ID   SSRP_MYCFP              Reviewed;         147 AA.
AC   Q9X498; C4XFU8;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   03-AUG-2022, entry version 86.
DE   RecName: Full=SsrA-binding protein {ECO:0000255|HAMAP-Rule:MF_00023};
DE   AltName: Full=Small protein B {ECO:0000255|HAMAP-Rule:MF_00023};
GN   Name=smpB {ECO:0000255|HAMAP-Rule:MF_00023}; OrderedLocusNames=MBIO_0755;
OS   Mycoplasmopsis fermentans (strain ATCC 19989 / NBRC 14854 / NCTC 10117 /
OS   PG18) (Mycoplasma fermentans).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasmopsis.
OX   NCBI_TaxID=496833;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=ATCC 19989 / NBRC 14854 / NCTC 10117 / PG18;
RX   PubMed=9916088; DOI=10.1128/iai.67.2.760-771.1999;
RA   Calcutt M.J., Kim M.F., Karpas A.B., Muehlradt P.F., Wise K.S.;
RT   "Differential posttranslational processing confers intraspecies variation
RT   of a major surface lipoprotein and a macrophage-activating lipopeptide of
RT   Mycoplasma fermentans.";
RL   Infect. Immun. 67:760-771(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 19989 / NBRC 14854 / NCTC 10117 / PG18;
RX   PubMed=19219498; DOI=10.1007/s00284-009-9362-6;
RA   Ishida N., Irikura D., Matsuda K., Sato S., Sone T., Tanaka M., Asano K.;
RT   "Molecular cloning and expression of a novel cholinephosphotransferase
RT   involved in glycoglycerophospholipid biosynthesis of Mycoplasma
RT   fermentans.";
RL   Curr. Microbiol. 58:535-540(2009).
CC   -!- FUNCTION: Required for rescue of stalled ribosomes mediated by trans-
CC       translation. Binds to transfer-messenger RNA (tmRNA), required for
CC       stable association of tmRNA with ribosomes. tmRNA and SmpB together
CC       mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is
CC       encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and
CC       it encodes a 'tag peptide', a short internal open reading frame. During
CC       trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering
CC       the A-site of stalled ribosomes, displacing the stalled mRNA. The
CC       ribosome then switches to translate the ORF on the tmRNA; the nascent
CC       peptide is terminated with the 'tag peptide' encoded by the tmRNA and
CC       targeted for degradation. The ribosome is freed to recommence
CC       translation, which seems to be the essential function of trans-
CC       translation. {ECO:0000255|HAMAP-Rule:MF_00023}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00023}.
CC       Note=The tmRNA-SmpB complex associates with stalled 70S ribosomes.
CC       {ECO:0000255|HAMAP-Rule:MF_00023}.
CC   -!- SIMILARITY: Belongs to the SmpB family. {ECO:0000255|HAMAP-
CC       Rule:MF_00023}.
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DR   EMBL; AF100324; AAD25739.1; -; Genomic_DNA.
DR   EMBL; AP009608; BAH70020.1; -; Genomic_DNA.
DR   RefSeq; WP_013527170.1; NC_021002.1.
DR   AlphaFoldDB; Q9X498; -.
DR   SMR; Q9X498; -.
DR   STRING; 496833.MBIO_0755; -.
DR   EnsemblBacteria; BAH70020; BAH70020; MBIO_0755.
DR   KEGG; mfp:MBIO_0755; -.
DR   PATRIC; fig|496833.3.peg.348; -.
DR   eggNOG; COG0691; Bacteria.
DR   HOGENOM; CLU_108953_3_1_14; -.
DR   OMA; WTNHSAR; -.
DR   OrthoDB; 1720952at2; -.
DR   Proteomes; UP000006810; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0070929; P:trans-translation; IEA:UniProtKB-UniRule.
DR   CDD; cd09294; SmpB; 1.
DR   Gene3D; 2.40.280.10; -; 1.
DR   HAMAP; MF_00023; SmpB; 1.
DR   InterPro; IPR023620; SmpB.
DR   InterPro; IPR000037; SsrA-bd_prot.
DR   InterPro; IPR020081; SsrA-bd_prot_CS.
DR   PANTHER; PTHR30308; PTHR30308; 1.
DR   Pfam; PF01668; SmpB; 1.
DR   SUPFAM; SSF74982; SSF74982; 1.
DR   TIGRFAMs; TIGR00086; smpB; 1.
DR   PROSITE; PS01317; SSRP; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; RNA-binding.
FT   CHAIN           1..147
FT                   /note="SsrA-binding protein"
FT                   /id="PRO_0000102980"
SQ   SEQUENCE   147 AA;  17475 MW;  6DFA0F5E457EFE13 CRC64;
     MKIISDNKRG MHNYKVIDKY EAGISLMGWE VKSARANTVS LLNSYCFFRK GEIFLCNAQF
     KQYMLVKCDE TRDRKLLMHK NEIVRLQSKL HKLGHATIIP SKIYFDNRSR IKIEIALVQG
     MKKTDKREEI KKRDNERYIK KVLKNVY