ID   SSUD_BUTSP              Reviewed;         382 AA.
AC   Q9KHR3;
DT   10-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 68.
DE   RecName: Full=Alkanesulfonate monooxygenase {ECO:0000255|HAMAP-Rule:MF_01229};
DE            EC=1.14.14.5 {ECO:0000255|HAMAP-Rule:MF_01229};
DE   AltName: Full=FMNH2-dependent aliphatic sulfonate monooxygenase {ECO:0000255|HAMAP-Rule:MF_01229};
GN   Name=ssuD {ECO:0000255|HAMAP-Rule:MF_01229};
OS   Buttiauxella sp. (strain PNBS).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Buttiauxella; unclassified Buttiauxella.
OX   NCBI_TaxID=129886;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RA   Kertesz M.A., Sialm M.;
RT   "Sulfur-controlled aryl desulfonation phenotypes in Pseudomonas spp. and
RT   other soil isolates, and conservation of the sulfonate monooxygenase gene
RT   (ssuD).";
RL   Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the desulfonation of aliphatic sulfonates.
CC       {ECO:0000255|HAMAP-Rule:MF_01229}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an alkanesulfonate + FMNH2 + O2 = an aldehyde + FMN + 2 H(+) +
CC         H2O + sulfite; Xref=Rhea:RHEA:23064, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:17359,
CC         ChEBI:CHEBI:17478, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC         ChEBI:CHEBI:134249; EC=1.14.14.5; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01229};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000255|HAMAP-Rule:MF_01229}.
CC   -!- MISCELLANEOUS: FMNH(2) which is absolutely required for this enzymatic
CC       reaction, is provided by SsuE. {ECO:0000255|HAMAP-Rule:MF_01229}.
CC   -!- SIMILARITY: Belongs to the SsuD family. {ECO:0000255|HAMAP-
CC       Rule:MF_01229}.
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DR   EMBL; AF250869; AAF81710.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9KHR3; -.
DR   SMR; Q9KHR3; -.
DR   GO; GO:0008726; F:alkanesulfonate monooxygenase activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.20.20.30; -; 1.
DR   HAMAP; MF_01229; Alkanesulf_monooxygen; 1.
DR   InterPro; IPR019911; Alkanesulphonate_mOase_FMN-dep.
DR   InterPro; IPR011251; Luciferase-like_dom.
DR   InterPro; IPR036661; Luciferase-like_sf.
DR   Pfam; PF00296; Bac_luciferase; 1.
DR   SUPFAM; SSF51679; SSF51679; 1.
DR   TIGRFAMs; TIGR03565; alk_sulf_monoox; 1.
PE   3: Inferred from homology;
KW   Flavoprotein; FMN; Monooxygenase; Oxidoreductase.
FT   CHAIN           1..382
FT                   /note="Alkanesulfonate monooxygenase"
FT                   /id="PRO_0000216705"
SQ   SEQUENCE   382 AA;  41587 MW;  0E363AA42C95226B CRC64;
     MSLNIFWFLP THGDGKYLGT SDGARAVDHG YLQQIAQAAD RLGFGGVLIP TGRSCEDSWL
     VAASLIPVTQ HLKFLVALRP GIISPTVAAR QAATLDRLSN GRALFNLVTG GDPDELAGDG
     LHLNHQERYE ASVEFTRIWR KVLEGENVDY DGKHIQVKGA KLLYPPIQQP RPPLYFGGSS
     EAAQDLAAEQ VELYLTWGEP PAAVAEKIAQ VREKAAAQGR EVRFGIRLHV IVRETNEEAW
     AAADRLISHL DDETIARAQA SLARFDSVGQ QRMAALHGGN RDNLEVSPNL WAGVGLVRGG
     AGTALVGDGP TVAARVKEYA DLGIDTFIFS GYPHLEESYR VAELLFPHLD VQRPEQPKTR
     GYVSPFGEMV ANDILPKSVS QS