ID   SSRA_DICDI              Reviewed;         236 AA.
AC   Q54R45;
DT   29-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-MAY-2005, sequence version 1.
DT   25-MAY-2022, entry version 80.
DE   RecName: Full=Translocon-associated protein subunit alpha;
DE            Short=TRAP-alpha;
DE   AltName: Full=Signal sequence receptor subunit alpha;
DE            Short=SSR-alpha;
DE   Flags: Precursor;
GN   Name=ssr1; ORFNames=DDB_G0283497;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
CC   -!- FUNCTION: TRAP proteins are part of a complex whose function is to bind
CC       calcium to the ER membrane and thereby regulate the retention of ER
CC       resident proteins. {ECO:0000250}.
CC   -!- SUBUNIT: Heterotrimer of TRAP-alpha, TRAP-beta and TRAP-gamma.
CC       {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass type I membrane protein {ECO:0000250}.
CC   -!- DOMAIN: Shows a remarkable charge distribution with the N-terminus
CC       being highly negatively charged, and the cytoplasmic C-terminus
CC       positively charged. {ECO:0000250}.
CC   -!- PTM: Phosphorylated in its cytoplasmic tail. {ECO:0000250}.
CC   -!- MISCELLANEOUS: Seems to bind calcium. {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TRAP-alpha family. {ECO:0000305}.
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DR   EMBL; AAFI02000055; EAL65733.1; -; Genomic_DNA.
DR   RefSeq; XP_639046.1; XM_633954.1.
DR   AlphaFoldDB; Q54R45; -.
DR   STRING; 44689.DDB0266492; -.
DR   PaxDb; Q54R45; -.
DR   EnsemblProtists; EAL65733; EAL65733; DDB_G0283497.
DR   GeneID; 8624071; -.
DR   KEGG; ddi:DDB_G0283497; -.
DR   dictyBase; DDB_G0283497; ssr1.
DR   eggNOG; KOG1631; Eukaryota.
DR   HOGENOM; CLU_073618_2_0_1; -.
DR   InParanoid; Q54R45; -.
DR   OMA; FNFHIQN; -.
DR   PhylomeDB; Q54R45; -.
DR   PRO; PR:Q54R45; -.
DR   Proteomes; UP000002195; Chromosome 4.
DR   GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0045335; C:phagocytic vesicle; HDA:dictyBase.
DR   InterPro; IPR005595; TRAP_alpha.
DR   Pfam; PF03896; TRAP_alpha; 1.
PE   3: Inferred from homology;
KW   Calcium; Endoplasmic reticulum; Glycoprotein; Membrane; Phosphoprotein;
KW   Reference proteome; Signal; Transmembrane; Transmembrane helix.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..236
FT                   /note="Translocon-associated protein subunit alpha"
FT                   /id="PRO_0000330738"
FT   TOPO_DOM        21..163
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        164..184
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        185..236
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        74
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        94
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        141
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        148
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        152
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   236 AA;  26252 MW;  DBDCFBE6624577E2 CRC64;
     MNKLITLLLA VLMIISCVYS DDVEITDDEV VQATPTSDVS FSYIFPDNQD KNFAAGSVVE
     VLVGFTNNAD KALNITHIFA SLNHPQDFSV FIQNYTRGDF GIAVEKGHHA TLAYRFVPSE
     YLEPREFGLL ISLEYQDGAQ NFTQTFFNST INITEKETSF DMDSFFLILL GLGFVGGIGY
     IVYGKMPKQK KVRTVSKVNK NAVRVETEDE TAEWLSGTSA ASSKVKSVQK VVKKNK