SSPN_MOUSE
ID SSPN_MOUSE Reviewed; 216 AA.
AC Q62147; Q60594;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Sarcospan;
DE AltName: Full=K-ras oncogene-associated protein;
DE AltName: Full=Kirsten-Ras-associated protein;
GN Name=Sspn; Synonyms=Krag;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
RC STRAIN=LAF;
RX PubMed=8020969; DOI=10.1006/geno.1994.1157;
RA Scott A.F., Elizaga A., Morrell J., Bergen A., Penno M.B.;
RT "Characterization of a gene coamplified with Ki-ras in Y1 murine adrenal
RT carcinoma cells that codes for a putative membrane protein.";
RL Genomics 20:227-230(1994).
RN [2]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Component of the dystrophin-glycoprotein complex (DGC), a
CC complex that spans the muscle plasma membrane and forms a link between
CC the F-actin cytoskeleton and the extracellular matrix. Preferentially
CC associates with the sarcoglycan subcomplex of the DGC (By similarity).
CC {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}. Cell membrane, sarcolemma {ECO:0000250}.
CC Postsynaptic cell membrane {ECO:0000250}; Multi-pass membrane protein
CC {ECO:0000250}. Note=Also found in myotendinous junctions and in the
CC postsynaptic membrane of neuromuscular junctions. {ECO:0000250}.
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DR EMBL; U02486; AAB60682.1; -; Genomic_DNA.
DR EMBL; U02484; AAB60682.1; JOINED; Genomic_DNA.
DR EMBL; U02485; AAB60682.1; JOINED; Genomic_DNA.
DR EMBL; U02487; AAA03712.1; -; mRNA.
DR CCDS; CCDS20698.1; -.
DR PIR; A54305; A54305.
DR RefSeq; NP_034786.1; NM_010656.2.
DR AlphaFoldDB; Q62147; -.
DR BioGRID; 201011; 1.
DR CORUM; Q62147; -.
DR STRING; 10090.ENSMUSP00000032383; -.
DR iPTMnet; Q62147; -.
DR PhosphoSitePlus; Q62147; -.
DR PaxDb; Q62147; -.
DR PRIDE; Q62147; -.
DR ProteomicsDB; 258743; -.
DR DNASU; 16651; -.
DR GeneID; 16651; -.
DR KEGG; mmu:16651; -.
DR CTD; 8082; -.
DR MGI; MGI:1353511; Sspn.
DR eggNOG; ENOG502RYPH; Eukaryota.
DR InParanoid; Q62147; -.
DR OrthoDB; 1462432at2759; -.
DR PhylomeDB; Q62147; -.
DR BioGRID-ORCS; 16651; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Sspn; mouse.
DR PRO; PR:Q62147; -.
DR Proteomes; UP000000589; Unplaced.
DR RNAct; Q62147; protein.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0016010; C:dystrophin-associated glycoprotein complex; IEA:InterPro.
DR GO; GO:0005887; C:integral component of plasma membrane; IEA:InterPro.
DR GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR GO; GO:0045211; C:postsynaptic membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0042383; C:sarcolemma; IDA:MGI.
DR GO; GO:0030133; C:transport vesicle; ISO:MGI.
DR InterPro; IPR007237; CD20-like.
DR InterPro; IPR030429; Sarcospan.
DR PANTHER; PTHR15260; PTHR15260; 1.
DR Pfam; PF04103; CD20; 1.
PE 1: Evidence at protein level;
KW Cell membrane; Membrane; Postsynaptic cell membrane; Reference proteome;
KW Synapse; Transmembrane; Transmembrane helix.
FT CHAIN 1..216
FT /note="Sarcospan"
FT /id="PRO_0000072227"
FT TOPO_DOM 1..26
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 27..47
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 48..59
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 81..95
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 96..116
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 117..166
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 167..187
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 188..216
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 65
FT /note="S -> I (in Ref. 1; AAA03712)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 216 AA; 23859 MW; 19033582EFA9D2AD CRC64;
MGRKPSPRAQ ELPEEEARTC CGCRFPLLLA LLQLALGIAV TVLGFLMASI SPSLLVRDTP
FWAGSIVCVV AYLGLFMLCV SYQVDERTCV QFSMKVFYFL LSALGLMVCM LAVAFAAHHY
SLLAQFTCET SLDSCQCKLP SSEPLSRAFV YRDVTDCTSV TGTFKLFLII QMVLNLVCGL
VCLLACFVMW KHRYQVFYVG VGLRSLMASD GQLPKA
