SSUD_BACSU
ID SSUD_BACSU Reviewed; 376 AA.
AC P40402;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Alkanesulfonate monooxygenase;
DE EC=1.14.14.5;
DE AltName: Full=FMNH2-dependent aliphatic sulfonate monooxygenase;
GN Name=ssuD; Synonyms=ygcA, yzeC; OrderedLocusNames=BSU08860;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9782504; DOI=10.1099/00221287-144-9-2555;
RA van der Ploeg J.R., Cummings N.J., Leisinger T., Connerton I.F.;
RT "Bacillus subtilis genes for the utilization of sulfur from aliphatic
RT sulfonates.";
RL Microbiology 144:2555-2561(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-372.
RC STRAIN=BD99 / MS11;
RX PubMed=8011666; DOI=10.1016/0005-2728(94)90131-7;
RA Quirk P.G., Guffanti A.A., Clejan S., Cheng J., Krulwich T.A.;
RT "Isolation of Tn917 insertional mutants of Bacillus subtilis that are
RT resistant to the protonophore carbonyl cyanide m-chlorophenylhydrazone.";
RL Biochim. Biophys. Acta 1186:27-34(1994).
CC -!- FUNCTION: Catalyzes the desulfonation of aliphatic sulfonates.
CC {ECO:0000250}.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=an alkanesulfonate + FMNH2 + O2 = an aldehyde + FMN + 2 H(+) +
CC H2O + sulfite; Xref=Rhea:RHEA:23064, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:17359,
CC ChEBI:CHEBI:17478, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC ChEBI:CHEBI:134249; EC=1.14.14.5;
CC -!- INDUCTION: Repressed by sulfate or cysteine.
CC -!- SIMILARITY: Belongs to the SsuD family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=CAB07523.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; Z93102; CAB07523.1; ALT_INIT; Genomic_DNA.
DR EMBL; AL009126; CAB12714.2; -; Genomic_DNA.
DR EMBL; L16808; AAA64350.1; -; Genomic_DNA.
DR PIR; D69817; D69817.
DR RefSeq; NP_388766.2; NC_000964.3.
DR RefSeq; WP_003245787.1; NZ_JNCM01000035.1.
DR AlphaFoldDB; P40402; -.
DR SMR; P40402; -.
DR STRING; 224308.BSU08860; -.
DR PaxDb; P40402; -.
DR PRIDE; P40402; -.
DR EnsemblBacteria; CAB12714; CAB12714; BSU_08860.
DR GeneID; 939733; -.
DR KEGG; bsu:BSU08860; -.
DR PATRIC; fig|224308.179.peg.956; -.
DR eggNOG; COG2141; Bacteria.
DR InParanoid; P40402; -.
DR OMA; NIFWFLP; -.
DR PhylomeDB; P40402; -.
DR BioCyc; BSUB:BSU08860-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0008726; F:alkanesulfonate monooxygenase activity; IBA:GO_Central.
DR GO; GO:0046306; P:alkanesulfonate catabolic process; IBA:GO_Central.
DR Gene3D; 3.20.20.30; -; 1.
DR HAMAP; MF_01229; Alkanesulf_monooxygen; 1.
DR InterPro; IPR019911; Alkanesulphonate_mOase_FMN-dep.
DR InterPro; IPR011251; Luciferase-like_dom.
DR InterPro; IPR036661; Luciferase-like_sf.
DR Pfam; PF00296; Bac_luciferase; 1.
DR SUPFAM; SSF51679; SSF51679; 1.
DR TIGRFAMs; TIGR03565; alk_sulf_monoox; 1.
PE 2: Evidence at transcript level;
KW Flavoprotein; FMN; Monooxygenase; Oxidoreductase; Reference proteome.
FT CHAIN 1..376
FT /note="Alkanesulfonate monooxygenase"
FT /id="PRO_0000216703"
SQ SEQUENCE 376 AA; 41802 MW; 0F1FA3DC77BCF4F5 CRC64;
MEILWFIPTH GDARYLGSES DGRTADHLYF KQVAQAADRL GYTGVLLPTG RSCEDPWLTA
SALAGETKDL KFLVAVRPGL MQPSLAARMT STLDRISDGR LLINVVAGGD PYELAGDGLF
ISHDERYEAT DEFLTVWRRL LQGETVSYEG KHIKVENSNL LFPPQQEPHP PIYFGGSSQA
GIEAAAKHTD VYLTWGEPPE QVKEKIERVK KQAAKEGRSV RFGIRLHVIA RETEQEAWEA
AERLISHLDD DTIAKAQAAL SRYDSSGQQR MAVLHQGDRT KLEISPNLWA GIGLVRGGAG
TALVGDPQTI ADRIAEYQAL GIESFIFSGY PHLEEAYYFA ELVFPLLPFE NDRTRKLQNK
RGEAVGNTYF VKEKNA