ID   SSUD_RALPJ              Reviewed;         387 AA.
AC   B2UAU3;
DT   14-APR-2009, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-2008, sequence version 1.
DT   25-MAY-2022, entry version 85.
DE   RecName: Full=Alkanesulfonate monooxygenase {ECO:0000255|HAMAP-Rule:MF_01229};
DE            EC=1.14.14.5 {ECO:0000255|HAMAP-Rule:MF_01229};
DE   AltName: Full=FMNH2-dependent aliphatic sulfonate monooxygenase {ECO:0000255|HAMAP-Rule:MF_01229};
GN   Name=ssuD {ECO:0000255|HAMAP-Rule:MF_01229}; OrderedLocusNames=Rpic_1215;
OS   Ralstonia pickettii (strain 12J).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Burkholderiaceae; Ralstonia.
OX   NCBI_TaxID=402626;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=12J;
RA   Lucas S., Copeland A., Lapidus A., Glavina del Rio T., Dalin E., Tice H.,
RA   Bruce D., Goodwin L., Pitluck S., Meincke L., Brettin T., Detter J.C.,
RA   Han C., Kuske C.R., Schmutz J., Larimer F., Land M., Hauser L.,
RA   Kyrpides N., Mikhailova N., Marsh T., Richardson P.;
RT   "Complete sequence of chromosome 1 of Ralstonia pickettii 12J.";
RL   Submitted (MAY-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes the desulfonation of aliphatic sulfonates.
CC       {ECO:0000255|HAMAP-Rule:MF_01229}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=an alkanesulfonate + FMNH2 + O2 = an aldehyde + FMN + 2 H(+) +
CC         H2O + sulfite; Xref=Rhea:RHEA:23064, ChEBI:CHEBI:15377,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:17359,
CC         ChEBI:CHEBI:17478, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210,
CC         ChEBI:CHEBI:134249; EC=1.14.14.5; Evidence={ECO:0000255|HAMAP-
CC         Rule:MF_01229};
CC   -!- SIMILARITY: Belongs to the SsuD family. {ECO:0000255|HAMAP-
CC       Rule:MF_01229}.
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DR   EMBL; CP001068; ACD26359.1; -; Genomic_DNA.
DR   RefSeq; WP_012435416.1; NC_010682.1.
DR   AlphaFoldDB; B2UAU3; -.
DR   SMR; B2UAU3; -.
DR   STRING; 402626.Rpic_1215; -.
DR   EnsemblBacteria; ACD26359; ACD26359; Rpic_1215.
DR   KEGG; rpi:Rpic_1215; -.
DR   PATRIC; fig|402626.5.peg.2417; -.
DR   eggNOG; COG2141; Bacteria.
DR   HOGENOM; CLU_027853_1_0_4; -.
DR   OMA; NIFWFLP; -.
DR   OrthoDB; 919913at2; -.
DR   GO; GO:0008726; F:alkanesulfonate monooxygenase activity; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.20.20.30; -; 1.
DR   HAMAP; MF_01229; Alkanesulf_monooxygen; 1.
DR   InterPro; IPR019911; Alkanesulphonate_mOase_FMN-dep.
DR   InterPro; IPR011251; Luciferase-like_dom.
DR   InterPro; IPR036661; Luciferase-like_sf.
DR   Pfam; PF00296; Bac_luciferase; 1.
DR   SUPFAM; SSF51679; SSF51679; 1.
DR   TIGRFAMs; TIGR03565; alk_sulf_monoox; 1.
PE   3: Inferred from homology;
KW   Flavoprotein; FMN; Monooxygenase; Oxidoreductase.
FT   CHAIN           1..387
FT                   /note="Alkanesulfonate monooxygenase"
FT                   /id="PRO_1000139627"
SQ   SEQUENCE   387 AA;  41920 MW;  E6AD107E80919BE4 CRC64;
     MQVFWFIPTH GDSRYLGTAE GARQVDHTYL QQVAVAADTL GYEGVLIPTG RSCEDPWVVA
     ASLIPVTKRL RFLVAVRPGL MAPTLAARMA ATYDRLSNGR LLVNLVTGGD PAELAGDGLF
     LDHAQRYEAS EEFIRIWRET LAASHEGAAL DYTGKHLSVK GAKVLYPPVQ RPHPPVYFGG
     SSEAAHELAA EQVDSYLTWG EPPAAVAEKI ADVRARAAKH GRTVRFGIRL HVIVRETEDE
     AWAAADKLIS KLDDDTVARA QEAFRKMDSA GQQRMAALHA NGVKRTRADL EISPNLWAGV
     GLVRGGAGTA LVGDPQTVAA RMKEYADLGI DTFVLSGYPH LEEAYRFAEL VFPLLPRSVR
     DKLPGNVLNG PFGEVIATGI VPRVAAS