ID   SSRB_DICDI              Reviewed;         184 AA.
AC   Q54VI6;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   08-APR-2008, sequence version 2.
DT   25-MAY-2022, entry version 82.
DE   RecName: Full=Translocon-associated protein subunit beta;
DE            Short=TRAP-beta;
DE   AltName: Full=Signal sequence receptor subunit beta;
DE            Short=SSR-beta;
DE   Flags: Precursor;
GN   Name=ssr2; ORFNames=DDB_G0280313;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=AX4;
RX   PubMed=15010511; DOI=10.1093/nar/gkh262;
RA   Urushihara H., Morio T., Saito T., Kohara Y., Koriki E., Ochiai H.,
RA   Maeda M., Williams J.G., Takeuchi I., Tanaka Y.;
RT   "Analyses of cDNAs from growth and slug stages of Dictyostelium
RT   discoideum.";
RL   Nucleic Acids Res. 32:1647-1653(2004).
CC   -!- FUNCTION: TRAP proteins are part of a complex whose function is to bind
CC       calcium to the ER membrane and thereby regulate the retention of ER
CC       resident proteins. {ECO:0000250}.
CC   -!- SUBUNIT: Heterotrimer of TRAP-alpha, TRAP-beta and TRAP-gamma.
CC       {ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000250};
CC       Single-pass type I membrane protein {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TRAP-beta family. {ECO:0000305}.
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DR   EMBL; AAFI02000035; EAL67383.2; -; Genomic_DNA.
DR   EMBL; BJ428355; -; NOT_ANNOTATED_CDS; mRNA.
DR   RefSeq; XP_641369.2; XM_636277.2.
DR   AlphaFoldDB; Q54VI6; -.
DR   STRING; 44689.DDB0266464; -.
DR   PaxDb; Q54VI6; -.
DR   EnsemblProtists; EAL67383; EAL67383; DDB_G0280313.
DR   GeneID; 8622503; -.
DR   KEGG; ddi:DDB_G0280313; -.
DR   dictyBase; DDB_G0280313; ssr2.
DR   eggNOG; KOG3317; Eukaryota.
DR   HOGENOM; CLU_102025_1_0_1; -.
DR   InParanoid; Q54VI6; -.
DR   OMA; ILWHSSK; -.
DR   PhylomeDB; Q54VI6; -.
DR   PRO; PR:Q54VI6; -.
DR   Proteomes; UP000002195; Chromosome 3.
DR   GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0045335; C:phagocytic vesicle; HDA:dictyBase.
DR   InterPro; IPR008856; TRAP_beta.
DR   PIRSF; PIRSF016400; TRAP_beta; 1.
PE   2: Evidence at transcript level;
KW   Endoplasmic reticulum; Glycoprotein; Membrane; Reference proteome; Signal;
KW   Transmembrane; Transmembrane helix.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..184
FT                   /note="Translocon-associated protein subunit beta"
FT                   /id="PRO_0000328897"
FT   TOPO_DOM        21..147
FT                   /note="Lumenal"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        148..168
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        169..184
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        94
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   184 AA;  20673 MW;  60DB8E527C276B47 CRC64;
     MNFKTVISLF LVLFVSFVYC ENGAELLFHK KIVEGPVVGK ELPIQFIIYN VGSEPAYDIS
     FIDNDFSNAE FEFVSGSSEG KWETLAPNSQ VQTNLTVIPK KSGIYSLTST VLNYRKTQTS
     SEFTVSSAAS YSGMYVESQA DYEKRTSLLI KEWITFFVLC AGAIALPYSI STYYKKNYEN
     GIKK