREP_TOXIN_2; 1.
PE 1: Evidence at protein level;
KW Reference proteome; Signal.
FT SIGNAL 1..30
FT /evidence="ECO:0000255"
FT CHAIN 31..234
FT /note="Staphylococcal superantigen-like 5"
FT /evidence="ECO:0000255"
FT /id="PRO_5004207838"
FT MUTAGEN 205
FT /note="T->P: Complete loss of interaction with GP1BA."
FT /evidence="ECO:0000269|PubMed:21552524"
SQ SEQUENCE 234 AA; 27170 MW; B8D2BA9C7FEC2CE9 CRC64;
MKMTAIAKAS LALGILATGT ITSLHQTVNA SEHKAKYENV TKDIFDLRDY YSGASKELKN
VTGYRYSKGG KHYLIFDKNR KFTRVQIFGK DIERFKARKN PGLDIFVVKE AENRNGTVFS
YGGVTKKNQD AYYDYINAPR FQIKRDEGDG IATYGRVHYI YKEEISLKEL DFKLRQYLIQ
NFDLYKKFPK DSKIKVIMKD GGYYTFELNK KLQTNRMSDV IDGRNIEKIE ANIR
