SSRP_SALTY
ID SSRP_SALTY Reviewed; 160 AA.
AC P0A2G1; P43658;
DT 15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 2.
DT 03-AUG-2022, entry version 90.
DE RecName: Full=SsrA-binding protein {ECO:0000255|HAMAP-Rule:MF_00023};
DE AltName: Full=Small protein B {ECO:0000255|HAMAP-Rule:MF_00023};
GN Name=smpB {ECO:0000255|HAMAP-Rule:MF_00023}; Synonyms=smqB;
GN OrderedLocusNames=STM2688;
OS Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Salmonella.
OX NCBI_TaxID=99287;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX PubMed=11677609; DOI=10.1038/35101614;
RA McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA Wilson R.K.;
RT "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL Nature 413:852-856(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 10-49, AND DISRUPTION PHENOTYPE.
RC STRAIN=ATCC 14028 / Isolate MS7481;
RX PubMed=8168923; DOI=10.1128/iai.62.5.1623-1630.1994;
RA Baumler A.J., Kusters J.G., Stojiljkovic I., Heffron F.;
RT "Salmonella typhimurium loci involved in survival within macrophages.";
RL Infect. Immun. 62:1623-1630(1994).
CC -!- FUNCTION: Required for rescue of stalled ribosomes mediated by trans-
CC translation. Binds to transfer-messenger RNA (tmRNA), required for
CC stable association of tmRNA with ribosomes. tmRNA and SmpB together
CC mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is
CC encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and
CC it encodes a 'tag peptide', a short internal open reading frame. During
CC trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering
CC the A-site of stalled ribosomes, displacing the stalled mRNA. The
CC ribosome then switches to translate the ORF on the tmRNA; the nascent
CC peptide is terminated with the 'tag peptide' encoded by the tmRNA and
CC targeted for degradation. The ribosome is freed to recommence
CC translation, which seems to be the essential function of trans-
CC translation. {ECO:0000255|HAMAP-Rule:MF_00023}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00023}.
CC Note=The tmRNA-SmpB complex associates with stalled 70S ribosomes.
CC {ECO:0000255|HAMAP-Rule:MF_00023}.
CC -!- DISRUPTION PHENOTYPE: Not essential, it has a diminished capacity to
CC survive in murine macrophages. {ECO:0000269|PubMed:8168923}.
CC -!- SIMILARITY: Belongs to the SmpB family. {ECO:0000255|HAMAP-
CC Rule:MF_00023}.
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DR EMBL; AE006468; AAL21577.1; -; Genomic_DNA.
DR EMBL; U06139; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; NP_461618.1; NC_003197.2.
DR RefSeq; WP_001518569.1; NC_003197.2.
DR AlphaFoldDB; P0A2G1; -.
DR SMR; P0A2G1; -.
DR STRING; 99287.STM2688; -.
DR PaxDb; P0A2G1; -.
DR EnsemblBacteria; AAL21577; AAL21577; STM2688.
DR GeneID; 1254211; -.
DR GeneID; 66757102; -.
DR KEGG; stm:STM2688; -.
DR PATRIC; fig|99287.12.peg.2833; -.
DR HOGENOM; CLU_108953_3_0_6; -.
DR OMA; WTNHSAR; -.
DR PhylomeDB; P0A2G1; -.
DR BioCyc; SENT99287:STM2688-MON; -.
DR PHI-base; PHI:2681; -.
DR Proteomes; UP000001014; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR GO; GO:0070929; P:trans-translation; IEA:UniProtKB-UniRule.
DR GO; GO:0070930; P:trans-translation-dependent protein tagging; IBA:GO_Central.
DR CDD; cd09294; SmpB; 1.
DR Gene3D; 2.40.280.10; -; 1.
DR HAMAP; MF_00023; SmpB; 1.
DR InterPro; IPR023620; SmpB.
DR InterPro; IPR000037; SsrA-bd_prot.
DR InterPro; IPR020081; SsrA-bd_prot_CS.
DR PANTHER; PTHR30308; PTHR30308; 1.
DR Pfam; PF01668; SmpB; 1.
DR SUPFAM; SSF74982; SSF74982; 1.
DR TIGRFAMs; TIGR00086; smpB; 1.
DR PROSITE; PS01317; SSRP; 1.
PE 3: Inferred from homology;
KW Cytoplasm; Reference proteome; RNA-binding.
FT INIT_MET 1
FT /note="Removed"
FT /evidence="ECO:0000250"
FT CHAIN 2..160
FT /note="SsrA-binding protein"
FT /id="PRO_0000103023"
FT CONFLICT 10
FT /note="G -> A (in Ref. 2; U06139)"
FT /evidence="ECO:0000305"
FT CONFLICT 20
FT /note="Missing (in Ref. 2; U06139)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 160 AA; 18232 MW; 67772270CF125CCF CRC64;
MTKKKAHKPG SATIALNKRA RHEYFIEEEF EAGLALQGWE VKSLRAGKAN IGDSYVILKD
GEAWLFGANF TPMAVASTHV VCDPTRTRKL LLNQRELDSL YGRINREGYT VVALSLYWKN
AWCKVKIGVA KGKKQHDKRS DLKEREWQLD KARIMKNAGR