SSPA_DICDI
ID SSPA_DICDI Reviewed; 406 AA.
AC Q55A00; Q86IM6; Q86IM7;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 25-MAY-2022, entry version 81.
DE RecName: Full=Probable sphingosine-1-phosphate phosphatase;
DE Short=SPPase;
DE EC=3.1.3.-;
GN Name=sppA; ORFNames=DDB_G0272260;
OS Dictyostelium discoideum (Slime mold).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=12097910; DOI=10.1038/nature00847;
RA Gloeckner G., Eichinger L., Szafranski K., Pachebat J.A., Bankier A.T.,
RA Dear P.H., Lehmann R., Baumgart C., Parra G., Abril J.F., Guigo R.,
RA Kumpf K., Tunggal B., Cox E.C., Quail M.A., Platzer M., Rosenthal A.,
RA Noegel A.A.;
RT "Sequence and analysis of chromosome 2 of Dictyostelium discoideum.";
RL Nature 418:79-85(2002).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Has enzymatic activity against both sphingosine 1 phosphate
CC (S1P) and dihydro-S1P. Regulates intracellular and extracellular S1P
CC levels (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC Multi-pass membrane protein {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the type 2 lipid phosphate phosphatase family.
CC {ECO:0000305}.
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DR EMBL; AAFI02000008; EAL71282.1; -; Genomic_DNA.
DR RefSeq; XP_645184.1; XM_640092.1.
DR AlphaFoldDB; Q55A00; -.
DR STRING; 44689.DDB0216187; -.
DR PaxDb; Q55A00; -.
DR EnsemblProtists; EAL71282; EAL71282; DDB_G0272260.
DR GeneID; 8618356; -.
DR KEGG; ddi:DDB_G0272260; -.
DR dictyBase; DDB_G0272260; sppA.
DR eggNOG; KOG2822; Eukaryota.
DR HOGENOM; CLU_678676_0_0_1; -.
DR InParanoid; Q55A00; -.
DR OMA; NINEYEC; -.
DR PhylomeDB; Q55A00; -.
DR Reactome; R-DDI-1660661; Sphingolipid de novo biosynthesis.
DR PRO; PR:Q55A00; -.
DR Proteomes; UP000002195; Chromosome 2.
DR GO; GO:0005783; C:endoplasmic reticulum; ISS:dictyBase.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0016020; C:membrane; IBA:GO_Central.
DR GO; GO:0042392; F:sphingosine-1-phosphate phosphatase activity; ISS:dictyBase.
DR GO; GO:0046839; P:phospholipid dephosphorylation; IBA:GO_Central.
DR InterPro; IPR036938; P_Acid_Pase_2/haloperoxi_sf.
DR InterPro; IPR000326; P_Acid_Pase_2/haloperoxidase.
DR Pfam; PF01569; PAP2; 1.
DR SMART; SM00014; acidPPc; 1.
DR SUPFAM; SSF48317; SSF48317; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum; Hydrolase; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..406
FT /note="Probable sphingosine-1-phosphate phosphatase"
FT /id="PRO_0000365604"
FT TRANSMEM 66..86
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 92..112
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 138..158
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 162..182
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 193..213
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 225..245
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 254..274
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 313..333
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 374..394
FT /note="Helical"
FT /evidence="ECO:0000255"
FT REGION 107..115
FT /note="Phosphatase sequence motif I"
FT /evidence="ECO:0000305"
FT REGION 133..136
FT /note="Phosphatase sequence motif II"
FT /evidence="ECO:0000305"
FT REGION 183..194
FT /note="Phosphatase sequence motif III"
FT /evidence="ECO:0000305"
FT ACT_SITE 136
FT /note="Proton donor"
FT /evidence="ECO:0000250|UniProtKB:P0A924"
FT ACT_SITE 190
FT /note="Nucleophile"
FT /evidence="ECO:0000250|UniProtKB:P0A924"
FT SITE 194
FT /note="Stabilizes the active site histidine for
FT nucleophilic attack"
FT /evidence="ECO:0000250|UniProtKB:P0A924"
SQ SEQUENCE 406 AA; 45887 MW; E2CEF8E700CA0049 CRC64;
MEKIKKQNNI GSYSSDKDGE YKKFRFSKTS IRGFLLKEYE REVPIIVKIQ SYRNKFLDFY
FKMASILGEE VFFILALPIS TWCVATQLGV ELCVVLALTI GGGNILKNTF TLPRPPPNIV
WTNTAHQKDH GLPSTHTASA FGLTFYFLIY TYFLFPTIGE SFNISLLSMF FIVLFWSTSV
MFSRLYNGHH TPMDVIAGLI VAITSILATT YQLRYFIEGM VLSETFLFGP MLYIAILSAI
LFFHPQANTG PTPAYPETGL VCGASLGSLI SLWLHAQHPC PLMNQELLLL EANYDSIVST
IHSIPLLLHG SRILIGLVLV GIAKVFSKKF FFFAYDLVIR ANTNNEQSQP ITTVSFDPNK
KIIVTPTIEA FSKLFVYTCV SFTIVSMPYL FYYLNIQTSA DVTRYY