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SSUD_PSEPU
ID   SSUD_PSEPU              Reviewed;         382 AA.
AC   O85764; Q8KZQ8; Q9KHR0;
DT   15-JUL-1999, integrated into UniProtKB/Swiss-Prot.
DT   10-JAN-2003, sequence version 2.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Alkanesulfonate monooxygenase;
DE            EC=1.14.14.5;
DE   AltName: Full=FMNH2-dependent aliphatic sulfonate monooxygenase;
GN   Name=ssuD; Synonyms=dsuD;
OS   Pseudomonas putida (Arthrobacter siderocapsulatus).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=303;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DSM 6884 / S-313;
RX   PubMed=10781557; DOI=10.1128/jb.182.10.2869-2878.2000;
RA   Kahnert A., Vermeij P., Wietek C., James P., Leisinger T., Kertesz M.A.;
RT   "The ssu locus plays a key role in organosulfur metabolism in Pseudomonas
RT   putida S-313.";
RL   J. Bacteriol. 182:2869-2878(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Rot18;
RA   Kertesz M.A., Sialm M.;
RT   "Sulfur-controlled aryl desulfonation phenotypes in Pseudomonas spp. and
RT   other soil isolates, and conservation of the sulfonate monooxygenase gene
RT   (ssuD).";
RL   Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DS1;
RX   PubMed=12835925; DOI=10.1007/s00253-003-1233-7;
RA   Endoh T., Kasuga K., Horinouchi M., Yoshida T., Habe H., Nojiri H.,
RA   Omori T.;
RT   "Characterization and identification of genes essential for dimethyl
RT   sulfide utilization in Pseudomonas putida strain DS1.";
RL   Appl. Microbiol. Biotechnol. 62:83-91(2003).
CC   -!- FUNCTION: Catalyzes the desulfonation of aliphatic sulfonates. Seems
CC       also to be involved in the desulfurization of aromatic sulfonates.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an alkanesulfonate + FMNH2 + O2 = an aldehyde + FMN + 2 H(+) +
CC         H2O + sulfite; Xref=Rhea:RHEA:23064, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:17359,
CC         ChEBI:CHEBI:17478, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC         ChEBI:CHEBI:134249; EC=1.14.14.5;
CC   -!- INDUCTION: Repressed by sulfate, cysteine, or thiocyanate.
CC   -!- MISCELLANEOUS: FMNH(2) which is absolutely required for this enzymatic
CC       reaction, is provided by SsuE.
CC   -!- SIMILARITY: Belongs to the SsuD family. {ECO:0000305}.
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DR   EMBL; AF075709; AAC31905.1; -; Genomic_DNA.
DR   EMBL; AF250870; AAF81713.1; -; Genomic_DNA.
DR   EMBL; AB086390; BAC00973.1; -; Genomic_DNA.
DR   AlphaFoldDB; O85764; -.
DR   SMR; O85764; -.
DR   STRING; 1240350.AMZE01000010_gene3811; -.
DR   eggNOG; COG2141; Bacteria.
DR   BioCyc; MetaCyc:MON-14252; -.
DR   BRENDA; 1.14.14.34; 5092.
DR   GO; GO:0008726; F:alkanesulfonate monooxygenase activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.20.20.30; -; 1.
DR   HAMAP; MF_01229; Alkanesulf_monooxygen; 1.
DR   InterPro; IPR019911; Alkanesulphonate_mOase_FMN-dep.
DR   InterPro; IPR011251; Luciferase-like_dom.
DR   InterPro; IPR036661; Luciferase-like_sf.
DR   Pfam; PF00296; Bac_luciferase; 1.
DR   SUPFAM; SSF51679; SSF51679; 1.
DR   TIGRFAMs; TIGR03565; alk_sulf_monoox; 1.
PE   2: Evidence at transcript level;
KW   Flavoprotein; FMN; Monooxygenase; Oxidoreductase.
FT   CHAIN           1..382
FT                   /note="Alkanesulfonate monooxygenase"
FT                   /id="PRO_0000216713"
FT   CONFLICT        21
FT                   /note="S -> N (in Ref. 2; AAF81713)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        22
FT                   /note="E -> D (in Ref. 1; AAC31905)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        70
FT                   /note="Q -> E (in Ref. 1; AAC31905)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        95
FT                   /note="L -> F (in Ref. 2; AAF81713)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        147
FT                   /note="V -> N (in Ref. 1; AAC31905)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        167
FT                   /note="I -> V (in Ref. 1; AAC31905)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        181
FT                   /note="E -> D (in Ref. 2; AAF81713)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        202
FT                   /note="S -> A (in Ref. 1 and 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        221
FT                   /note="E -> Q (in Ref. 2; AAF81713)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        244
FT                   /note="D -> E (in Ref. 2; AAF81713)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        245
FT                   /note="K -> R (in Ref. 1; AAC31905)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        278
FT                   /note="N -> G (in Ref. 1; AAC31905)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        280
FT                   /note="N -> K (in Ref. 2; AAF81713)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        283
FT                   /note="K -> N (in Ref. 1; AAC31905)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        283
FT                   /note="K -> Q (in Ref. 2; AAF81713)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        321
FT                   /note="E -> D (in Ref. 1; AAC31905)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        349
FT                   /note="L -> I (in Ref. 1; AAC31905)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        356
FT                   /note="Q -> I (in Ref. 2; AAF81713)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        357
FT                   /note="A -> P (in Ref. 1; AAC31905)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        359
FT                   /note="T -> S (in Ref. 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        360
FT                   /note="S -> G (in Ref. 1 and 2)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        370..371
FT                   /note="VA -> C (in Ref. 2; AAF81713)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        380
FT                   /note="A -> S (in Ref. 1; AAC31905)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   382 AA;  41553 MW;  B9117539DFF5DDAD CRC64;
     MSLNIFWFLP THGDGKYLGT SEGARAVDHG YLQQIAQAAD RLGFGGVLIP TGRSCEDSWL
     VAASLIPVTQ RLKFLVALRP GIISPTVAAR QAATLDRLSN GRALFNLVTG GDPDELAGDG
     LHLNHQERYE ASVEFTRIWR KVLEGEVVDY DGKHIQVKGA KLLYPPIQQP RPPLYFGGSS
     EAAQDLAAEQ VELYLTWGEP PSAVAEKIAQ VREKAAAQGR EVRFGIRLHV IVRETNEEAW
     AAADKLISHL DDDTIARAQA SLARFDSVGQ QRMAALHNGN RDKLEVSPNL WAGVGLVRGG
     AGTALVGDGP TVAARVKEYA ELGIDTFIFS GYPHLEESYR VAELLFPHLD VQRPEQAKTS
     GYVSPFGEMV ANDILPKSVA QS
 
 
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