ID   SSN2_CANGA              Reviewed;        1345 AA.
AC   Q6FNM7;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   19-JUL-2004, sequence version 1.
DT   25-MAY-2022, entry version 98.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 13;
DE   AltName: Full=Mediator complex subunit 13;
GN   Name=SSN2; Synonyms=MED13; OrderedLocusNames=CAGL0J10472g;
OS   Candida glabrata (strain ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL
OS   Y-65) (Yeast) (Torulopsis glabrata).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Nakaseomyces;
OC   Nakaseomyces/Candida clade.
OX   NCBI_TaxID=284593;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 2001 / CBS 138 / JCM 3761 / NBRC 0622 / NRRL Y-65;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Component of the SRB8-11 complex. The SRB8-11 complex is a
CC       regulatory module of the Mediator complex which is itself involved in
CC       regulation of basal and activated RNA polymerase II-dependent
CC       transcription. The SRB8-11 complex may be involved in the
CC       transcriptional repression of a subset of genes regulated by Mediator.
CC       It may inhibit the association of the Mediator complex with RNA
CC       polymerase II to form the holoenzyme complex (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Component of the SRB8-11 complex, which itself associates with
CC       the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 13 family.
CC       {ECO:0000305}.
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DR   EMBL; CR380956; CAG61118.1; -; Genomic_DNA.
DR   RefSeq; XP_448167.1; XM_448167.1.
DR   AlphaFoldDB; Q6FNM7; -.
DR   SMR; Q6FNM7; -.
DR   STRING; 5478.XP_448167.1; -.
DR   PRIDE; Q6FNM7; -.
DR   EnsemblFungi; CAG61118; CAG61118; CAGL0J10472g.
DR   GeneID; 2889605; -.
DR   KEGG; cgr:CAGL0J10472g; -.
DR   CGD; CAL0132930; CAGL0J10472g.
DR   VEuPathDB; FungiDB:CAGL0J10472g; -.
DR   eggNOG; KOG3600; Eukaryota.
DR   HOGENOM; CLU_242296_0_0_1; -.
DR   InParanoid; Q6FNM7; -.
DR   OMA; FSRELWC; -.
DR   Proteomes; UP000002428; Chromosome J.
DR   GO; GO:1990508; C:CKM complex; IEA:EnsemblFungi.
DR   GO; GO:0016592; C:mediator complex; IEA:EnsemblFungi.
DR   GO; GO:0003713; F:transcription coactivator activity; IEA:EnsemblFungi.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:EnsemblFungi.
DR   GO; GO:0000435; P:positive regulation of transcription from RNA polymerase II promoter by galactose; IEA:EnsemblFungi.
DR   GO; GO:0006468; P:protein phosphorylation; IEA:EnsemblFungi.
DR   InterPro; IPR009401; Med13_C.
DR   InterPro; IPR021643; Mediator_Med13_N.
DR   Pfam; PF06333; Med13_C; 1.
DR   Pfam; PF11597; Med13_N; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1345
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   13"
FT                   /id="PRO_0000314247"
FT   REGION          363..387
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          402..537
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        363..381
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        402..417
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        444..467
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        468..484
FT                   /note="Acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        485..514
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1345 AA;  151209 MW;  E99C8B215112B0AE CRC64;
     MKEATDDYDL SKLVSNFYRL EKITKINYAQ YLPKKQNDQW GIQMEIQMRK KDRKLLVALL
     SKELWCFSIN DQVLPSPNDI DPELNVSADK TGSFTADYSK PNLPPHYALF LKSLKRMIYL
     NLVEQSKNTL VQFGNSCINI TKNNESNNLL QIEPHLFSNS ELAISICVKD LGLLPLKVNS
     IDPAFLSSHA LYMAPSGVRV YLVENKNDNS GTNQEHLHKY LVPVPENGEV LLKTLYASHG
     IKLNSSTCQW VNIIPNITHL NGRAPNISQY MDLPKELRTI VWPLDLVFAQ PALDIDSSTT
     LSQINGLDTF EDTLSLIDNF LQLKQSSSYR TPGSSGGLTG TNIGTNVFSS EGAYTDQFQV
     YPKSQTNQQQ TSSNSKVSPS DAASPYPGID KIIEQKQFTP DFMASPSVSG NSNELFNDRK
     NGHTDLLISP SKMDVDTDFP GSNLAGQEAP SQPASDVKKS SVSASDSELF GEEDEDEDDA
     DLFGESNNDS TGESNANNSK GEITEDIFAS SDDESSLQPK KESTFYDGIK NGSAESGLNM
     QDRANLKRTY LDIRIEETPL SSPLYTDPGA PLPVETPRDR RKSVFAPLNF NPIIAKDVDN
     KYKNGGKYSF SPLQKEEALN FDVSRTELSS SEGEDSESSD DELEDLANKN DGVVYDKAEN
     IGYKTFANLQ DSVPPGLIKQ DFLGNPYLAS LEGSKEGQNT IWKMPQTDIA QTESPLKAID
     TSIGPDGTLP NTTSSEQSKS VYVHDYGISP TKMSNDPAFD TAKIEGKYEF MNNFPSSFPF
     LLRHMPLSLI PDTFKHLNPT ITVNERNNQI IDLLAEQIVY DYNILGNLTI PDVPYSGIRN
     YSNGVVKNTI DNLFSEFTRL DGTTLISRLY PMETPFVSVR KQHDQIKLRS DVQQFTKYAN
     LKPVRGIKNF KFLVLTDSFK EDCIQFISSL SQTYINHELG FCEHLQLTNE DSKGLIYLKD
     FEDSKLLLLA AQIVSYLSTN RTSGKEVAFM MIIPVQRCDI SELVEKTAKF QIIQNEVKAK
     IPSMELYLKI VPMDFIKSPL TSVDDYTNLC ISIYNILPNK MIKFTHIRKQ IPEKLTFKTS
     QQASAFKYDA YIHLAYSRSV DKQWMFAALS DSAGKENMMK TWYLGSSKNK FDEACNHIWE
     MALSLAGKNY GKVCLILTRL NGILPDDELM NWRRLSGRNI HLAVVCVDDN TKISFFDRNE
     SYPSYKRLYQ NANSIETLVD PERIDDYLIR DLDQDIHGVI FENPFPLVNS LHRCAIKSGA
     LVKFNVSTTA TEPHSLDKFE VNLLNCPHSD SFKLLETILE EFRNLAALNV WFGITNGENG
     HIPWHVLAVK KMMKTLVHTR VKVAQ