SSRB_HUMAN
ID SSRB_HUMAN Reviewed; 183 AA.
AC P43308; B2R9K9; D3DVA7; Q53HI0; Q5VY95; Q5VY96; Q6IB31; Q9BWE4;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1995, sequence version 1.
DT 03-AUG-2022, entry version 173.
DE RecName: Full=Translocon-associated protein subunit beta;
DE Short=TRAP-beta;
DE AltName: Full=Signal sequence receptor subunit beta;
DE Short=SSR-beta;
DE Flags: Precursor;
GN Name=SSR2; Synonyms=TRAPB; ORFNames=HSD25;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Colon carcinoma;
RX PubMed=8286409;
RA Bodescot M., Brison O.;
RT "Cloning and sequence analysis of the beta subunit of the human translocon-
RT associated protein.";
RL Biochim. Biophys. Acta 1217:101-102(1994).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Lung;
RX PubMed=7789174; DOI=10.1159/000134036;
RA Chinen K., Sudo K., Takahashi E., Nakamura Y.;
RT "Isolation and mapping of the human beta-signal sequence receptor gene
RT (SSR2).";
RL Cytogenet. Cell Genet. 70:215-217(1995).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RA Wu N., Miao S.Y., Zhang X.D., Qiao Y., Liang G., Wang L.F.;
RT "A new spermatogenesis-related gene.";
RL Submitted (MAR-2003) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Coronary artery;
RA Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y.,
RA Tanaka A., Yokoyama S.;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [8]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [9]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [10]
RP GLYCOSYLATION AT ASN-88 AND ASN-104.
RX PubMed=12754519; DOI=10.1038/nbt827;
RA Zhang H., Li X.-J., Martin D.B., Aebersold R.;
RT "Identification and quantification of N-linked glycoproteins using
RT hydrazide chemistry, stable isotope labeling and mass spectrometry.";
RL Nat. Biotechnol. 21:660-666(2003).
RN [11]
RP INTERACTION WITH STING1.
RX PubMed=18724357; DOI=10.1038/nature07317;
RA Ishikawa H., Barber G.N.;
RT "STING is an endoplasmic reticulum adaptor that facilitates innate immune
RT signalling.";
RL Nature 455:674-678(2008).
RN [12]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-88 AND ASN-104.
RC TISSUE=Liver;
RX PubMed=19159218; DOI=10.1021/pr8008012;
RA Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.;
RT "Glycoproteomics analysis of human liver tissue by combination of multiple
RT enzyme digestion and hydrazide chemistry.";
RL J. Proteome Res. 8:651-661(2009).
RN [13]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=25944712; DOI=10.1002/pmic.201400617;
RA Vaca Jacome A.S., Rabilloud T., Schaeffer-Reiss C., Rompais M., Ayoub D.,
RA Lane L., Bairoch A., Van Dorsselaer A., Carapito C.;
RT "N-terminome analysis of the human mitochondrial proteome.";
RL Proteomics 15:2519-2524(2015).
CC -!- FUNCTION: TRAP proteins are part of a complex whose function is to bind
CC calcium to the ER membrane and thereby regulate the retention of ER
CC resident proteins.
CC -!- SUBUNIT: Heterotetramer of TRAP-alpha, TRAP-beta, TRAP-delta and TRAP-
CC gamma. Interacts with STING1. {ECO:0000269|PubMed:18724357}.
CC -!- INTERACTION:
CC P43308; Q86WV6: STING1; NbExp=4; IntAct=EBI-2822012, EBI-2800345;
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Single-pass type
CC I membrane protein.
CC -!- SIMILARITY: Belongs to the TRAP-beta family. {ECO:0000305}.
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DR EMBL; X74104; CAA52207.1; -; mRNA.
DR EMBL; D37991; BAA07206.1; -; mRNA.
DR EMBL; AY251536; AAP20059.1; -; mRNA.
DR EMBL; CR456973; CAG33254.1; -; mRNA.
DR EMBL; AK222600; BAD96320.1; -; mRNA.
DR EMBL; AK313821; BAG36556.1; -; mRNA.
DR EMBL; AL355388; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471121; EAW53010.1; -; Genomic_DNA.
DR EMBL; CH471121; EAW53011.1; -; Genomic_DNA.
DR EMBL; BC000341; AAH00341.2; -; mRNA.
DR CCDS; CCDS1126.1; -.
DR PIR; S41063; S41063.
DR RefSeq; NP_003136.1; NM_003145.3.
DR AlphaFoldDB; P43308; -.
DR BioGRID; 112624; 223.
DR DIP; DIP-60652N; -.
DR IntAct; P43308; 7.
DR MINT; P43308; -.
DR STRING; 9606.ENSP00000295702; -.
DR GlyConnect; 1840; 11 N-Linked glycans (2 sites).
DR GlyGen; P43308; 4 sites, 15 N-linked glycans (2 sites), 1 O-linked glycan (2 sites).
DR iPTMnet; P43308; -.
DR PhosphoSitePlus; P43308; -.
DR BioMuta; SSR2; -.
DR DMDM; 1174451; -.
DR jPOST; P43308; -.
DR MassIVE; P43308; -.
DR MaxQB; P43308; -.
DR PaxDb; P43308; -.
DR PeptideAtlas; P43308; -.
DR PRIDE; P43308; -.
DR ProteomicsDB; 55611; -.
DR TopDownProteomics; P43308; -.
DR Antibodypedia; 20426; 185 antibodies from 24 providers.
DR DNASU; 6746; -.
DR Ensembl; ENST00000295702.9; ENSP00000295702.4; ENSG00000163479.14.
DR Ensembl; ENST00000480567.5; ENSP00000434306.1; ENSG00000163479.14.
DR GeneID; 6746; -.
DR KEGG; hsa:6746; -.
DR MANE-Select; ENST00000295702.9; ENSP00000295702.4; NM_003145.4; NP_003136.1.
DR UCSC; uc001fmx.4; human.
DR CTD; 6746; -.
DR DisGeNET; 6746; -.
DR GeneCards; SSR2; -.
DR HGNC; HGNC:11324; SSR2.
DR HPA; ENSG00000163479; Low tissue specificity.
DR MIM; 600867; gene.
DR neXtProt; NX_P43308; -.
DR OpenTargets; ENSG00000163479; -.
DR PharmGKB; PA36148; -.
DR VEuPathDB; HostDB:ENSG00000163479; -.
DR eggNOG; KOG3317; Eukaryota.
DR GeneTree; ENSGT00390000005125; -.
DR HOGENOM; CLU_102025_1_0_1; -.
DR InParanoid; P43308; -.
DR OMA; ILWHSSK; -.
DR OrthoDB; 1286165at2759; -.
DR PhylomeDB; P43308; -.
DR TreeFam; TF314461; -.
DR PathwayCommons; P43308; -.
DR Reactome; R-HSA-1799339; SRP-dependent cotranslational protein targeting to membrane.
DR SignaLink; P43308; -.
DR BioGRID-ORCS; 6746; 53 hits in 1091 CRISPR screens.
DR ChiTaRS; SSR2; human.
DR GeneWiki; SSR2; -.
DR GenomeRNAi; 6746; -.
DR Pharos; P43308; Tbio.
DR PRO; PR:P43308; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; P43308; protein.
DR Bgee; ENSG00000163479; Expressed in body of pancreas and 212 other tissues.
DR ExpressionAtlas; P43308; baseline and differential.
DR Genevisible; P43308; HS.
DR GO; GO:0005783; C:endoplasmic reticulum; TAS:ProtInc.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016021; C:integral component of membrane; TAS:ProtInc.
DR GO; GO:0006613; P:cotranslational protein targeting to membrane; TAS:ProtInc.
DR InterPro; IPR008856; TRAP_beta.
DR PIRSF; PIRSF016400; TRAP_beta; 1.
PE 1: Evidence at protein level;
KW Endoplasmic reticulum; Glycoprotein; Membrane; Reference proteome; Signal;
KW Transmembrane; Transmembrane helix.
FT SIGNAL 1..17
FT /evidence="ECO:0000250"
FT CHAIN 18..183
FT /note="Translocon-associated protein subunit beta"
FT /id="PRO_0000033290"
FT TOPO_DOM 18..149
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 150..169
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 170..183
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 88
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:12754519,
FT ECO:0000269|PubMed:19159218"
FT CARBOHYD 104
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:12754519,
FT ECO:0000269|PubMed:19159218"
FT CONFLICT 46
FT /note="I -> T (in Ref. 6; BAD96320)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 183 AA; 20135 MW; 575715449B8586D8 CRC64;
MRLLSFVVLA LFAVTQAEEG ARLLASKSLL NRYAVEGRDL TLQYNIYNVG SSAALDVELS
DDSFPPEDFG IVSGMLNVKW DRIAPASNVS HTVVLRPLKA GYFNFTSATI TYLAQEDGPV
VIGSTSAPGQ GGILAQREFD RRFSPHFLDW AAFGVMTLPS IGIPLLLWYS SKRKYDTPKT
KKN
