SSO2_YEAST
ID SSO2_YEAST Reviewed; 295 AA.
AC P39926; D6W007;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1996, sequence version 2.
DT 03-AUG-2022, entry version 179.
DE RecName: Full=Protein SSO2;
GN Name=SSO2; OrderedLocusNames=YMR183C; ORFNames=YM8010.13C;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=ATCC 26787 / X2180-1B;
RX PubMed=8223426; DOI=10.1002/j.1460-2075.1993.tb06093.x;
RA Aalto M.K., Ronne H., Keraenen S.;
RT "Yeast syntaxins Sso1p and Sso2p belong to a family of related membrane
RT proteins that function in vesicular transport.";
RL EMBO J. 12:4095-4104(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169872;
RA Bowman S., Churcher C.M., Badcock K., Brown D., Chillingworth T.,
RA Connor R., Dedman K., Devlin K., Gentles S., Hamlin N., Hunt S., Jagels K.,
RA Lye G., Moule S., Odell C., Pearson D., Rajandream M.A., Rice P.,
RA Skelton J., Walsh S.V., Whitehead S., Barrell B.G.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XIII.";
RL Nature 387:90-93(1997).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-31 AND SER-34, AND
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=18407956; DOI=10.1074/mcp.m700468-mcp200;
RA Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.;
RT "A multidimensional chromatography technology for in-depth phosphoproteome
RT analysis.";
RL Mol. Cell. Proteomics 7:1389-1396(2008).
CC -!- FUNCTION: Required for vesicle fusion with the plasma membrane.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type IV
CC membrane protein {ECO:0000305}.
CC -!- MISCELLANEOUS: Present with 1720 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the syntaxin family. {ECO:0000305}.
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DR EMBL; X67730; CAA47960.1; -; mRNA.
DR EMBL; Z49808; CAA89916.1; -; Genomic_DNA.
DR EMBL; BK006946; DAA10081.1; -; Genomic_DNA.
DR PIR; S55130; S55130.
DR RefSeq; NP_013908.1; NM_001182689.1.
DR PDB; 5LG4; X-ray; 2.90 A; A=36-227.
DR PDB; 5M4Y; X-ray; 2.20 A; A/C/E=36-227.
DR PDBsum; 5LG4; -.
DR PDBsum; 5M4Y; -.
DR AlphaFoldDB; P39926; -.
DR SMR; P39926; -.
DR BioGRID; 35361; 387.
DR ComplexPortal; CPX-1369; Vesicular SNARE complex SSO2-SEC9-SNC1.
DR ComplexPortal; CPX-5465; Vesicular SNARE complex SSO2-SEC9-SNC2.
DR DIP; DIP-2951N; -.
DR IntAct; P39926; 5.
DR MINT; P39926; -.
DR STRING; 4932.YMR183C; -.
DR CarbonylDB; P39926; -.
DR iPTMnet; P39926; -.
DR SwissPalm; P39926; -.
DR MaxQB; P39926; -.
DR PaxDb; P39926; -.
DR PRIDE; P39926; -.
DR EnsemblFungi; YMR183C_mRNA; YMR183C; YMR183C.
DR GeneID; 855221; -.
DR KEGG; sce:YMR183C; -.
DR SGD; S000004795; SSO2.
DR VEuPathDB; FungiDB:YMR183C; -.
DR eggNOG; KOG0810; Eukaryota.
DR GeneTree; ENSGT01000000214440; -.
DR HOGENOM; CLU_042423_0_1_1; -.
DR InParanoid; P39926; -.
DR OMA; RWICFIL; -.
DR BioCyc; YEAST:G3O-32871-MON; -.
DR Reactome; R-SCE-114516; Disinhibition of SNARE formation.
DR Reactome; R-SCE-199992; trans-Golgi Network Vesicle Budding.
DR Reactome; R-SCE-9609523; Insertion of tail-anchored proteins into the endoplasmic reticulum membrane.
DR PRO; PR:P39926; -.
DR Proteomes; UP000002311; Chromosome XIII.
DR RNAct; P39926; protein.
DR GO; GO:0071944; C:cell periphery; HDA:SGD.
DR GO; GO:0005737; C:cytoplasm; HDA:SGD.
DR GO; GO:0012505; C:endomembrane system; IBA:GO_Central.
DR GO; GO:0005783; C:endoplasmic reticulum; HDA:SGD.
DR GO; GO:0000139; C:Golgi membrane; IC:ComplexPortal.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IDA:ComplexPortal.
DR GO; GO:0005886; C:plasma membrane; IDA:SGD.
DR GO; GO:0005628; C:prospore membrane; IDA:SGD.
DR GO; GO:0031201; C:SNARE complex; IPI:ComplexPortal.
DR GO; GO:0070300; F:phosphatidic acid binding; IDA:SGD.
DR GO; GO:0005484; F:SNAP receptor activity; IPI:SGD.
DR GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR GO; GO:0031321; P:ascospore-type prospore assembly; IGI:SGD.
DR GO; GO:0006887; P:exocytosis; IBA:GO_Central.
DR GO; GO:0006893; P:Golgi to plasma membrane transport; TAS:SGD.
DR GO; GO:0048210; P:Golgi vesicle fusion to target membrane; IC:ComplexPortal.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0048278; P:vesicle docking; IBA:GO_Central.
DR GO; GO:0006906; P:vesicle fusion; IDA:ComplexPortal.
DR GO; GO:0099500; P:vesicle fusion to plasma membrane; IC:ComplexPortal.
DR CDD; cd00179; SynN; 1.
DR DisProt; DP01504; -.
DR InterPro; IPR010989; SNARE.
DR InterPro; IPR045242; Syntaxin.
DR InterPro; IPR006012; Syntaxin/epimorphin_CS.
DR InterPro; IPR006011; Syntaxin_N.
DR InterPro; IPR000727; T_SNARE_dom.
DR PANTHER; PTHR19957; PTHR19957; 1.
DR Pfam; PF05739; SNARE; 1.
DR Pfam; PF00804; Syntaxin; 1.
DR SMART; SM00503; SynN; 1.
DR SMART; SM00397; t_SNARE; 1.
DR SUPFAM; SSF47661; SSF47661; 1.
DR PROSITE; PS00914; SYNTAXIN; 1.
DR PROSITE; PS50192; T_SNARE; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Coiled coil; Membrane; Phosphoprotein; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..295
FT /note="Protein SSO2"
FT /id="PRO_0000210275"
FT TOPO_DOM 1..269
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 270..291
FT /note="Helical; Anchor for type IV membrane protein"
FT /evidence="ECO:0000255"
FT TOPO_DOM 292..295
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT DOMAIN 194..256
FT /note="t-SNARE coiled-coil homology"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00202"
FT COILED 39..100
FT /evidence="ECO:0000255"
FT MOD_RES 31
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT MOD_RES 34
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:18407956"
FT CONFLICT 222
FT /note="E -> K (in Ref. 1; CAA47960)"
FT /evidence="ECO:0000305"
FT HELIX 36..68
FT /evidence="ECO:0007829|PDB:5M4Y"
FT HELIX 74..108
FT /evidence="ECO:0007829|PDB:5M4Y"
FT TURN 109..111
FT /evidence="ECO:0007829|PDB:5LG4"
FT HELIX 113..155
FT /evidence="ECO:0007829|PDB:5M4Y"
FT STRAND 157..159
FT /evidence="ECO:0007829|PDB:5LG4"
FT HELIX 161..168
FT /evidence="ECO:0007829|PDB:5M4Y"
FT STRAND 169..172
FT /evidence="ECO:0007829|PDB:5M4Y"
FT TURN 174..177
FT /evidence="ECO:0007829|PDB:5LG4"
FT HELIX 192..222
FT /evidence="ECO:0007829|PDB:5M4Y"
SQ SEQUENCE 295 AA; 33734 MW; 454CF2B45FFE8E22 CRC64;
MSNANPYENN NPYAENYEMQ EDLNNAPTGH SDGSDDFVAF MNKINSINAN LSRYENIINQ
IDAQHKDLLT QVSEEQEMEL RRSLDDYISQ ATDLQYQLKA DIKDAQRDGL HDSNKQAQAE
NCRQKFLKLI QDYRIIDSNY KEESKEQAKR QYTIIQPEAT DEEVEAAIND VNGQQIFSQA
LLNANRRGEA KTALAEVQAR HQELLKLEKT MAELTQLFND MEELVIEQQE NVDVIDKNVE
DAQQDVEQGV GHTNKAVKSA RKARKNKIRC LIICFIIFAI VVVVVVVPSV VETRK
