ID   SSN2_KLULA              Reviewed;        1387 AA.
AC   Q6CWU8;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   16-AUG-2004, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 13;
DE   AltName: Full=Mediator complex subunit 13;
GN   Name=SSN2; Synonyms=MED13; OrderedLocusNames=KLLA0B01353g;
OS   Kluyveromyces lactis (strain ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 /
OS   NRRL Y-1140 / WM37) (Yeast) (Candida sphaerica).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX   NCBI_TaxID=284590;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 8585 / CBS 2359 / DSM 70799 / NBRC 1267 / NRRL Y-1140 / WM37;
RX   PubMed=15229592; DOI=10.1038/nature02579;
RA   Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I.,
RA   de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L.,
RA   Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S.,
RA   Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J.,
RA   Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E.,
RA   Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C.,
RA   Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M.,
RA   Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S.,
RA   Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F.,
RA   Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M.,
RA   Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M.,
RA   Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C.,
RA   Weissenbach J., Wincker P., Souciet J.-L.;
RT   "Genome evolution in yeasts.";
RL   Nature 430:35-44(2004).
CC   -!- FUNCTION: Component of the SRB8-11 complex. The SRB8-11 complex is a
CC       regulatory module of the Mediator complex which is itself involved in
CC       regulation of basal and activated RNA polymerase II-dependent
CC       transcription. The SRB8-11 complex may be involved in the
CC       transcriptional repression of a subset of genes regulated by Mediator.
CC       It may inhibit the association of the Mediator complex with RNA
CC       polymerase II to form the holoenzyme complex (By similarity).
CC       {ECO:0000250}.
CC   -!- SUBUNIT: Component of the SRB8-11 complex, which itself associates with
CC       the Mediator complex. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 13 family.
CC       {ECO:0000305}.
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DR   EMBL; CR382122; CAH01984.1; -; Genomic_DNA.
DR   RefSeq; XP_451591.1; XM_451591.1.
DR   AlphaFoldDB; Q6CWU8; -.
DR   SMR; Q6CWU8; -.
DR   STRING; 28985.XP_451591.1; -.
DR   PRIDE; Q6CWU8; -.
DR   EnsemblFungi; CAH01984; CAH01984; KLLA0_B01353g.
DR   GeneID; 2896865; -.
DR   KEGG; kla:KLLA0_B01353g; -.
DR   eggNOG; KOG3600; Eukaryota.
DR   HOGENOM; CLU_242296_0_0_1; -.
DR   InParanoid; Q6CWU8; -.
DR   OMA; FSRELWC; -.
DR   Proteomes; UP000000598; Chromosome B.
DR   GO; GO:1990508; C:CKM complex; IEA:EnsemblFungi.
DR   GO; GO:0016592; C:mediator complex; IEA:EnsemblFungi.
DR   GO; GO:0003713; F:transcription coactivator activity; IEA:EnsemblFungi.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IEA:EnsemblFungi.
DR   GO; GO:0000435; P:positive regulation of transcription from RNA polymerase II promoter by galactose; IEA:EnsemblFungi.
DR   GO; GO:0006468; P:protein phosphorylation; IEA:EnsemblFungi.
DR   InterPro; IPR009401; Med13_C.
DR   InterPro; IPR021643; Mediator_Med13_N.
DR   Pfam; PF06333; Med13_C; 2.
DR   Pfam; PF11597; Med13_N; 1.
PE   3: Inferred from homology;
KW   Activator; Nucleus; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1387
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   13"
FT                   /id="PRO_0000314249"
FT   REGION          81..102
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          354..400
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          620..676
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        620..662
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   1387 AA;  155993 MW;  F9A151FB2D295076 CRC64;
     MKKEVGDACR LEDIVTNLYR LETVKQINYH QYVPSKADNQ WSIQAELFLR RKNPKVLAAL
     FSRELWCFSI NDDPLPELNL ELNNESESPQ PERKGHFTPE FSKPHLPTPY AIFMKALRRM
     IYVNLTLSSN ETIVPFGNSC IFQESYTFST KILHFDPHLF ENGDLTVAIC SKDLGLSKLN
     AESLKHEVAV YLAPSGIRVY LPSTDLKKCY VSPPKNAQMF LKTLFVSHGI NLIDVDGLKW
     VKLIPNANHL NGFTPTISHY LDEPKGNNFV IWPACLCFVQ TASDVQTPQY TAFSRSPQLE
     LDDCFDMIDG FIQLKLTSAY RTPGTSAGMG TVTGHNPLST GGIFTDQFQG FNKHSANNSN
     NVSSTGENGK FSPEYSNDPN VTPLRDNKTS RQNFPTESYS SNGFITTPII NENITPTVDD
     IITETPSVKP QNDLWNEKKD VINSTSNINS KDASVGSSER KVELETAFES PAQNISIEEH
     EHVEFDKDLF GEDSDEDVST RNKNEELVSV KEITDEMFDL AEDDDEADGS TSNSLKFDRP
     DTIESIDTDE KKTRTKRTYL DIPVDVITIE KTPTLYEDPG APLPIETPKD RKKSIFAPLN
     FNPIFESNVD NKYKNGGKFS VNSNTNDEPI QFGISTSNIS SSEDDDSDFS PADFNNNGPS
     AGKGLSYDPR DNDIPMIESS TYEPIAKDSL PELINPPSSS KDDSVASYNT IGTLMEKPVK
     SNLDAIWKPA LSKSERADLP NQTINGVIND CNNSSNSNPT TYFENTPSLG PDTLYDNSKP
     RSSSANFLST VEIDPKSEQT IQTAEQEQSE SSRILPYLLR HMPLFSLPDV FLCQNPSLPP
     GKDFEDILEI LTDQIVFNND MFSDDRATES QFKGIKDCSV GTISDTMNQL FGNFSKLHGN
     EIIEEVFYLP EPSVFVKKSE DTIKIKSSSC YFTEYLNLKP NRGVKNFRAL VLTTEAKNDC
     MSFVSQMSQT YSNHELGFCE LTKLTNDDNK GLIYLKNFNQ DTLLLLSAQI VSFCSTALSN
     IKNIPLLIFL PINKLSLTEC ISMILKFHVI RKEFKSKLPK ADILLRLVNI DFLKNPLTPI
     TAYTSLCMAI YNSLPPKSTK VTSLTNDLPK QIEFRTLKNA SLSIHYDNYI HLAYLRSIDR
     EWLCAAWSDT KGVESFVKTW YVGNSRTRFE QVCNDIWKIT LQLASKNFGN VCLVLTRLDS
     VLPDDELIHW RRLSVATKDL HLAVVCVGDN TKLTLFDEDK TYPTFKNLFR TKSNPQTGIS
     NSIDDLEIIN IDEEVHGLIF SNPLQLANSQ HRCAIKSGAL IRFAKSEGDN FIDKFEVNLL
     NCPHADSSTL LKEILKQYRN LAGLNPWFGV SYGKDNFIPW HVVAVKNVMN SIVHVKSSFE
     KETHIID