位置:首页 > 蛋白库 > SSRP_SALTY
SSRP_SALTY
ID   SSRP_SALTY              Reviewed;         160 AA.
AC   P0A2G1; P43658;
DT   15-MAR-2005, integrated into UniProtKB/Swiss-Prot.
DT   23-JAN-2007, sequence version 2.
DT   03-AUG-2022, entry version 90.
DE   RecName: Full=SsrA-binding protein {ECO:0000255|HAMAP-Rule:MF_00023};
DE   AltName: Full=Small protein B {ECO:0000255|HAMAP-Rule:MF_00023};
GN   Name=smpB {ECO:0000255|HAMAP-Rule:MF_00023}; Synonyms=smqB;
GN   OrderedLocusNames=STM2688;
OS   Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Salmonella.
OX   NCBI_TaxID=99287;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=LT2 / SGSC1412 / ATCC 700720;
RX   PubMed=11677609; DOI=10.1038/35101614;
RA   McClelland M., Sanderson K.E., Spieth J., Clifton S.W., Latreille P.,
RA   Courtney L., Porwollik S., Ali J., Dante M., Du F., Hou S., Layman D.,
RA   Leonard S., Nguyen C., Scott K., Holmes A., Grewal N., Mulvaney E.,
RA   Ryan E., Sun H., Florea L., Miller W., Stoneking T., Nhan M., Waterston R.,
RA   Wilson R.K.;
RT   "Complete genome sequence of Salmonella enterica serovar Typhimurium LT2.";
RL   Nature 413:852-856(2001).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 10-49, AND DISRUPTION PHENOTYPE.
RC   STRAIN=ATCC 14028 / Isolate MS7481;
RX   PubMed=8168923; DOI=10.1128/iai.62.5.1623-1630.1994;
RA   Baumler A.J., Kusters J.G., Stojiljkovic I., Heffron F.;
RT   "Salmonella typhimurium loci involved in survival within macrophages.";
RL   Infect. Immun. 62:1623-1630(1994).
CC   -!- FUNCTION: Required for rescue of stalled ribosomes mediated by trans-
CC       translation. Binds to transfer-messenger RNA (tmRNA), required for
CC       stable association of tmRNA with ribosomes. tmRNA and SmpB together
CC       mimic tRNA shape, replacing the anticodon stem-loop with SmpB. tmRNA is
CC       encoded by the ssrA gene; the 2 termini fold to resemble tRNA(Ala) and
CC       it encodes a 'tag peptide', a short internal open reading frame. During
CC       trans-translation Ala-aminoacylated tmRNA acts like a tRNA, entering
CC       the A-site of stalled ribosomes, displacing the stalled mRNA. The
CC       ribosome then switches to translate the ORF on the tmRNA; the nascent
CC       peptide is terminated with the 'tag peptide' encoded by the tmRNA and
CC       targeted for degradation. The ribosome is freed to recommence
CC       translation, which seems to be the essential function of trans-
CC       translation. {ECO:0000255|HAMAP-Rule:MF_00023}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00023}.
CC       Note=The tmRNA-SmpB complex associates with stalled 70S ribosomes.
CC       {ECO:0000255|HAMAP-Rule:MF_00023}.
CC   -!- DISRUPTION PHENOTYPE: Not essential, it has a diminished capacity to
CC       survive in murine macrophages. {ECO:0000269|PubMed:8168923}.
CC   -!- SIMILARITY: Belongs to the SmpB family. {ECO:0000255|HAMAP-
CC       Rule:MF_00023}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; AE006468; AAL21577.1; -; Genomic_DNA.
DR   EMBL; U06139; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; NP_461618.1; NC_003197.2.
DR   RefSeq; WP_001518569.1; NC_003197.2.
DR   AlphaFoldDB; P0A2G1; -.
DR   SMR; P0A2G1; -.
DR   STRING; 99287.STM2688; -.
DR   PaxDb; P0A2G1; -.
DR   EnsemblBacteria; AAL21577; AAL21577; STM2688.
DR   GeneID; 1254211; -.
DR   GeneID; 66757102; -.
DR   KEGG; stm:STM2688; -.
DR   PATRIC; fig|99287.12.peg.2833; -.
DR   HOGENOM; CLU_108953_3_0_6; -.
DR   OMA; WTNHSAR; -.
DR   PhylomeDB; P0A2G1; -.
DR   BioCyc; SENT99287:STM2688-MON; -.
DR   PHI-base; PHI:2681; -.
DR   Proteomes; UP000001014; Chromosome.
DR   GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR   GO; GO:0003723; F:RNA binding; IBA:GO_Central.
DR   GO; GO:0070929; P:trans-translation; IEA:UniProtKB-UniRule.
DR   GO; GO:0070930; P:trans-translation-dependent protein tagging; IBA:GO_Central.
DR   CDD; cd09294; SmpB; 1.
DR   Gene3D; 2.40.280.10; -; 1.
DR   HAMAP; MF_00023; SmpB; 1.
DR   InterPro; IPR023620; SmpB.
DR   InterPro; IPR000037; SsrA-bd_prot.
DR   InterPro; IPR020081; SsrA-bd_prot_CS.
DR   PANTHER; PTHR30308; PTHR30308; 1.
DR   Pfam; PF01668; SmpB; 1.
DR   SUPFAM; SSF74982; SSF74982; 1.
DR   TIGRFAMs; TIGR00086; smpB; 1.
DR   PROSITE; PS01317; SSRP; 1.
PE   3: Inferred from homology;
KW   Cytoplasm; Reference proteome; RNA-binding.
FT   INIT_MET        1
FT                   /note="Removed"
FT                   /evidence="ECO:0000250"
FT   CHAIN           2..160
FT                   /note="SsrA-binding protein"
FT                   /id="PRO_0000103023"
FT   CONFLICT        10
FT                   /note="G -> A (in Ref. 2; U06139)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        20
FT                   /note="Missing (in Ref. 2; U06139)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   160 AA;  18232 MW;  67772270CF125CCF CRC64;
     MTKKKAHKPG SATIALNKRA RHEYFIEEEF EAGLALQGWE VKSLRAGKAN IGDSYVILKD
     GEAWLFGANF TPMAVASTHV VCDPTRTRKL LLNQRELDSL YGRINREGYT VVALSLYWKN
     AWCKVKIGVA KGKKQHDKRS DLKEREWQLD KARIMKNAGR
 
 
维奥蛋白资源库 - 中文蛋白资源 CopyRight © 2010-2024