SSNA1_MOUSE
ID SSNA1_MOUSE Reviewed; 119 AA.
AC Q9JJ94;
DT 13-SEP-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 138.
DE RecName: Full=Microtubule nucleation factor SSNA1 {ECO:0000303|PubMed:30250060};
DE AltName: Full=Sjoegren syndrome nuclear autoantigen 1 homolog;
GN Name=Ssna1 {ECO:0000312|MGI:MGI:1915725}; ORFNames=MNCb-2711;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Brain;
RA Osada N., Kusuda J., Tanuma R., Ito A., Hirata M., Sugano S., Hashimoto K.;
RT "Isolation of full-length cDNA clones from mouse brain cDNA library made by
RT oligo-capping method.";
RL Submitted (APR-2000) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=NOD; TISSUE=Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Czech II, and FVB/N; TISSUE=Liver, and Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=30250060; DOI=10.1038/s41556-018-0199-8;
RA Basnet N., Nedozralova H., Crevenna A.H., Bodakuntla S., Schlichthaerle T.,
RA Taschner M., Cardone G., Janke C., Jungmann R., Magiera M.M.,
RA Biertuempfel C., Mizuno N.;
RT "Direct induction of microtubule branching by microtubule nucleation factor
RT SSNA1.";
RL Nat. Cell Biol. 20:1172-1180(2018).
CC -!- FUNCTION: Microtubule-binding protein which stabilizes dynamic
CC microtubules by slowing growth and shrinkage at both plus and minus
CC ends and serves as a sensor of microtubule damage, protecting
CC microtubules from the microtubule-severing enzyme SPAST (By
CC similarity). Induces microtubule branching which is mediated by the
CC formation of long SSNA1 fibrils which guide microtubule protofilaments
CC to split apart from the mother microtubule and form daughter
CC microtubules (By similarity). Plays a role in axon outgrowth and
CC branching (PubMed:30250060). Required for cell division (By
CC similarity). {ECO:0000250|UniProtKB:O43805,
CC ECO:0000250|UniProtKB:Q9XF62, ECO:0000269|PubMed:30250060}.
CC -!- SUBUNIT: Self-associates to form fibrils. Also forms dimers as well as
CC monomers. Interacts with SPAST. {ECO:0000250|UniProtKB:O43805}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000250|UniProtKB:O43805}.
CC Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
CC {ECO:0000250|UniProtKB:O43805}. Cytoplasm, cytoskeleton, microtubule
CC organizing center, centrosome, centriole
CC {ECO:0000250|UniProtKB:O43805}. Midbody {ECO:0000250|UniProtKB:O43805}.
CC Cytoplasm, cytoskeleton, flagellum basal body
CC {ECO:0000250|UniProtKB:O43805}. Cytoplasm, cytoskeleton, flagellum
CC axoneme {ECO:0000250|UniProtKB:O43805}. Cell projection, axon
CC {ECO:0000269|PubMed:30250060}. Note=In sperm, strongly expressed in the
CC basal body region with weaker expression in the axoneme (By
CC similarity). Localizes to axon branching points in neurons
CC (PubMed:30250060). {ECO:0000250|UniProtKB:O43805,
CC ECO:0000269|PubMed:30250060}.
CC -!- SIMILARITY: Belongs to the SSNA1 family. {ECO:0000305}.
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DR EMBL; AB041656; BAA95103.1; -; mRNA.
DR EMBL; AK088418; BAC40344.1; -; mRNA.
DR EMBL; BC037081; AAH37081.1; -; mRNA.
DR EMBL; BC048360; AAH48360.1; -; mRNA.
DR CCDS; CCDS38071.1; -.
DR RefSeq; NP_075953.1; NM_023464.2.
DR AlphaFoldDB; Q9JJ94; -.
DR SMR; Q9JJ94; -.
DR BioGRID; 212871; 3.
DR IntAct; Q9JJ94; 4.
DR STRING; 10090.ENSMUSP00000028342; -.
DR PhosphoSitePlus; Q9JJ94; -.
DR EPD; Q9JJ94; -.
DR MaxQB; Q9JJ94; -.
DR PaxDb; Q9JJ94; -.
DR PeptideAtlas; Q9JJ94; -.
DR PRIDE; Q9JJ94; -.
DR ProteomicsDB; 262697; -.
DR Antibodypedia; 32398; 107 antibodies from 23 providers.
DR DNASU; 68475; -.
DR Ensembl; ENSMUST00000028342; ENSMUSP00000028342; ENSMUSG00000026966.
DR GeneID; 68475; -.
DR KEGG; mmu:68475; -.
DR UCSC; uc008irc.1; mouse.
DR CTD; 8636; -.
DR MGI; MGI:1915725; Ssna1.
DR VEuPathDB; HostDB:ENSMUSG00000026966; -.
DR eggNOG; ENOG502S16M; Eukaryota.
DR GeneTree; ENSGT00390000012318; -.
DR HOGENOM; CLU_153440_0_0_1; -.
DR InParanoid; Q9JJ94; -.
DR OMA; SYNNELV; -.
DR OrthoDB; 1513306at2759; -.
DR PhylomeDB; Q9JJ94; -.
DR TreeFam; TF328451; -.
DR Reactome; R-MMU-2565942; Regulation of PLK1 Activity at G2/M Transition.
DR Reactome; R-MMU-380259; Loss of Nlp from mitotic centrosomes.
DR Reactome; R-MMU-380270; Recruitment of mitotic centrosome proteins and complexes.
DR Reactome; R-MMU-380284; Loss of proteins required for interphase microtubule organization from the centrosome.
DR Reactome; R-MMU-380320; Recruitment of NuMA to mitotic centrosomes.
DR Reactome; R-MMU-5620912; Anchoring of the basal body to the plasma membrane.
DR Reactome; R-MMU-8854518; AURKA Activation by TPX2.
DR BioGRID-ORCS; 68475; 0 hits in 71 CRISPR screens.
DR ChiTaRS; Ssna1; mouse.
DR PRO; PR:Q9JJ94; -.
DR Proteomes; UP000000589; Chromosome 2.
DR RNAct; Q9JJ94; protein.
DR Bgee; ENSMUSG00000026966; Expressed in spermatocyte and 267 other tissues.
DR Genevisible; Q9JJ94; MM.
DR GO; GO:0030424; C:axon; IDA:UniProtKB.
DR GO; GO:0005930; C:axoneme; ISS:UniProtKB.
DR GO; GO:0005814; C:centriole; ISS:UniProtKB.
DR GO; GO:0005813; C:centrosome; IDA:MGI.
DR GO; GO:0036064; C:ciliary basal body; IDA:MGI.
DR GO; GO:0030496; C:midbody; ISS:UniProtKB.
DR GO; GO:0005634; C:nucleus; IEA:UniProtKB-SubCell.
DR GO; GO:0099512; C:supramolecular fiber; ISS:UniProtKB.
DR GO; GO:0042802; F:identical protein binding; IPI:MGI.
DR GO; GO:0008017; F:microtubule binding; ISO:MGI.
DR GO; GO:0043621; F:protein self-association; ISS:UniProtKB.
DR GO; GO:0140060; P:axon arborization; ISS:UniProtKB.
DR GO; GO:0048675; P:axon extension; IMP:UniProtKB.
DR GO; GO:0007409; P:axonogenesis; IMP:UniProtKB.
DR GO; GO:0051301; P:cell division; ISS:UniProtKB.
DR GO; GO:0060830; P:ciliary receptor clustering involved in smoothened signaling pathway; IMP:MGI.
DR GO; GO:0042073; P:intraciliary transport; IMP:MGI.
DR GO; GO:0000226; P:microtubule cytoskeleton organization; ISS:UniProtKB.
DR GO; GO:0007020; P:microtubule nucleation; ISO:MGI.
DR InterPro; IPR033362; SSNA1_fam.
DR PANTHER; PTHR28661; PTHR28661; 1.
PE 1: Evidence at protein level;
KW Acetylation; Cell cycle; Cell division; Cell projection; Cilium;
KW Coiled coil; Cytoplasm; Cytoskeleton; Flagellum; Neurogenesis; Nucleus;
KW Reference proteome.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250|UniProtKB:O43805"
FT CHAIN 2..119
FT /note="Microtubule nucleation factor SSNA1"
FT /id="PRO_0000114484"
FT REGION 2..32
FT /note="Important for localization to the centrosome"
FT /evidence="ECO:0000250|UniProtKB:O43805"
FT COILED 13..70
FT /evidence="ECO:0000255"
FT MOD_RES 2
FT /note="N-acetylthreonine"
FT /evidence="ECO:0000250|UniProtKB:O43805"
SQ SEQUENCE 119 AA; 13557 MW; DFACFB6942CDA2DD CRC64;
MTQQGAALQN YNNELVKCIE ELCQKREELC RQIQQEEDEK QRLQNEVRQL TEKLARVNEN
LARKIASRNE FDRTIAETEA AYLKILESSQ TLLSVLKREA GNLTKATASD QKSSGGKDS
