ID   SSUD_CUPNH              Reviewed;         387 AA.
AC   Q0K9I0;
DT   05-FEB-2008, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2006, sequence version 1.
DT   25-MAY-2022, entry version 96.
DE   RecName: Full=Alkanesulfonate monooxygenase {ECO:0000255|HAMAP-Rule:MF_01229};
DE            EC=1.14.14.5 {ECO:0000255|HAMAP-Rule:MF_01229};
DE   AltName: Full=FMNH2-dependent aliphatic sulfonate monooxygenase {ECO:0000255|HAMAP-Rule:MF_01229};
GN   Name=ssuD {ECO:0000255|HAMAP-Rule:MF_01229}; OrderedLocusNames=H16_A2244;
OS   Cupriavidus necator (strain ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442
OS   / H16 / Stanier 337) (Ralstonia eutropha).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Cupriavidus.
OX   NCBI_TaxID=381666;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 17699 / DSM 428 / KCTC 22496 / NCIMB 10442 / H16 / Stanier 337;
RX   PubMed=16964242; DOI=10.1038/nbt1244;
RA   Pohlmann A., Fricke W.F., Reinecke F., Kusian B., Liesegang H., Cramm R.,
RA   Eitinger T., Ewering C., Poetter M., Schwartz E., Strittmatter A., Voss I.,
RA   Gottschalk G., Steinbuechel A., Friedrich B., Bowien B.;
RT   "Genome sequence of the bioplastic-producing 'Knallgas' bacterium Ralstonia
RT   eutropha H16.";
RL   Nat. Biotechnol. 24:1257-1262(2006).
CC   -!- FUNCTION: Catalyzes the desulfonation of aliphatic sulfonates.
CC       {ECO:0000255|HAMAP-Rule:MF_01229}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an alkanesulfonate + FMNH2 + O2 = an aldehyde + FMN + 2 H(+) +
CC         H2O + sulfite; Xref=Rhea:RHEA:23064, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:17359,
CC         ChEBI:CHEBI:17478, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC         ChEBI:CHEBI:134249; EC=1.14.14.5; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01229};
CC   -!- SIMILARITY: Belongs to the SsuD family. {ECO:0000255|HAMAP-
CC       Rule:MF_01229}.
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DR   EMBL; AM260479; CAJ93341.1; -; Genomic_DNA.
DR   RefSeq; WP_011615572.1; NZ_CP039287.1.
DR   AlphaFoldDB; Q0K9I0; -.
DR   SMR; Q0K9I0; -.
DR   STRING; 381666.H16_A2244; -.
DR   EnsemblBacteria; CAJ93341; CAJ93341; H16_A2244.
DR   GeneID; 57644373; -.
DR   KEGG; reh:H16_A2244; -.
DR   eggNOG; COG2141; Bacteria.
DR   HOGENOM; CLU_027853_1_0_4; -.
DR   OMA; NIFWFLP; -.
DR   OrthoDB; 919913at2; -.
DR   Proteomes; UP000008210; Chromosome 1.
DR   GO; GO:0008726; F:alkanesulfonate monooxygenase activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.20.20.30; -; 1.
DR   HAMAP; MF_01229; Alkanesulf_monooxygen; 1.
DR   InterPro; IPR019911; Alkanesulphonate_mOase_FMN-dep.
DR   InterPro; IPR011251; Luciferase-like_dom.
DR   InterPro; IPR036661; Luciferase-like_sf.
DR   Pfam; PF00296; Bac_luciferase; 1.
DR   SUPFAM; SSF51679; SSF51679; 1.
DR   TIGRFAMs; TIGR03565; alk_sulf_monoox; 1.
PE   3: Inferred from homology;
KW   Flavoprotein; FMN; Monooxygenase; Oxidoreductase; Reference proteome.
FT   CHAIN           1..387
FT                   /note="Alkanesulfonate monooxygenase"
FT                   /id="PRO_1000066832"
SQ   SEQUENCE   387 AA;  41893 MW;  18305833343DE89B CRC64;
     MQVFWFIPTH GDSRYLGTSE GAREVSFDYL KQVAVAADTL GYEGVLIPTG RSCEDPWVAA
     SALAAVTQRL KFLVAVRPGL MAPTLAARMA ATFDRISNGR LLINLVTGGD TAELEGDGLF
     LDHTARYEAS AEFLRIWRQV LAASHDGDKV DYEGKHLSVK GATVLYPPLQ QPHPPVYFGG
     SSAPAHALAG EQVDTYLTWG EPPADVAQKL DDVRRQAARH GRTVKFGIRL HVIVRETEAA
     AWAAADDLIS RLDDETVARA QAVFAKMDSE GQRRMAALHA GGARRTREAL EISPNLWAGV
     GLVRGGAGTA LVGDPHTVAE RMREYAELGI DTFVLSGYPH LEEAYRFAEL VFPLLPRSVR
     DKLPGKVLSG PFGEVMATGI VPRAAQS