ID   SSUD_PSESP              Reviewed;         382 AA.
AC   Q9KHS2; Q9KHR6; Q9KHR9;
DT   10-JAN-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2000, sequence version 1.
DT   25-MAY-2022, entry version 72.
DE   RecName: Full=Alkanesulfonate monooxygenase {ECO:0000255|HAMAP-Rule:MF_01229};
DE            EC=1.14.14.5 {ECO:0000255|HAMAP-Rule:MF_01229};
DE   AltName: Full=FMNH2-dependent aliphatic sulfonate monooxygenase {ECO:0000255|HAMAP-Rule:MF_01229};
GN   Name=ssuD {ECO:0000255|HAMAP-Rule:MF_01229};
OS   Pseudomonas sp.
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=306;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=Blue1, PSBZ, and Tar2A;
RA   Kertesz M.A., Sialm M.;
RT   "Sulfur-controlled aryl desulfonation phenotypes in Pseudomonas spp. and
RT   other soil isolates, and conservation of the sulfonate monooxygenase gene
RT   (ssuD).";
RL   Submitted (MAR-2000) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the desulfonation of aliphatic sulfonates.
CC       {ECO:0000255|HAMAP-Rule:MF_01229}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an alkanesulfonate + FMNH2 + O2 = an aldehyde + FMN + 2 H(+) +
CC         H2O + sulfite; Xref=Rhea:RHEA:23064, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:17359,
CC         ChEBI:CHEBI:17478, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC         ChEBI:CHEBI:134249; EC=1.14.14.5; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01229};
CC   -!- SIMILARITY: Belongs to the SsuD family. {ECO:0000255|HAMAP-
CC       Rule:MF_01229}.
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DR   EMBL; AF250866; AAF81701.1; -; Genomic_DNA.
DR   EMBL; AF250867; AAF81704.1; -; Genomic_DNA.
DR   EMBL; AF250868; AAF81707.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q9KHS2; -.
DR   SMR; Q9KHS2; -.
DR   GO; GO:0008726; F:alkanesulfonate monooxygenase activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.20.20.30; -; 1.
DR   HAMAP; MF_01229; Alkanesulf_monooxygen; 1.
DR   InterPro; IPR019911; Alkanesulphonate_mOase_FMN-dep.
DR   InterPro; IPR011251; Luciferase-like_dom.
DR   InterPro; IPR036661; Luciferase-like_sf.
DR   Pfam; PF00296; Bac_luciferase; 1.
DR   SUPFAM; SSF51679; SSF51679; 1.
DR   TIGRFAMs; TIGR03565; alk_sulf_monoox; 1.
PE   3: Inferred from homology;
KW   Flavoprotein; FMN; Monooxygenase; Oxidoreductase.
FT   CHAIN           1..382
FT                   /note="Alkanesulfonate monooxygenase"
FT                   /id="PRO_0000216715"
FT   VARIANT         31
FT                   /note="Y -> H (in strain: PSBZ)"
FT   VARIANT         70..71
FT                   /note="ER -> QH (in strain: Blue1)"
FT   VARIANT         105..106
FT                   /note="FN -> IP (in strain: PSBZ)"
FT   VARIANT         167
FT                   /note="V -> I (in strain: Blue1)"
FT   VARIANT         253
FT                   /note="D -> E (in strain: Blue1)"
FT   VARIANT         347
FT                   /note="L -> P (in strain: PSBZ and Blue1)"
FT   VARIANT         349
FT                   /note="I -> L (in strain: Blue1)"
SQ   SEQUENCE   382 AA;  41496 MW;  59BEAF5B0059D770 CRC64;
     MSLNIFWFLP THGDGKYLGT SDGARAVDHG YLQQIAQAAD RLGFGGVLIP TGRSCEDSWL
     VAASLIPVTE RLKFLVALRP GIISPTVAAR QAATLDRLSN GRALFNLVTG GDPDELAGDG
     LHLNHQERYE ASVEFTRIWR KVLEGENVDY DGKHIQVKGA KLLYPPVQQP RPPLYFGGSS
     EAAQDLAAEQ VELYLTWGEP PAAVAEKIAQ VREKAAAQGR EVRFGIRLHV IVRETNEEAW
     AAADRLISHL DDDTIARAQA SLARFDSVGQ QRMAALHGGN RDNLEVSPNL WAGVGLVRGG
     AGTALVGDGP TVAARVKEYA DLGIDTFIFS GYPHLEESYR VAELLFLHID VQRPEQPKTG
     GYVSPFGEMV ANDILPKSVS QS