ID   SSN2_SCHPO              Reviewed;        1225 AA.
AC   Q9HE02;
DT   13-APR-2004, integrated into UniProtKB/Swiss-Prot.
DT   22-SEP-2009, sequence version 2.
DT   03-AUG-2022, entry version 107.
DE   RecName: Full=Mediator of RNA polymerase II transcription subunit 13;
DE   AltName: Full=Mediator complex subunit 13;
DE   AltName: Full=Suppressor of RNA polymerase B srb9;
GN   Name=srb9; Synonyms=med13, trap240; ORFNames=SPAC589.02c;
OS   Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Taphrinomycotina;
OC   Schizosaccharomycetes; Schizosaccharomycetales; Schizosaccharomycetaceae;
OC   Schizosaccharomyces.
OX   NCBI_TaxID=284812;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=972 / ATCC 24843;
RX   PubMed=11859360; DOI=10.1038/nature724;
RA   Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A.,
RA   Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S.,
RA   Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.,
RA   Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S.,
RA   Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S.,
RA   Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D.,
RA   Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P.,
RA   Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K.,
RA   O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M.,
RA   Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N.,
RA   Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A.,
RA   Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R.,
RA   Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M.,
RA   Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A.,
RA   Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A.,
RA   Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H.,
RA   Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S.,
RA   Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C.,
RA   Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A.,
RA   Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M.,
RA   del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S.,
RA   Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R.,
RA   Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G.,
RA   Nurse P.;
RT   "The genome sequence of Schizosaccharomyces pombe.";
RL   Nature 415:871-880(2002).
RN   [2]
RP   IDENTIFICATION IN MEDIATOR COMPLEX.
RX   PubMed=12738880; DOI=10.1073/pnas.1030497100;
RA   Samuelsen C.O., Baraznenok V., Khorosjutina O., Spaehr H., Kieselbach T.,
RA   Holmberg S., Gustafsson C.M.;
RT   "TRAP230/ARC240 and TRAP240/ARC250 Mediator subunits are functionally
RT   conserved through evolution.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:6422-6427(2003).
RN   [3]
RP   FUNCTION.
RX   PubMed=14534314; DOI=10.1074/jbc.m306750200;
RA   Spaehr H., Khorosjutina O., Baraznenok V., Linder T., Samuelsen C.O.,
RA   Hermand D., Maekelae T.P., Holmberg S., Gustafsson C.M.;
RT   "Mediator influences Schizosaccharomyces pombe RNA polymerase II-dependent
RT   transcription in vitro.";
RL   J. Biol. Chem. 278:51301-51306(2003).
RN   [4]
RP   SUBCELLULAR LOCATION.
RX   PubMed=16630887; DOI=10.1016/j.molcel.2006.03.032;
RA   Zhu X., Wiren M., Sinha I., Rasmussen N.N., Linder T., Holmberg S.,
RA   Ekwall K., Gustafsson C.M.;
RT   "Genome-wide occupancy profile of mediator and the Srb8-11 module reveals
RT   interactions with coding regions.";
RL   Mol. Cell 22:169-178(2006).
CC   -!- FUNCTION: Component of the srb8-11 complex. The srb8-11 complex is a
CC       regulatory module of the Mediator complex which is itself involved in
CC       regulation of basal and activated RNA polymerase II-dependent
CC       transcription. The srb8-11 complex may be involved in the
CC       transcriptional repression of a subset of genes regulated by Mediator.
CC       It may inhibit the association of the Mediator complex with RNA
CC       polymerase II to form the holoenzyme complex.
CC       {ECO:0000269|PubMed:14534314}.
CC   -!- SUBUNIT: Component of the srb8-11 complex which consists of rb8,
CC       srb9(TRAP240), srb10 and srb11. The srb8-11 complex associates with the
CC       Mediator complex thereby blocking association with RNA polymerase II
CC       and leading to reduced transcriptional activation by Mediator.
CC       {ECO:0000269|PubMed:12738880}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:16630887}.
CC   -!- SIMILARITY: Belongs to the Mediator complex subunit 13 family.
CC       {ECO:0000305}.
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DR   EMBL; CU329670; CAC19759.2; -; Genomic_DNA.
DR   RefSeq; NP_594050.2; NM_001019474.2.
DR   AlphaFoldDB; Q9HE02; -.
DR   BioGRID; 279027; 59.
DR   IntAct; Q9HE02; 1.
DR   STRING; 4896.SPAC589.02c.1; -.
DR   iPTMnet; Q9HE02; -.
DR   MaxQB; Q9HE02; -.
DR   PaxDb; Q9HE02; -.
DR   PRIDE; Q9HE02; -.
DR   EnsemblFungi; SPAC589.02c.1; SPAC589.02c.1:pep; SPAC589.02c.
DR   GeneID; 2542571; -.
DR   KEGG; spo:SPAC589.02c; -.
DR   PomBase; SPAC589.02c; -.
DR   VEuPathDB; FungiDB:SPAC589.02c; -.
DR   HOGENOM; CLU_279001_0_0_1; -.
DR   InParanoid; Q9HE02; -.
DR   OMA; GGKYWCP; -.
DR   PRO; PR:Q9HE02; -.
DR   Proteomes; UP000002485; Chromosome I.
DR   GO; GO:0005829; C:cytosol; HDA:PomBase.
DR   GO; GO:0016592; C:mediator complex; IPI:PomBase.
DR   GO; GO:0005634; C:nucleus; HDA:PomBase.
DR   GO; GO:0003713; F:transcription coactivator activity; IC:PomBase.
DR   GO; GO:0003712; F:transcription coregulator activity; IBA:GO_Central.
DR   GO; GO:0022408; P:negative regulation of cell-cell adhesion; IMP:PomBase.
DR   GO; GO:0060261; P:positive regulation of transcription initiation from RNA polymerase II promoter; IC:PomBase.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   InterPro; IPR009401; Med13_C.
DR   Pfam; PF06333; Med13_C; 1.
PE   1: Evidence at protein level;
KW   Activator; Nucleus; Reference proteome; Repressor; Transcription;
KW   Transcription regulation.
FT   CHAIN           1..1225
FT                   /note="Mediator of RNA polymerase II transcription subunit
FT                   13"
FT                   /id="PRO_0000072184"
SQ   SEQUENCE   1225 AA;  139261 MW;  19C84096CEEB99BD CRC64;
     MSYQVCFKKY VKMRQWPHVL SLRVYASYFL LYNNLPLSSA MVHDGVHLIS IKTILTDQPC
     PNTIYYAKYQ VQRKDNEDAA SLLEDKEAYL RNQDCIVHAK NDLLFVYDFQ AIPSIPEESS
     SFMLLNSGAF SRLALFQKDE LALLLDLYIN FLQGLKKTVL YWLCKEYNFV PIYGVLLPLK
     LHPSFDTQLW NIYGYPVVDL QLSVLSKGHI EFYLKPTRQT VYRLSEVDNL VKKLDTVIRL
     APTGCLATLT SVHANASAQT VDALKHRYGF SLTTTSKWVG VTLESSALEF SWPLELCFLE
     TSALRMNDDS LSSNLTDLNN LVLPSNVVNN KKELTEFANE EAEASDKRKE GFTEKEETAD
     AVVTLVPSHS SSPVNYSINS AKSTPASIKV NEEILVADHN VSDDILMEEI DDVGITEADF
     DYFDLPNVEE KVEMIEPNFA NTMTTLDNEE INTSISQSNT SPNLNTHENI PKQMEIQSDD
     RMVTEDLNPY NVEVDIPEIS LNISDSKIPT SAYMPSYYSA VIFPSSISSI FQKYNYGGKY
     WCPSPSLSTE DLFESFSVAE SVTSTDEDIC STNFIQQDFT MEYNHDFFSS SKTPTNISEQ
     SNPDSNYDTL SLAHQVLMNE SKSANFDFSF LKSLDLQPTI TLGKNDLLNA ILSQNLWFRS
     LPFWKSMTTS FMMSQDVLNF SSYMRKPIRD YLEKILLGES SAVFLSKSPE NYLSSINNGH
     HALNDNPPSQ VNFSETLVNF SQPPRVLLKY NEKKLSLDSS APENWISLCL QPYGESKDFE
     VFLLSSKSPD VSSKAISFFY DVQLAYENCK LGKLNLSETS INERVMGFST NINETDNYDD
     NETTQSDTAT SYEQLASVCV NELSGKNVLF FYFLEDDSEK LLKACQHFIC VKDSIKRLGD
     NKFEDKSLRI CTIPNSIFDS PNSHTTNSNS FFTKVSLDIY NNDPLLMDGS LKRREPAFLL
     KKPLLSTLNY QLKDINPRSS ALGEYALHVT YTTVEEHLLI CNWNDSYGEF ETERRYFLQD
     LEIEDALQQI LEVTFTFLNS MHMDWIVIVM KIGEMSDAEY LFWDQAIIPE NLQGNVSLTV
     GYCSAEHGPG STSKVFSRIP YSASSTVIRN NSSHELSLVA FIREMAMPVP NDEFKKISTI
     LARGYLALDE DESYLPLLSI HLLISRNHDP YLMLNLILKH YLSMIYLQFR TYVSFSSLPL
     HISTVLYQKQ LLQFMASDIT HPVTS