ID   SSPO_BACSU              Reviewed;          48 AA.
AC   P71031;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 3.
DT   03-AUG-2022, entry version 114.
DE   RecName: Full=Small, acid-soluble spore protein O;
DE            Short=SASP O;
GN   Name=sspO; Synonyms=cotK; OrderedLocusNames=BSU17990;
OS   Bacillus subtilis (strain 168).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX   NCBI_TaxID=224308;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9068633; DOI=10.1128/jb.179.6.1887-1897.1997;
RA   Henriques A.O., Beall B.W., Moran C.P. Jr.;
RT   "CotM of Bacillus subtilis, a member of the alpha-crystallin family of
RT   stress proteins, is induced during development and participates in spore
RT   outer coat formation.";
RL   J. Bacteriol. 179:1887-1897(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=168;
RX   PubMed=9384377; DOI=10.1038/36786;
RA   Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA   Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA   Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA   Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA   Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA   Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA   Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA   Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA   Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA   Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA   Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA   Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA   Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA   Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA   Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA   Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA   Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA   Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA   Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA   Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA   Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA   Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA   Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA   Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA   Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA   Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA   Yoshikawa H., Danchin A.;
RT   "The complete genome sequence of the Gram-positive bacterium Bacillus
RT   subtilis.";
RL   Nature 390:249-256(1997).
RN   [3]
RP   PROTEIN SEQUENCE OF 2-13.
RX   PubMed=9852018; DOI=10.1128/jb.180.24.6704-6712.1998;
RA   Bagyan I., Setlow B., Setlow P.;
RT   "New small, acid-soluble proteins unique to spores of Bacillus subtilis:
RT   identification of the coding genes and regulation and function of two of
RT   these genes.";
RL   J. Bacteriol. 180:6704-6712(1998).
RN   [4]
RP   SUBCELLULAR LOCATION, AND INDUCTION.
RX   PubMed=10806362; DOI=10.1016/s0378-1119(00)00125-6;
RA   Cabrera-Hernandez A., Setlow P.;
RT   "Analysis of the regulation and function of five genes encoding small,
RT   acid-soluble spore proteins of Bacillus subtilis.";
RL   Gene 248:169-181(2000).
CC   -!- SUBCELLULAR LOCATION: Spore core {ECO:0000269|PubMed:10806362}.
CC   -!- INDUCTION: Expressed only in the forespore compartment of sporulating
CC       cells. Expression is sigma G-dependent. {ECO:0000269|PubMed:10806362}.
CC   -!- SIMILARITY: Belongs to the SspO family. {ECO:0000305}.
CC   -!- CAUTION: Was originally thought to be a spore coat protein.
CC       {ECO:0000305|PubMed:9068633}.
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DR   EMBL; U72073; AAC44987.1; -; Genomic_DNA.
DR   EMBL; AL009126; CAB13683.1; -; Genomic_DNA.
DR   PIR; A69606; A69606.
DR   RefSeq; NP_389682.1; NC_000964.3.
DR   RefSeq; WP_003244839.1; NZ_JNCM01000035.1.
DR   AlphaFoldDB; P71031; -.
DR   STRING; 224308.BSU17990; -.
DR   PaxDb; P71031; -.
DR   EnsemblBacteria; CAB13683; CAB13683; BSU_17990.
DR   GeneID; 938222; -.
DR   KEGG; bsu:BSU17990; -.
DR   PATRIC; fig|224308.179.peg.1960; -.
DR   BioCyc; BSUB:BSU17990-MON; -.
DR   Proteomes; UP000001570; Chromosome.
DR   GO; GO:0042601; C:endospore-forming forespore; IEA:InterPro.
DR   GO; GO:0030436; P:asexual sporulation; IEA:UniProtKB-UniRule.
DR   GO; GO:0030435; P:sporulation resulting in formation of a cellular spore; IEA:UniProtKB-KW.
DR   HAMAP; MF_00665; SspO; 1.
DR   InterPro; IPR012613; SASP_SspO.
DR   TIGRFAMs; TIGR02864; spore_sspO; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Reference proteome; Sporulation.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000269|PubMed:9852018"
FT   CHAIN           2..48
FT                   /note="Small, acid-soluble spore protein O"
FT                   /id="PRO_0000217208"
FT   REGION          1..22
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   48 AA;  5427 MW;  54770583F475994B CRC64;
     MVKRKANHVI NGMNDAKSQG KGAGYIENDQ LVLTEAERQN NKKRKTNQ